$Assesment of platelet antiaggregant activity of the ethanolic fraction obtained from the bark of Nectandra amazonum Nees.$
Código QR

Assesment of platelet antiaggregant activity of the ethanolic fraction obtained from the bark of Nectandra amazonum Nees.

Background: Some species of the Lauraceae family are known to produce secondary metabolites that have antiplatelet properties. Studies on the leaves of Nectandra amazonum Nees. have shown antiaggregant activity but the bark of this species has not been studied. Objectives: To assess the antiplatele...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jenaro Antonio ESPITIA CORREDOR, Luis Enrique CUCA SUÁREZ, Mario Francisco GUERRERO PABÓN
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2016
Materias:	Lauraceae platelet aggregation arachidonic acid adenosine diphosphate epinephrine collagen Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
Acceso en línea:	https://doaj.org/article/7d731e4cd77048a3815a87c3f2fd32c5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7d731e4cd77048a3815a87c3f2fd32c5
record_format	dspace
spelling	oai:doaj.org-article:7d731e4cd77048a3815a87c3f2fd32c52021-11-19T04:09:46ZAssesment of platelet antiaggregant activity of the ethanolic fraction obtained from the bark of Nectandra amazonum Nees.0121-40042145-2660https://doaj.org/article/7d731e4cd77048a3815a87c3f2fd32c52016-09-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/vitae/article/view/24424https://doaj.org/toc/0121-4004https://doaj.org/toc/2145-2660 Background: Some species of the Lauraceae family are known to produce secondary metabolites that have antiplatelet properties. Studies on the leaves of Nectandra amazonum Nees. have shown antiaggregant activity but the bark of this species has not been studied. Objectives: To assess the antiplatelet effect of the ethanolic fraction obtained from the bark of Nectandra amazonum Nees. [N.V. “laurel amarillo”, Lauraceae] applying the “Born” turbidimetric method. Methods: The screening test compared the effects of a fraction of N. amazonum (0.1 mg/mL), acetylsalicylic acid (ASA, 0.5 mM, as reference standard) and dimethylsulphoxide (DMSO, 0.1%, as control) on human platelets stimulated with adenosine diphosphate (ADP, 2 µM), epinephrine (EPI, 1 uM (one micromolar)), collagen (COLL, 1 µg/mL) and arachidonic acid (AA, 0.2 mg/mL). Subsequently, the study focused on determining the antiaggregant potency of the N. amazonum fraction through concentration - response curves (from 1 µg/mL to 0.4 mg/mL), obtaining pIC50 (-log IC50) values against the platelet agonists. Results: Control platelets attained the highest percentage values of aggregation (96% AA, 89% EPI, 85% COLL, and 77% ADP). The N. amazonum fraction significantly reduced the aggregation effects (6% AA, 45% EPI, 10% COLL, 21% ADP), with values close to those obtained with ASA (17% AA, 21% EPI, 10% COLL, 20% ADP). According to concentration - response curves, the pIC50 values of the ethanolic fraction indicated the following order of potency: AA, 4.90 > ADP, 4.51 > COLL, 4.33 > EPI, 3.85. Conclusions: These results suggest that the N. amazonum Nees. ethanolic fraction elicited antiplatelet effects mainly related to the inhibition of the arachidonic acid pathway. Jenaro Antonio ESPITIA CORREDORLuis Enrique CUCA SUÁREZMario Francisco GUERRERO PABÓNUniversidad de AntioquiaarticleLauraceaeplatelet aggregationarachidonic acidadenosine diphosphateepinephrinecollagenFood processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 23, Iss 2 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Lauraceae platelet aggregation arachidonic acid adenosine diphosphate epinephrine collagen Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
