Biogenesis of the inner membrane complex is dependent on vesicular transport by the alveolate specific GTPase Rab11B.

Apicomplexan parasites belong to a recently recognised group of protozoa referred to as Alveolata. These protists contain membranous sacs (alveoli) beneath the plasma membrane, termed the Inner Membrane Complex (IMC) in the case of Apicomplexa. During parasite replication the IMC is formed de novo w...

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Autores principales:	Carolina Agop-Nersesian, Saskia Egarter, Gordon Langsley, Bernardo J Foth, David J P Ferguson, Markus Meissner
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/7d5e007436f9431e84cc736d34cfd817
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spelling	oai:doaj.org-article:7d5e007436f9431e84cc736d34cfd8172021-12-02T19:59:44ZBiogenesis of the inner membrane complex is dependent on vesicular transport by the alveolate specific GTPase Rab11B.1553-73661553-737410.1371/journal.ppat.1001029https://doaj.org/article/7d5e007436f9431e84cc736d34cfd8172010-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20686666/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Apicomplexan parasites belong to a recently recognised group of protozoa referred to as Alveolata. These protists contain membranous sacs (alveoli) beneath the plasma membrane, termed the Inner Membrane Complex (IMC) in the case of Apicomplexa. During parasite replication the IMC is formed de novo within the mother cell in a process described as internal budding. We hypothesized that an alveolate specific factor is involved in the specific transport of vesicles from the Golgi to the IMC and identified the small GTPase Rab11B as an alveolate specific Rab-GTPase that localises to the growing end of the IMC during replication of Toxoplasma gondii. Conditional interference with Rab11B function leads to a profound defect in IMC biogenesis, indicating that Rab11B is required for the transport of Golgi derived vesicles to the nascent IMC of the daughter cell. Curiously, a block in IMC biogenesis did not affect formation of sub-pellicular microtubules, indicating that IMC biogenesis and formation of sub-pellicular microtubules is not mechanistically linked. We propose a model where Rab11B specifically transports vesicles derived from the Golgi to the immature IMC of the growing daughter parasites.Carolina Agop-NersesianSaskia EgarterGordon LangsleyBernardo J FothDavid J P FergusonMarkus MeissnerPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 6, Iss 7, p e1001029 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Carolina Agop-Nersesian Saskia Egarter Gordon Langsley Bernardo J Foth David J P Ferguson Markus Meissner Biogenesis of the inner membrane complex is dependent on vesicular transport by the alveolate specific GTPase Rab11B.
description	Apicomplexan parasites belong to a recently recognised group of protozoa referred to as Alveolata. These protists contain membranous sacs (alveoli) beneath the plasma membrane, termed the Inner Membrane Complex (IMC) in the case of Apicomplexa. During parasite replication the IMC is formed de novo within the mother cell in a process described as internal budding. We hypothesized that an alveolate specific factor is involved in the specific transport of vesicles from the Golgi to the IMC and identified the small GTPase Rab11B as an alveolate specific Rab-GTPase that localises to the growing end of the IMC during replication of Toxoplasma gondii. Conditional interference with Rab11B function leads to a profound defect in IMC biogenesis, indicating that Rab11B is required for the transport of Golgi derived vesicles to the nascent IMC of the daughter cell. Curiously, a block in IMC biogenesis did not affect formation of sub-pellicular microtubules, indicating that IMC biogenesis and formation of sub-pellicular microtubules is not mechanistically linked. We propose a model where Rab11B specifically transports vesicles derived from the Golgi to the immature IMC of the growing daughter parasites.
format	article
author	Carolina Agop-Nersesian Saskia Egarter Gordon Langsley Bernardo J Foth David J P Ferguson Markus Meissner
author_facet	Carolina Agop-Nersesian Saskia Egarter Gordon Langsley Bernardo J Foth David J P Ferguson Markus Meissner
author_sort	Carolina Agop-Nersesian
title	Biogenesis of the inner membrane complex is dependent on vesicular transport by the alveolate specific GTPase Rab11B.
title_short	Biogenesis of the inner membrane complex is dependent on vesicular transport by the alveolate specific GTPase Rab11B.
title_full	Biogenesis of the inner membrane complex is dependent on vesicular transport by the alveolate specific GTPase Rab11B.
title_fullStr	Biogenesis of the inner membrane complex is dependent on vesicular transport by the alveolate specific GTPase Rab11B.
title_full_unstemmed	Biogenesis of the inner membrane complex is dependent on vesicular transport by the alveolate specific GTPase Rab11B.
title_sort	biogenesis of the inner membrane complex is dependent on vesicular transport by the alveolate specific gtpase rab11b.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/7d5e007436f9431e84cc736d34cfd817
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Biogenesis of the inner membrane complex is dependent on vesicular transport by the alveolate specific GTPase Rab11B.

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