A highly sensitive biosensor based on Au NPs/rGO-PAMAM-Fc nanomaterials for detection of cholesterol

Jianmeng Zhu,1 Zhilu Ye,1 Xiaoying Fan,1 Hongqin Wang,1 Zhen Wang,1,2 Bingyu Chen1,2 1Department of Clinical Laboratory, Chun’an First People’s Hospital (Zhejiang Provincial People’s Hospital Chun’an Branch), Hangzhou, Zhejiang, China; 2Department of Blood Tr...

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Autores principales:	Zhu J, Ye Z, Fan X, Wang H, Wang Z, Chen B
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Biosensor Graphene Polyamide-amine Ferrocene Cholesterol Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7d728aa1a24349529c498a3f9c119fe6
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spelling	oai:doaj.org-article:7d728aa1a24349529c498a3f9c119fe62021-12-02T04:16:33ZA highly sensitive biosensor based on Au NPs/rGO-PAMAM-Fc nanomaterials for detection of cholesterol1178-2013https://doaj.org/article/7d728aa1a24349529c498a3f9c119fe62019-01-01T00:00:00Zhttps://www.dovepress.com/a-highly-sensitive-biosensor-based-on-au-npsrgo-pamam-fc-nanomaterials-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jianmeng Zhu,1 Zhilu Ye,1 Xiaoying Fan,1 Hongqin Wang,1 Zhen Wang,1,2 Bingyu Chen1,2 1Department of Clinical Laboratory, Chun’an First People’s Hospital (Zhejiang Provincial People’s Hospital Chun’an Branch), Hangzhou, Zhejiang, China; 2Department of Blood Transfusion, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, China Objective: This study aimed to construct a biosensor using Au nanoparticles (Au NPs) and reduced graphene-polyamide-amine-ferrocene (rGO-PAMAM-Fc) nanomaterials designed for rapid and sensitive detection of cholesterol.Materials and methods: In this study, a highly sensitive biosensor based on Au NPs/rGO-PAMAM-Fc nanomaterials was manufactured for detection of cholesterol. The rGO-PAMAM-Fc and Au NPs were modified on the surface of the electrode and then coated with cholesterol oxidase (ChOx) and cholesterol esterase (ChEt) to develop the ChOx&ChEt/Au NPs/rGO-PAMAM-Fc biosensor.Results: The capability of rGO-PAMAM-Fc nanomaterials in fabricating a more efficient biosensor was validated through stability, selectivity and reproducibility checks. Under optimal conditions, the newly developed biosensor showed a linear relationship with logarithm of cholesterol concentration from 0.0004 to 15.36 mM (R2=0.9986), and a low detection limit of 2 nM was obtained at the signal/noise ratio of 3.Conclusion: The ChOx&ChEt/Au NPs/rGO-PAMAM-Fc biosensor was successfully applied for the measurement of cholesterol in human serum, which implies that the biosensor has a potential application in clinical diagnostics. Keywords: diagnostics, graphene, polyamide-amine, ferrocene, Au nanoparticlesZhu JYe ZFan XWang HWang ZChen BDove Medical PressarticleBiosensorGraphenePolyamide-amineFerroceneCholesterolMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 835-849 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biosensor Graphene Polyamide-amine Ferrocene Cholesterol Medicine (General) R5-920
spellingShingle	Biosensor Graphene Polyamide-amine Ferrocene Cholesterol Medicine (General) R5-920 Zhu J Ye Z Fan X Wang H Wang Z Chen B A highly sensitive biosensor based on Au NPs/rGO-PAMAM-Fc nanomaterials for detection of cholesterol
description	Jianmeng Zhu,1 Zhilu Ye,1 Xiaoying Fan,1 Hongqin Wang,1 Zhen Wang,1,2 Bingyu Chen1,2 1Department of Clinical Laboratory, Chun’an First People’s Hospital (Zhejiang Provincial People’s Hospital Chun’an Branch), Hangzhou, Zhejiang, China; 2Department of Blood Transfusion, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, China Objective: This study aimed to construct a biosensor using Au nanoparticles (Au NPs) and reduced graphene-polyamide-amine-ferrocene (rGO-PAMAM-Fc) nanomaterials designed for rapid and sensitive detection of cholesterol.Materials and methods: In this study, a highly sensitive biosensor based on Au NPs/rGO-PAMAM-Fc nanomaterials was manufactured for detection of cholesterol. The rGO-PAMAM-Fc and Au NPs were modified on the surface of the electrode and then coated with cholesterol oxidase (ChOx) and cholesterol esterase (ChEt) to develop the ChOx&ChEt/Au NPs/rGO-PAMAM-Fc biosensor.Results: The capability of rGO-PAMAM-Fc nanomaterials in fabricating a more efficient biosensor was validated through stability, selectivity and reproducibility checks. Under optimal conditions, the newly developed biosensor showed a linear relationship with logarithm of cholesterol concentration from 0.0004 to 15.36 mM (R2=0.9986), and a low detection limit of 2 nM was obtained at the signal/noise ratio of 3.Conclusion: The ChOx&ChEt/Au NPs/rGO-PAMAM-Fc biosensor was successfully applied for the measurement of cholesterol in human serum, which implies that the biosensor has a potential application in clinical diagnostics. Keywords: diagnostics, graphene, polyamide-amine, ferrocene, Au nanoparticles
format	article
author	Zhu J Ye Z Fan X Wang H Wang Z Chen B
author_facet	Zhu J Ye Z Fan X Wang H Wang Z Chen B
author_sort	Zhu J
title	A highly sensitive biosensor based on Au NPs/rGO-PAMAM-Fc nanomaterials for detection of cholesterol
title_short	A highly sensitive biosensor based on Au NPs/rGO-PAMAM-Fc nanomaterials for detection of cholesterol
title_full	A highly sensitive biosensor based on Au NPs/rGO-PAMAM-Fc nanomaterials for detection of cholesterol
title_fullStr	A highly sensitive biosensor based on Au NPs/rGO-PAMAM-Fc nanomaterials for detection of cholesterol
title_full_unstemmed	A highly sensitive biosensor based on Au NPs/rGO-PAMAM-Fc nanomaterials for detection of cholesterol
title_sort	highly sensitive biosensor based on au nps/rgo-pamam-fc nanomaterials for detection of cholesterol
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/7d728aa1a24349529c498a3f9c119fe6
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A highly sensitive biosensor based on Au NPs/rGO-PAMAM-Fc nanomaterials for detection of cholesterol

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