Functional studies of the yeast med5, med15 and med16 mediator tail subunits.

The yeast Mediator complex can be divided into three modules, designated Head, Middle and Tail. Tail comprises the Med2, Med3, Med5, Med15 and Med16 protein subunits, which are all encoded by genes that are individually non-essential for viability. In cells lacking Med16, Tail is displaced from Head...

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Autores principales:	Miriam Larsson, Hanna Uvell, Jenny Sandström, Patrik Rydén, Luke A Selth, Stefan Björklund
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7d5e8673105e489293c1bf4d5d04e382
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spelling	oai:doaj.org-article:7d5e8673105e489293c1bf4d5d04e3822021-11-18T08:58:24ZFunctional studies of the yeast med5, med15 and med16 mediator tail subunits.1932-620310.1371/journal.pone.0073137https://doaj.org/article/7d5e8673105e489293c1bf4d5d04e3822013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23991176/?tool=EBIhttps://doaj.org/toc/1932-6203The yeast Mediator complex can be divided into three modules, designated Head, Middle and Tail. Tail comprises the Med2, Med3, Med5, Med15 and Med16 protein subunits, which are all encoded by genes that are individually non-essential for viability. In cells lacking Med16, Tail is displaced from Head and Middle. However, inactivation of MED5/MED15 and MED15/MED16 are synthetically lethal, indicating that Tail performs essential functions as a separate complex even when it is not bound to Middle and Head. We have used the N-Degron method to create temperature-sensitive (ts) mutants in the Mediator tail subunits Med5, Med15 and Med16 to study the immediate effects on global gene expression when each subunit is individually inactivated, and when Med5/15 or Med15/16 are inactivated together. We identify 25 genes in each double mutant that show a significant change in expression when compared to the corresponding single mutants and to the wild type strain. Importantly, 13 of the 25 identified genes are common for both double mutants. We also find that all strains in which MED15 is inactivated show down-regulation of genes that have been identified as targets for the Ace2 transcriptional activator protein, which is important for progression through the G1 phase of the cell cycle. Supporting this observation, we demonstrate that loss of Med15 leads to a G1 arrest phenotype. Collectively, these findings provide insight into the function of the Mediator Tail module.Miriam LarssonHanna UvellJenny SandströmPatrik RydénLuke A SelthStefan BjörklundPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e73137 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Miriam Larsson Hanna Uvell Jenny Sandström Patrik Rydén Luke A Selth Stefan Björklund Functional studies of the yeast med5, med15 and med16 mediator tail subunits.
description	The yeast Mediator complex can be divided into three modules, designated Head, Middle and Tail. Tail comprises the Med2, Med3, Med5, Med15 and Med16 protein subunits, which are all encoded by genes that are individually non-essential for viability. In cells lacking Med16, Tail is displaced from Head and Middle. However, inactivation of MED5/MED15 and MED15/MED16 are synthetically lethal, indicating that Tail performs essential functions as a separate complex even when it is not bound to Middle and Head. We have used the N-Degron method to create temperature-sensitive (ts) mutants in the Mediator tail subunits Med5, Med15 and Med16 to study the immediate effects on global gene expression when each subunit is individually inactivated, and when Med5/15 or Med15/16 are inactivated together. We identify 25 genes in each double mutant that show a significant change in expression when compared to the corresponding single mutants and to the wild type strain. Importantly, 13 of the 25 identified genes are common for both double mutants. We also find that all strains in which MED15 is inactivated show down-regulation of genes that have been identified as targets for the Ace2 transcriptional activator protein, which is important for progression through the G1 phase of the cell cycle. Supporting this observation, we demonstrate that loss of Med15 leads to a G1 arrest phenotype. Collectively, these findings provide insight into the function of the Mediator Tail module.
format	article
author	Miriam Larsson Hanna Uvell Jenny Sandström Patrik Rydén Luke A Selth Stefan Björklund
author_facet	Miriam Larsson Hanna Uvell Jenny Sandström Patrik Rydén Luke A Selth Stefan Björklund
author_sort	Miriam Larsson
title	Functional studies of the yeast med5, med15 and med16 mediator tail subunits.
title_short	Functional studies of the yeast med5, med15 and med16 mediator tail subunits.
title_full	Functional studies of the yeast med5, med15 and med16 mediator tail subunits.
title_fullStr	Functional studies of the yeast med5, med15 and med16 mediator tail subunits.
title_full_unstemmed	Functional studies of the yeast med5, med15 and med16 mediator tail subunits.
title_sort	functional studies of the yeast med5, med15 and med16 mediator tail subunits.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/7d5e8673105e489293c1bf4d5d04e382
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Functional studies of the yeast med5, med15 and med16 mediator tail subunits.

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