Rat model of an autologous cancellous bone graft

Abstract Autologous cancellous bone (ACB) grafting is the “gold standard” treatment for delayed bone union. However, small animal models for such grafts are lacking. Here, we developed an ACB graft rat model. Anatomical information regarding the iliac structure was recorded from five rat cadavers (1...

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Autores principales:	Tomo Hamada, Hidenori Matsubara, Toshifumi Hikichi, Kanu Shimokawa, Hiroyuki Tsuchiya
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7d73cb2fcba8464a8c5f6fe378d163cf
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spelling	oai:doaj.org-article:7d73cb2fcba8464a8c5f6fe378d163cf2021-12-02T19:12:31ZRat model of an autologous cancellous bone graft10.1038/s41598-021-97573-02045-2322https://doaj.org/article/7d73cb2fcba8464a8c5f6fe378d163cf2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97573-0https://doaj.org/toc/2045-2322Abstract Autologous cancellous bone (ACB) grafting is the “gold standard” treatment for delayed bone union. However, small animal models for such grafts are lacking. Here, we developed an ACB graft rat model. Anatomical information regarding the iliac structure was recorded from five rat cadavers (10 ilia). Additionally, 5 and 25 rats were used as controls and ACB graft models, respectively. A defect was created in rat femurs and filled with ACB. Post-graft neo-osteogenic potential was assessed by radiographic evaluation and histological analysis. Iliac bone harvesting yielded the maximum amount of cancellous bone with minimal invasiveness, considering the position of parailiac nerves and vessels. The mean volume of cancellous bone per rat separated from the cortical bone was 73.8 ± 5.5 mm3. Bone union was evident in all ACB graft groups at 8 weeks, and new bone volume significantly increased every 2 weeks (P < 0.001). Histological analysis demonstrated the ability of ACB grafts to act as a scaffold and promote bone union in the defect. In conclusion, we established a stable rat model of ACB grafts by harvesting the iliac bone. This model can aid in investigating ACB grafts and development of novel therapies for bone injury.Tomo HamadaHidenori MatsubaraToshifumi HikichiKanu ShimokawaHiroyuki TsuchiyaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tomo Hamada Hidenori Matsubara Toshifumi Hikichi Kanu Shimokawa Hiroyuki Tsuchiya Rat model of an autologous cancellous bone graft
description	Abstract Autologous cancellous bone (ACB) grafting is the “gold standard” treatment for delayed bone union. However, small animal models for such grafts are lacking. Here, we developed an ACB graft rat model. Anatomical information regarding the iliac structure was recorded from five rat cadavers (10 ilia). Additionally, 5 and 25 rats were used as controls and ACB graft models, respectively. A defect was created in rat femurs and filled with ACB. Post-graft neo-osteogenic potential was assessed by radiographic evaluation and histological analysis. Iliac bone harvesting yielded the maximum amount of cancellous bone with minimal invasiveness, considering the position of parailiac nerves and vessels. The mean volume of cancellous bone per rat separated from the cortical bone was 73.8 ± 5.5 mm3. Bone union was evident in all ACB graft groups at 8 weeks, and new bone volume significantly increased every 2 weeks (P < 0.001). Histological analysis demonstrated the ability of ACB grafts to act as a scaffold and promote bone union in the defect. In conclusion, we established a stable rat model of ACB grafts by harvesting the iliac bone. This model can aid in investigating ACB grafts and development of novel therapies for bone injury.
format	article
author	Tomo Hamada Hidenori Matsubara Toshifumi Hikichi Kanu Shimokawa Hiroyuki Tsuchiya
author_facet	Tomo Hamada Hidenori Matsubara Toshifumi Hikichi Kanu Shimokawa Hiroyuki Tsuchiya
author_sort	Tomo Hamada
title	Rat model of an autologous cancellous bone graft
title_short	Rat model of an autologous cancellous bone graft
title_full	Rat model of an autologous cancellous bone graft
title_fullStr	Rat model of an autologous cancellous bone graft
title_full_unstemmed	Rat model of an autologous cancellous bone graft
title_sort	rat model of an autologous cancellous bone graft
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7d73cb2fcba8464a8c5f6fe378d163cf
work_keys_str_mv	AT tomohamada ratmodelofanautologouscancellousbonegraft AT hidenorimatsubara ratmodelofanautologouscancellousbonegraft AT toshifumihikichi ratmodelofanautologouscancellousbonegraft AT kanushimokawa ratmodelofanautologouscancellousbonegraft AT hiroyukitsuchiya ratmodelofanautologouscancellousbonegraft
_version_	1718377053050372096

Rat model of an autologous cancellous bone graft

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