Molecular diversity of benthic ctenophores (Coeloplanidae)

Abstract Coeloplanidae, the largest family of benthic ctenophores, comprises 33 species, all described based on traditional morphological characteristics, such as coloration, length, and number of aboral papillae, which are highly variable and can be affected by fixation methods and environmental co...

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Autores principales:	Ada Alamaru, Bert W. Hoeksema, Sancia E. T. van der Meij, Dorothée Huchon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7d62ad1117b346698124c5878668badb
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spelling	oai:doaj.org-article:7d62ad1117b346698124c5878668badb2021-12-02T15:05:28ZMolecular diversity of benthic ctenophores (Coeloplanidae)10.1038/s41598-017-06505-42045-2322https://doaj.org/article/7d62ad1117b346698124c5878668badb2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06505-4https://doaj.org/toc/2045-2322Abstract Coeloplanidae, the largest family of benthic ctenophores, comprises 33 species, all described based on traditional morphological characteristics, such as coloration, length, and number of aboral papillae, which are highly variable and can be affected by fixation methods and environmental conditions. Thus, there is a need for reliable genetic markers to complement the morphological identifications at the species level. Here, we analyzed 95 specimens from 11 morphologically distinct species of benthic ctenophores from the Red Sea and Sulu Sea, and tested selected regions of four genetic markers (ITS1, 18S rRNA, 28S rRNA and COI) for their ability to differentiate between species. We show that the barcoding region of the mitochondrial gene, cytochrome oxidase subunit I (COI), is highly variable among species of Coeloplanidae, and effectively discriminates between species in this family. The average Kimura-2-parameter (K2P) distance between species-level clades was 10%, while intraspecific variation was ~30 times lower (0.36%). COI-based phylogeny supported the delineation of four recently described new species from the Red Sea. The other nuclear markers tested were found to be too conserved in order to separate between species. We conclude that COI is a potential molecular barcode for the family Coeloplanidae and suggest to test it in pelagic ctenophores.Ada AlamaruBert W. HoeksemaSancia E. T. van der MeijDorothée HuchonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ada Alamaru Bert W. Hoeksema Sancia E. T. van der Meij Dorothée Huchon Molecular diversity of benthic ctenophores (Coeloplanidae)
description	Abstract Coeloplanidae, the largest family of benthic ctenophores, comprises 33 species, all described based on traditional morphological characteristics, such as coloration, length, and number of aboral papillae, which are highly variable and can be affected by fixation methods and environmental conditions. Thus, there is a need for reliable genetic markers to complement the morphological identifications at the species level. Here, we analyzed 95 specimens from 11 morphologically distinct species of benthic ctenophores from the Red Sea and Sulu Sea, and tested selected regions of four genetic markers (ITS1, 18S rRNA, 28S rRNA and COI) for their ability to differentiate between species. We show that the barcoding region of the mitochondrial gene, cytochrome oxidase subunit I (COI), is highly variable among species of Coeloplanidae, and effectively discriminates between species in this family. The average Kimura-2-parameter (K2P) distance between species-level clades was 10%, while intraspecific variation was ~30 times lower (0.36%). COI-based phylogeny supported the delineation of four recently described new species from the Red Sea. The other nuclear markers tested were found to be too conserved in order to separate between species. We conclude that COI is a potential molecular barcode for the family Coeloplanidae and suggest to test it in pelagic ctenophores.
format	article
author	Ada Alamaru Bert W. Hoeksema Sancia E. T. van der Meij Dorothée Huchon
author_facet	Ada Alamaru Bert W. Hoeksema Sancia E. T. van der Meij Dorothée Huchon
author_sort	Ada Alamaru
title	Molecular diversity of benthic ctenophores (Coeloplanidae)
title_short	Molecular diversity of benthic ctenophores (Coeloplanidae)
title_full	Molecular diversity of benthic ctenophores (Coeloplanidae)
title_fullStr	Molecular diversity of benthic ctenophores (Coeloplanidae)
title_full_unstemmed	Molecular diversity of benthic ctenophores (Coeloplanidae)
title_sort	molecular diversity of benthic ctenophores (coeloplanidae)
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7d62ad1117b346698124c5878668badb
work_keys_str_mv	AT adaalamaru moleculardiversityofbenthicctenophorescoeloplanidae AT bertwhoeksema moleculardiversityofbenthicctenophorescoeloplanidae AT sanciaetvandermeij moleculardiversityofbenthicctenophorescoeloplanidae AT dorotheehuchon moleculardiversityofbenthicctenophorescoeloplanidae
_version_	1718388841272836096

Molecular diversity of benthic ctenophores (Coeloplanidae)

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