3D reconstruction of structures of hatched larva and young juvenile of the larvacean Oikopleura dioica using SBF-SEM

Abstract The larvacean Oikopleura dioica is a planktonic chordate and an emerging model organism with a short life cycle of 5 days that belongs toTunicata (Urochordata), the sister clade of vertebrates. It is characterized by the rapid development of a tadpole-shaped body. Organ formation in the tru...

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Autores principales:	Hiroki Nishida, Nobuhiko Ohno, Federico Caicci, Lucia Manni
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7d6cde5217634513919bfa4e69bfe821
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spelling	oai:doaj.org-article:7d6cde5217634513919bfa4e69bfe8212021-12-02T13:34:32Z3D reconstruction of structures of hatched larva and young juvenile of the larvacean Oikopleura dioica using SBF-SEM10.1038/s41598-021-83706-y2045-2322https://doaj.org/article/7d6cde5217634513919bfa4e69bfe8212021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83706-yhttps://doaj.org/toc/2045-2322Abstract The larvacean Oikopleura dioica is a planktonic chordate and an emerging model organism with a short life cycle of 5 days that belongs toTunicata (Urochordata), the sister clade of vertebrates. It is characterized by the rapid development of a tadpole-shaped body. Organ formation in the trunk proceeds within 7 h after the hatching of the tailbud larvae at 3 h after fertilization (hpf) and is completed at 10 hpf, giving rise to fully functional juveniles as miniature adult form. Serial block face scanning electron microscopy was used to acquire ~ 2000 serial transverse section images of a 3 hpf larva and a 10 hpf juvenile to characterize the structures and cellular composition of the trunk and organs using 3D images and movies. Germ cells were found to fuse and establish a central syncytial cell in the gonad as early as 10 hpf. Larval development gave rise to functional organs after several rounds of cell division through trunk morphogenesis. The feature would make O. dioica ideal for analyzing cellular behaviors during morphogenetic processes using live imaging. The detailed descriptions of the larvae and juveniles provided in this study can be utilized as the start and end points of organ morphogenesis in this rapidly developing organism.Hiroki NishidaNobuhiko OhnoFederico CaicciLucia ManniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hiroki Nishida Nobuhiko Ohno Federico Caicci Lucia Manni 3D reconstruction of structures of hatched larva and young juvenile of the larvacean Oikopleura dioica using SBF-SEM
description	Abstract The larvacean Oikopleura dioica is a planktonic chordate and an emerging model organism with a short life cycle of 5 days that belongs toTunicata (Urochordata), the sister clade of vertebrates. It is characterized by the rapid development of a tadpole-shaped body. Organ formation in the trunk proceeds within 7 h after the hatching of the tailbud larvae at 3 h after fertilization (hpf) and is completed at 10 hpf, giving rise to fully functional juveniles as miniature adult form. Serial block face scanning electron microscopy was used to acquire ~ 2000 serial transverse section images of a 3 hpf larva and a 10 hpf juvenile to characterize the structures and cellular composition of the trunk and organs using 3D images and movies. Germ cells were found to fuse and establish a central syncytial cell in the gonad as early as 10 hpf. Larval development gave rise to functional organs after several rounds of cell division through trunk morphogenesis. The feature would make O. dioica ideal for analyzing cellular behaviors during morphogenetic processes using live imaging. The detailed descriptions of the larvae and juveniles provided in this study can be utilized as the start and end points of organ morphogenesis in this rapidly developing organism.
format	article
author	Hiroki Nishida Nobuhiko Ohno Federico Caicci Lucia Manni
author_facet	Hiroki Nishida Nobuhiko Ohno Federico Caicci Lucia Manni
author_sort	Hiroki Nishida
title	3D reconstruction of structures of hatched larva and young juvenile of the larvacean Oikopleura dioica using SBF-SEM
title_short	3D reconstruction of structures of hatched larva and young juvenile of the larvacean Oikopleura dioica using SBF-SEM
title_full	3D reconstruction of structures of hatched larva and young juvenile of the larvacean Oikopleura dioica using SBF-SEM
title_fullStr	3D reconstruction of structures of hatched larva and young juvenile of the larvacean Oikopleura dioica using SBF-SEM
title_full_unstemmed	3D reconstruction of structures of hatched larva and young juvenile of the larvacean Oikopleura dioica using SBF-SEM
title_sort	3d reconstruction of structures of hatched larva and young juvenile of the larvacean oikopleura dioica using sbf-sem
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7d6cde5217634513919bfa4e69bfe821
work_keys_str_mv	AT hirokinishida 3dreconstructionofstructuresofhatchedlarvaandyoungjuvenileofthelarvaceanoikopleuradioicausingsbfsem AT nobuhikoohno 3dreconstructionofstructuresofhatchedlarvaandyoungjuvenileofthelarvaceanoikopleuradioicausingsbfsem AT federicocaicci 3dreconstructionofstructuresofhatchedlarvaandyoungjuvenileofthelarvaceanoikopleuradioicausingsbfsem AT luciamanni 3dreconstructionofstructuresofhatchedlarvaandyoungjuvenileofthelarvaceanoikopleuradioicausingsbfsem
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3D reconstruction of structures of hatched larva and young juvenile of the larvacean Oikopleura dioica using SBF-SEM

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