Structural and functional microbial diversity along a eutrophication gradient of interconnected lakes undergoing anthropopressure

Abstract We present the results of an analysis of the 16S rRNA-based taxonomical structure of bacteria together with an analysis of carbon source utilization ability using EcoPlate (Biolog, USA) metabolic fingerprinting assessment against the backdrop of physicochemical parameters in fifteen interco...

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Autores principales:	Bartosz Kiersztyn, Ryszard Chróst, Tomasz Kaliński, Waldemar Siuda, Aleksandra Bukowska, Grzegorz Kowalczyk, Karolina Grabowska
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7d6d0fb76eab4473a933ffeee39769db
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spelling	oai:doaj.org-article:7d6d0fb76eab4473a933ffeee39769db2021-12-02T15:08:21ZStructural and functional microbial diversity along a eutrophication gradient of interconnected lakes undergoing anthropopressure10.1038/s41598-019-47577-82045-2322https://doaj.org/article/7d6d0fb76eab4473a933ffeee39769db2019-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-47577-8https://doaj.org/toc/2045-2322Abstract We present the results of an analysis of the 16S rRNA-based taxonomical structure of bacteria together with an analysis of carbon source utilization ability using EcoPlate (Biolog, USA) metabolic fingerprinting assessment against the backdrop of physicochemical parameters in fifteen interconnected lakes. The lakes exhibit a wide spectrum of trophic gradients and undergo different intensities of anthropopressure. Sequences of V3–V4 16S rRNA genes binned by taxonomic assignment to family indicated that bacterial communities in the highly eutrophicated lakes were distinctly different from the bacterial communities in the meso-eutrophic lakes (ANOSIM r = 0.99, p = 0.0002) and were characterized by higher richness and more diverse taxonomical structure. Representatives of the Actinobacteria, Proteobacteria, Cyanobacteria, Planctomycetes, Verrucomicrobia, Bacteroides phyla predominated. In most cases their relative abundance was significantly correlated with lake trophic state. We found no similar clear relationship of community-level physiological profiling with lake trophic state. However, we found some significant links between the taxonomic and metabolic structure of the microbes in the studied lakes (Mantel’s correlation r = 0.22, p = 0.006). The carbon source utilization ability of the studied microorganisms was affected not only by the taxonomic groups present in the lakes but also by various characteristics like a high PO4 3− concentration inhibiting the utilization of phosphorylated carbon.Bartosz KiersztynRyszard ChróstTomasz KalińskiWaldemar SiudaAleksandra BukowskaGrzegorz KowalczykKarolina GrabowskaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-14 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bartosz Kiersztyn Ryszard Chróst Tomasz Kaliński Waldemar Siuda Aleksandra Bukowska Grzegorz Kowalczyk Karolina Grabowska Structural and functional microbial diversity along a eutrophication gradient of interconnected lakes undergoing anthropopressure
description	Abstract We present the results of an analysis of the 16S rRNA-based taxonomical structure of bacteria together with an analysis of carbon source utilization ability using EcoPlate (Biolog, USA) metabolic fingerprinting assessment against the backdrop of physicochemical parameters in fifteen interconnected lakes. The lakes exhibit a wide spectrum of trophic gradients and undergo different intensities of anthropopressure. Sequences of V3–V4 16S rRNA genes binned by taxonomic assignment to family indicated that bacterial communities in the highly eutrophicated lakes were distinctly different from the bacterial communities in the meso-eutrophic lakes (ANOSIM r = 0.99, p = 0.0002) and were characterized by higher richness and more diverse taxonomical structure. Representatives of the Actinobacteria, Proteobacteria, Cyanobacteria, Planctomycetes, Verrucomicrobia, Bacteroides phyla predominated. In most cases their relative abundance was significantly correlated with lake trophic state. We found no similar clear relationship of community-level physiological profiling with lake trophic state. However, we found some significant links between the taxonomic and metabolic structure of the microbes in the studied lakes (Mantel’s correlation r = 0.22, p = 0.006). The carbon source utilization ability of the studied microorganisms was affected not only by the taxonomic groups present in the lakes but also by various characteristics like a high PO4 3− concentration inhibiting the utilization of phosphorylated carbon.
format	article
author	Bartosz Kiersztyn Ryszard Chróst Tomasz Kaliński Waldemar Siuda Aleksandra Bukowska Grzegorz Kowalczyk Karolina Grabowska
author_facet	Bartosz Kiersztyn Ryszard Chróst Tomasz Kaliński Waldemar Siuda Aleksandra Bukowska Grzegorz Kowalczyk Karolina Grabowska
author_sort	Bartosz Kiersztyn
title	Structural and functional microbial diversity along a eutrophication gradient of interconnected lakes undergoing anthropopressure
title_short	Structural and functional microbial diversity along a eutrophication gradient of interconnected lakes undergoing anthropopressure
title_full	Structural and functional microbial diversity along a eutrophication gradient of interconnected lakes undergoing anthropopressure
title_fullStr	Structural and functional microbial diversity along a eutrophication gradient of interconnected lakes undergoing anthropopressure
title_full_unstemmed	Structural and functional microbial diversity along a eutrophication gradient of interconnected lakes undergoing anthropopressure
title_sort	structural and functional microbial diversity along a eutrophication gradient of interconnected lakes undergoing anthropopressure
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/7d6d0fb76eab4473a933ffeee39769db
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Structural and functional microbial diversity along a eutrophication gradient of interconnected lakes undergoing anthropopressure

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