Gastrodin modified polyurethane conduit promotes nerve repair via optimizing Schwann cells function

Peripheral nerve regeneration and functional recovery remain a major clinical challenge. Nerve guidance conduit (NGC) that can regulate biological behavior of Schwann cells (SCs) and facilitate axonal regeneration through microenvironmental remodeling is beneficial for nerve regeneration and functio...

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Autores principales:	Hongcai Yang, Qing Li, Limei Li, Shaochun Chen, Yu Zhao, Yingrui Hu, Lu wang, Xiaoqian Lan, Lianmei Zhong, Di Lu
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2022
Materias:	Gastrodin/PU SCs biological behavior Anti-inflammatory Peripheral nerve regeneration Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/7d6aabc9846c45828711c4ca10ac9619
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spelling	oai:doaj.org-article:7d6aabc9846c45828711c4ca10ac96192021-11-04T04:35:38ZGastrodin modified polyurethane conduit promotes nerve repair via optimizing Schwann cells function2452-199X10.1016/j.bioactmat.2021.06.020https://doaj.org/article/7d6aabc9846c45828711c4ca10ac96192022-02-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2452199X21003066https://doaj.org/toc/2452-199XPeripheral nerve regeneration and functional recovery remain a major clinical challenge. Nerve guidance conduit (NGC) that can regulate biological behavior of Schwann cells (SCs) and facilitate axonal regeneration through microenvironmental remodeling is beneficial for nerve regeneration and functional recovery. Gastrodin, a main constituent of a Chinese traditional herbal medicine, has been known to display several biological and pharmacological properties, especially antioxidative, anti‐inflammatory and nerve regeneration. Herein, polyurethane (PU) NGCs modified by different weight ratio of Gastrodin (0, 1 and 5 wt%) were designed for sequential and sustainable drug release, that created a favorable microenvironment for nerve regeneration. The scaffold showed suitable pore structure and biocompatibility in vitro, and evidently promoted morphological and functional recovery of regenerated sciatic nerves in vivo. Compared to the PU and 1%Gastrodin/PU scaffolds, the 5%Gastrodin/PU significantly enhanced the proliferation, migration and myelination of SCs and up-regulated expression of neurotrophic factors, as well as induction of the differentiation of PC12 cells. Interestingly, the obvious anti-inflammatory response was observed in 5%Gastrodin/PU by reduced expression of TNF-α and iNOS, which also evidenced by the few fibrous capsule formation in the subcutaneous implantation. Such a construct presented a similarity to autograft in vivo repairing a 10 mm sciatic nerve defects. It was able to not only boost the regenerated area of nerve and microvascular network, but also facilitate functional axons growth and remyelination, leading to highly improved functional restoration. These findings demonstrate that the 5%Gastrodin/PU NGC efficiently promotes nerve regeneration, indicating their potential for use in peripheral nerve regeneration applications.Hongcai YangQing LiLimei LiShaochun ChenYu ZhaoYingrui HuLu wangXiaoqian LanLianmei ZhongDi LuKeAi Communications Co., Ltd.articleGastrodin/PUSCs biological behaviorAnti-inflammatoryPeripheral nerve regenerationMaterials of engineering and construction. Mechanics of materialsTA401-492Biology (General)QH301-705.5ENBioactive Materials, Vol 8, Iss , Pp 355-367 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Gastrodin/PU SCs biological behavior Anti-inflammatory Peripheral nerve regeneration Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
spellingShingle	Gastrodin/PU SCs biological behavior Anti-inflammatory Peripheral nerve regeneration Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5 Hongcai Yang Qing Li Limei Li Shaochun Chen Yu Zhao Yingrui Hu Lu wang Xiaoqian Lan Lianmei Zhong Di Lu Gastrodin modified polyurethane conduit promotes nerve repair via optimizing Schwann cells function
description	Peripheral nerve regeneration and functional recovery remain a major clinical challenge. Nerve guidance conduit (NGC) that can regulate biological behavior of Schwann cells (SCs) and facilitate axonal regeneration through microenvironmental remodeling is beneficial for nerve regeneration and functional recovery. Gastrodin, a main constituent of a Chinese traditional herbal medicine, has been known to display several biological and pharmacological properties, especially antioxidative, anti‐inflammatory and nerve regeneration. Herein, polyurethane (PU) NGCs modified by different weight ratio of Gastrodin (0, 1 and 5 wt%) were designed for sequential and sustainable drug release, that created a favorable microenvironment for nerve regeneration. The scaffold showed suitable pore structure and biocompatibility in vitro, and evidently promoted morphological and functional recovery of regenerated sciatic nerves in vivo. Compared to the PU and 1%Gastrodin/PU scaffolds, the 5%Gastrodin/PU significantly enhanced the proliferation, migration and myelination of SCs and up-regulated expression of neurotrophic factors, as well as induction of the differentiation of PC12 cells. Interestingly, the obvious anti-inflammatory response was observed in 5%Gastrodin/PU by reduced expression of TNF-α and iNOS, which also evidenced by the few fibrous capsule formation in the subcutaneous implantation. Such a construct presented a similarity to autograft in vivo repairing a 10 mm sciatic nerve defects. It was able to not only boost the regenerated area of nerve and microvascular network, but also facilitate functional axons growth and remyelination, leading to highly improved functional restoration. These findings demonstrate that the 5%Gastrodin/PU NGC efficiently promotes nerve regeneration, indicating their potential for use in peripheral nerve regeneration applications.
format	article
author	Hongcai Yang Qing Li Limei Li Shaochun Chen Yu Zhao Yingrui Hu Lu wang Xiaoqian Lan Lianmei Zhong Di Lu
author_facet	Hongcai Yang Qing Li Limei Li Shaochun Chen Yu Zhao Yingrui Hu Lu wang Xiaoqian Lan Lianmei Zhong Di Lu
author_sort	Hongcai Yang
title	Gastrodin modified polyurethane conduit promotes nerve repair via optimizing Schwann cells function
title_short	Gastrodin modified polyurethane conduit promotes nerve repair via optimizing Schwann cells function
title_full	Gastrodin modified polyurethane conduit promotes nerve repair via optimizing Schwann cells function
title_fullStr	Gastrodin modified polyurethane conduit promotes nerve repair via optimizing Schwann cells function
title_full_unstemmed	Gastrodin modified polyurethane conduit promotes nerve repair via optimizing Schwann cells function
title_sort	gastrodin modified polyurethane conduit promotes nerve repair via optimizing schwann cells function
publisher	KeAi Communications Co., Ltd.
publishDate	2022
url	https://doaj.org/article/7d6aabc9846c45828711c4ca10ac9619
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Gastrodin modified polyurethane conduit promotes nerve repair via optimizing Schwann cells function

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