The Stability of Dentin Surface Biobarrier Consisting of Mesoporous Delivery System on Dentinal Tubule Occlusion and Streptococcus Mutans Biofilm Inhibition
Jian Yu,* Luyao Yi,* Rui Guo, Jingmei Guo, Hongye Yang, Cui Huang The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, People’s R...
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Dove Medical Press
2021
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oai:doaj.org-article:7d6b4c716b5f42f79acf1e2d524aba432021-12-02T15:41:25ZThe Stability of Dentin Surface Biobarrier Consisting of Mesoporous Delivery System on Dentinal Tubule Occlusion and Streptococcus Mutans Biofilm Inhibition1178-2013https://doaj.org/article/7d6b4c716b5f42f79acf1e2d524aba432021-04-01T00:00:00Zhttps://www.dovepress.com/the-stability-of-dentin-surface-biobarrier-consisting-of-mesoporous-de-peer-reviewed-fulltext-article-IJNhttps://doaj.org/toc/1178-2013Jian Yu,* Luyao Yi,* Rui Guo, Jingmei Guo, Hongye Yang, Cui Huang The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hongye Yang; Cui HuangThe State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, #237 Luoyu Road, Wuhan, 430079, Hubei Province, People’s Republic of ChinaEmail yanghongye@whu.edu.cn; huangcui@whu.edu.cnBackground: The dentin exposure always leads to dentin hypersensitivity and/or caries. Given the dentin’s tubular structure and low mineralization degree, reestablishing an effective biobarrier to stably protect dentin remains significantly challenging. This study reports a versatile dentin surface biobarrier consisting of a mesoporous silica-based epigallocatechin-3-gallate (EGCG)/nanohydroxyapatite delivery system and evaluates its stability on the dentinal tubule occlusion and the Streptococcus mutans (S. mutans) biofilm inhibition.Materials and Methods: The mesoporous delivery system was fabricated and characterized. Sensitive dentin discs were prepared and randomly allocated to three groups: 1, control group; 2, casein phosphopeptide–amorphous calcium phosphate (CPP–ACP) group; and 3, the mesoporous delivery system group. The dentin permeability, dentinal tubule occlusion, acid and abrasion resistance, and S. mutans biofilm inhibition were determined for 1 week and 1 month. The in vitro release profiles of EGCG, Ca, and P were also monitored.Results: The mesoporous delivery system held the ability to sustainably release EGCG, Ca, and P and could persistently occlude dentinal tubules with acid and abrasion resistance, reduce the dentin permeability, and inhibit the S. mutans biofilm formation for up to 1 month compared with the two other groups. The system provided prolonged stability to combat oral adverse challenges and served as an effective surface biobarrier to protect the exposed dentin.Conclusion: The establishment of the dentin surface biobarrier consisting of a mesoporous delivery system indicates a promising strategy for the prevention and the management of dentin hypersensitivity and caries after enamel loss.Keywords: epigallocatechin-3-gallate, nanohydroxyapatite, mesoporous silica, dentin, biofilmYu JYi LGuo RGuo JYang HHuang CDove Medical Pressarticleepigallocatechin-3-gallatenanohydroxyapatitemesoporous silicadentinbiofilmMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 3041-3057 (2021) |
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DOAJ |
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DOAJ |
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EN |
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epigallocatechin-3-gallate nanohydroxyapatite mesoporous silica dentin biofilm Medicine (General) R5-920 |
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epigallocatechin-3-gallate nanohydroxyapatite mesoporous silica dentin biofilm Medicine (General) R5-920 Yu J Yi L Guo R Guo J Yang H Huang C The Stability of Dentin Surface Biobarrier Consisting of Mesoporous Delivery System on Dentinal Tubule Occlusion and Streptococcus Mutans Biofilm Inhibition |
| description |
Jian Yu,* Luyao Yi,* Rui Guo, Jingmei Guo, Hongye Yang, Cui Huang The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hongye Yang; Cui HuangThe State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, #237 Luoyu Road, Wuhan, 430079, Hubei Province, People’s Republic of ChinaEmail yanghongye@whu.edu.cn; huangcui@whu.edu.cnBackground: The dentin exposure always leads to dentin hypersensitivity and/or caries. Given the dentin’s tubular structure and low mineralization degree, reestablishing an effective biobarrier to stably protect dentin remains significantly challenging. This study reports a versatile dentin surface biobarrier consisting of a mesoporous silica-based epigallocatechin-3-gallate (EGCG)/nanohydroxyapatite delivery system and evaluates its stability on the dentinal tubule occlusion and the Streptococcus mutans (S. mutans) biofilm inhibition.Materials and Methods: The mesoporous delivery system was fabricated and characterized. Sensitive dentin discs were prepared and randomly allocated to three groups: 1, control group; 2, casein phosphopeptide–amorphous calcium phosphate (CPP–ACP) group; and 3, the mesoporous delivery system group. The dentin permeability, dentinal tubule occlusion, acid and abrasion resistance, and S. mutans biofilm inhibition were determined for 1 week and 1 month. The in vitro release profiles of EGCG, Ca, and P were also monitored.Results: The mesoporous delivery system held the ability to sustainably release EGCG, Ca, and P and could persistently occlude dentinal tubules with acid and abrasion resistance, reduce the dentin permeability, and inhibit the S. mutans biofilm formation for up to 1 month compared with the two other groups. The system provided prolonged stability to combat oral adverse challenges and served as an effective surface biobarrier to protect the exposed dentin.Conclusion: The establishment of the dentin surface biobarrier consisting of a mesoporous delivery system indicates a promising strategy for the prevention and the management of dentin hypersensitivity and caries after enamel loss.Keywords: epigallocatechin-3-gallate, nanohydroxyapatite, mesoporous silica, dentin, biofilm |
| format |
article |
| author |
Yu J Yi L Guo R Guo J Yang H Huang C |
| author_facet |
Yu J Yi L Guo R Guo J Yang H Huang C |
| author_sort |
Yu J |
| title |
The Stability of Dentin Surface Biobarrier Consisting of Mesoporous Delivery System on Dentinal Tubule Occlusion and Streptococcus Mutans Biofilm Inhibition |
| title_short |
The Stability of Dentin Surface Biobarrier Consisting of Mesoporous Delivery System on Dentinal Tubule Occlusion and Streptococcus Mutans Biofilm Inhibition |
| title_full |
The Stability of Dentin Surface Biobarrier Consisting of Mesoporous Delivery System on Dentinal Tubule Occlusion and Streptococcus Mutans Biofilm Inhibition |
| title_fullStr |
The Stability of Dentin Surface Biobarrier Consisting of Mesoporous Delivery System on Dentinal Tubule Occlusion and Streptococcus Mutans Biofilm Inhibition |
| title_full_unstemmed |
The Stability of Dentin Surface Biobarrier Consisting of Mesoporous Delivery System on Dentinal Tubule Occlusion and Streptococcus Mutans Biofilm Inhibition |
| title_sort |
stability of dentin surface biobarrier consisting of mesoporous delivery system on dentinal tubule occlusion and streptococcus mutans biofilm inhibition |
| publisher |
Dove Medical Press |
| publishDate |
2021 |
| url |
https://doaj.org/article/7d6b4c716b5f42f79acf1e2d524aba43 |
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