Dissect the immunity using cytokine profiling and NF-kB target gene analysis in systemic inflammatory minipig model.

Minipigs have remarkably similar physiology to humans, therefore, they it can be a good animal model for inflammation study. Thus, the conventional (serum chemistry, histopathology) and novel analytic tools [immune cell identification in tissue, cytokine level in peripheral blood mononuclear cells (...

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Autores principales:	Han Na Suh, Young Kyu Kim, Ju Young Lee, Goo-Hwa Kang, Jeong Ho Hwang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7d6e686d8924434995ec7347605bf324
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spelling	oai:doaj.org-article:7d6e686d8924434995ec7347605bf3242021-12-02T20:15:50ZDissect the immunity using cytokine profiling and NF-kB target gene analysis in systemic inflammatory minipig model.1932-620310.1371/journal.pone.0252947https://doaj.org/article/7d6e686d8924434995ec7347605bf3242021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252947https://doaj.org/toc/1932-6203Minipigs have remarkably similar physiology to humans, therefore, they it can be a good animal model for inflammation study. Thus, the conventional (serum chemistry, histopathology) and novel analytic tools [immune cell identification in tissue, cytokine level in peripheral blood mononuclear cells (PBMC) and serum, NF-kB target gene analysis in tissue] were applied to determine inflammation in Chicago Miniature Swine (CMS) minipig. Lipopolysaccharide (LPS)-induced acute systemic inflammation caused liver and kidney damage in serum chemistry and histopathology. Immunohistochemistry (IHC) also showed an increase of immune cell distribution in spleen and lung during inflammation. Moreover, NF-kB-target gene expression was upregulated in lung and kidney in acute inflammation and in heart, liver, and intestine in chronic inflammation. Cytokine mRNA was elevated in PBMC under acute inflammation along with elevated absolute cytokine levels in serum. Overall, LPS-mediated systemic inflammation affects the various organs, and can be detected by IHC of immune cells, gene analysis in PBMC, and measuring the absolute cytokine in serum along with conventional inflammation analytic tools.Han Na SuhYoung Kyu KimJu Young LeeGoo-Hwa KangJeong Ho HwangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0252947 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Han Na Suh Young Kyu Kim Ju Young Lee Goo-Hwa Kang Jeong Ho Hwang Dissect the immunity using cytokine profiling and NF-kB target gene analysis in systemic inflammatory minipig model.
description	Minipigs have remarkably similar physiology to humans, therefore, they it can be a good animal model for inflammation study. Thus, the conventional (serum chemistry, histopathology) and novel analytic tools [immune cell identification in tissue, cytokine level in peripheral blood mononuclear cells (PBMC) and serum, NF-kB target gene analysis in tissue] were applied to determine inflammation in Chicago Miniature Swine (CMS) minipig. Lipopolysaccharide (LPS)-induced acute systemic inflammation caused liver and kidney damage in serum chemistry and histopathology. Immunohistochemistry (IHC) also showed an increase of immune cell distribution in spleen and lung during inflammation. Moreover, NF-kB-target gene expression was upregulated in lung and kidney in acute inflammation and in heart, liver, and intestine in chronic inflammation. Cytokine mRNA was elevated in PBMC under acute inflammation along with elevated absolute cytokine levels in serum. Overall, LPS-mediated systemic inflammation affects the various organs, and can be detected by IHC of immune cells, gene analysis in PBMC, and measuring the absolute cytokine in serum along with conventional inflammation analytic tools.
format	article
author	Han Na Suh Young Kyu Kim Ju Young Lee Goo-Hwa Kang Jeong Ho Hwang
author_facet	Han Na Suh Young Kyu Kim Ju Young Lee Goo-Hwa Kang Jeong Ho Hwang
author_sort	Han Na Suh
title	Dissect the immunity using cytokine profiling and NF-kB target gene analysis in systemic inflammatory minipig model.
title_short	Dissect the immunity using cytokine profiling and NF-kB target gene analysis in systemic inflammatory minipig model.
title_full	Dissect the immunity using cytokine profiling and NF-kB target gene analysis in systemic inflammatory minipig model.
title_fullStr	Dissect the immunity using cytokine profiling and NF-kB target gene analysis in systemic inflammatory minipig model.
title_full_unstemmed	Dissect the immunity using cytokine profiling and NF-kB target gene analysis in systemic inflammatory minipig model.
title_sort	dissect the immunity using cytokine profiling and nf-kb target gene analysis in systemic inflammatory minipig model.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/7d6e686d8924434995ec7347605bf324
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Dissect the immunity using cytokine profiling and NF-kB target gene analysis in systemic inflammatory minipig model.

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