spellingShingle	Lauraceae platelet aggregation arachidonic acid adenosine diphosphate epinephrine collagen Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 Jenaro Antonio ESPITIA CORREDOR Luis Enrique CUCA SUÁREZ Mario Francisco GUERRERO PABÓN Assesment of platelet antiaggregant activity of the ethanolic fraction obtained from the bark of Nectandra amazonum Nees.
description	Background: Some species of the Lauraceae family are known to produce secondary metabolites that have antiplatelet properties. Studies on the leaves of Nectandra amazonum Nees. have shown antiaggregant activity but the bark of this species has not been studied. Objectives: To assess the antiplatelet effect of the ethanolic fraction obtained from the bark of Nectandra amazonum Nees. [N.V. “laurel amarillo”, Lauraceae] applying the “Born” turbidimetric method. Methods: The screening test compared the effects of a fraction of N. amazonum (0.1 mg/mL), acetylsalicylic acid (ASA, 0.5 mM, as reference standard) and dimethylsulphoxide (DMSO, 0.1%, as control) on human platelets stimulated with adenosine diphosphate (ADP, 2 µM), epinephrine (EPI, 1 uM (one micromolar)), collagen (COLL, 1 µg/mL) and arachidonic acid (AA, 0.2 mg/mL). Subsequently, the study focused on determining the antiaggregant potency of the N. amazonum fraction through concentration - response curves (from 1 µg/mL to 0.4 mg/mL), obtaining pIC50 (-log IC50) values against the platelet agonists. Results: Control platelets attained the highest percentage values of aggregation (96% AA, 89% EPI, 85% COLL, and 77% ADP). The N. amazonum fraction significantly reduced the aggregation effects (6% AA, 45% EPI, 10% COLL, 21% ADP), with values close to those obtained with ASA (17% AA, 21% EPI, 10% COLL, 20% ADP). According to concentration - response curves, the pIC50 values of the ethanolic fraction indicated the following order of potency: AA, 4.90 > ADP, 4.51 > COLL, 4.33 > EPI, 3.85. Conclusions: These results suggest that the N. amazonum Nees. ethanolic fraction elicited antiplatelet effects mainly related to the inhibition of the arachidonic acid pathway.
format	article
author	Jenaro Antonio ESPITIA CORREDOR Luis Enrique CUCA SUÁREZ Mario Francisco GUERRERO PABÓN
author_facet	Jenaro Antonio ESPITIA CORREDOR Luis Enrique CUCA SUÁREZ Mario Francisco GUERRERO PABÓN
author_sort	Jenaro Antonio ESPITIA CORREDOR
title	Assesment of platelet antiaggregant activity of the ethanolic fraction obtained from the bark of Nectandra amazonum Nees.
title_short	Assesment of platelet antiaggregant activity of the ethanolic fraction obtained from the bark of Nectandra amazonum Nees.
title_full	Assesment of platelet antiaggregant activity of the ethanolic fraction obtained from the bark of Nectandra amazonum Nees.
title_fullStr	Assesment of platelet antiaggregant activity of the ethanolic fraction obtained from the bark of Nectandra amazonum Nees.
title_full_unstemmed	Assesment of platelet antiaggregant activity of the ethanolic fraction obtained from the bark of Nectandra amazonum Nees.
title_sort	assesment of platelet antiaggregant activity of the ethanolic fraction obtained from the bark of nectandra amazonum nees.
publisher	Universidad de Antioquia
publishDate	2016
url	https://doaj.org/article/7d731e4cd77048a3815a87c3f2fd32c5
work_keys_str_mv	AT jenaroantonioespitiacorredor assesmentofplateletantiaggregantactivityoftheethanolicfractionobtainedfromthebarkofnectandraamazonumnees AT luisenriquecucasuarez assesmentofplateletantiaggregantactivityoftheethanolicfractionobtainedfromthebarkofnectandraamazonumnees AT mariofranciscoguerreropabon assesmentofplateletantiaggregantactivityoftheethanolicfractionobtainedfromthebarkofnectandraamazonumnees
_version_	1718420503374331904

Assesment of platelet antiaggregant activity of the ethanolic fraction obtained from the bark of Nectandra amazonum Nees.

Ejemplares similares