The carbon storage regulator (Csr) system exerts a nutrient-specific control over central metabolism in Escherichia coli strain Nissle 1917.
The role of the post-transcriptional carbon storage regulator (Csr) system in nutrient utilization and in the control of the central metabolism in E. coli reference commensal strain Nissle 1917 was investigated. Analysis of the growth capabilities of mutants altered for various components of the Csr...
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Public Library of Science (PLoS)
2013
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oai:doaj.org-article:7d5fdfa8c0424b4c8e2a00e5b23aac9b2021-11-18T07:40:48ZThe carbon storage regulator (Csr) system exerts a nutrient-specific control over central metabolism in Escherichia coli strain Nissle 1917.1932-620310.1371/journal.pone.0066386https://doaj.org/article/7d5fdfa8c0424b4c8e2a00e5b23aac9b2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23840455/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The role of the post-transcriptional carbon storage regulator (Csr) system in nutrient utilization and in the control of the central metabolism in E. coli reference commensal strain Nissle 1917 was investigated. Analysis of the growth capabilities of mutants altered for various components of the Csr system (csrA51, csrB, csrC and csrD mutations) showed that only the protein CsrA - the key component of the system - exerts a marked role in carbon nutrition. Attenuation of CsrA activity in the csrA51 mutant affects the growth efficiency on a broad range of physiologically relevant carbon sources, including compounds utilized by the Entner-Doudoroff (ED) pathway. Detailed investigations of the metabolomes and fluxomes of mutants and wild-type cells grown on carbon sources representative of glycolysis and of the ED pathway (glucose and gluconate, respectively), revealed significant re-adjusting of central carbon metabolism for both compounds in the csrA51 mutant. However, the metabolic re-adjusting observed on gluconate was strikingly different from that observed on glucose, indicating a nutrient-specific control of metabolism by the Csr system.Olga RevellesPierre MillardJean-Philippe NougayrèdeUlrich DobrindtEric OswaldFabien LétisseJean-Charles PortaisPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e66386 (2013) |
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Medicine R Science Q Olga Revelles Pierre Millard Jean-Philippe Nougayrède Ulrich Dobrindt Eric Oswald Fabien Létisse Jean-Charles Portais The carbon storage regulator (Csr) system exerts a nutrient-specific control over central metabolism in Escherichia coli strain Nissle 1917. |
| description |
The role of the post-transcriptional carbon storage regulator (Csr) system in nutrient utilization and in the control of the central metabolism in E. coli reference commensal strain Nissle 1917 was investigated. Analysis of the growth capabilities of mutants altered for various components of the Csr system (csrA51, csrB, csrC and csrD mutations) showed that only the protein CsrA - the key component of the system - exerts a marked role in carbon nutrition. Attenuation of CsrA activity in the csrA51 mutant affects the growth efficiency on a broad range of physiologically relevant carbon sources, including compounds utilized by the Entner-Doudoroff (ED) pathway. Detailed investigations of the metabolomes and fluxomes of mutants and wild-type cells grown on carbon sources representative of glycolysis and of the ED pathway (glucose and gluconate, respectively), revealed significant re-adjusting of central carbon metabolism for both compounds in the csrA51 mutant. However, the metabolic re-adjusting observed on gluconate was strikingly different from that observed on glucose, indicating a nutrient-specific control of metabolism by the Csr system. |
| format |
article |
| author |
Olga Revelles Pierre Millard Jean-Philippe Nougayrède Ulrich Dobrindt Eric Oswald Fabien Létisse Jean-Charles Portais |
| author_facet |
Olga Revelles Pierre Millard Jean-Philippe Nougayrède Ulrich Dobrindt Eric Oswald Fabien Létisse Jean-Charles Portais |
| author_sort |
Olga Revelles |
| title |
The carbon storage regulator (Csr) system exerts a nutrient-specific control over central metabolism in Escherichia coli strain Nissle 1917. |
| title_short |
The carbon storage regulator (Csr) system exerts a nutrient-specific control over central metabolism in Escherichia coli strain Nissle 1917. |
| title_full |
The carbon storage regulator (Csr) system exerts a nutrient-specific control over central metabolism in Escherichia coli strain Nissle 1917. |
| title_fullStr |
The carbon storage regulator (Csr) system exerts a nutrient-specific control over central metabolism in Escherichia coli strain Nissle 1917. |
| title_full_unstemmed |
The carbon storage regulator (Csr) system exerts a nutrient-specific control over central metabolism in Escherichia coli strain Nissle 1917. |
| title_sort |
carbon storage regulator (csr) system exerts a nutrient-specific control over central metabolism in escherichia coli strain nissle 1917. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/7d5fdfa8c0424b4c8e2a00e5b23aac9b |
| work_keys_str_mv |
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| _version_ |
1718423106719055872 |