Novel complex of HAT protein TIP60 and nuclear receptor PXR promotes cell migration and adhesion

Abstract PXR is a member of nuclear receptor superfamily and a well-characterized mediator of xenobiotic metabolism. The classical mode of PXR activation involves its binding to appropriate ligand and subsequent heterodimerization with its partner RXR. However, various factors such as post-translati...

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Autores principales:	Karishma Bakshi, B. Ranjitha, Shraddha Dubey, Jaisri Jagannadham, Bharti Jaiswal, Ashish Gupta
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7d754198b12f45f2b165e5658feeee64
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spelling	oai:doaj.org-article:7d754198b12f45f2b165e5658feeee642021-12-02T12:32:59ZNovel complex of HAT protein TIP60 and nuclear receptor PXR promotes cell migration and adhesion10.1038/s41598-017-03783-w2045-2322https://doaj.org/article/7d754198b12f45f2b165e5658feeee642017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03783-whttps://doaj.org/toc/2045-2322Abstract PXR is a member of nuclear receptor superfamily and a well-characterized mediator of xenobiotic metabolism. The classical mode of PXR activation involves its binding to appropriate ligand and subsequent heterodimerization with its partner RXR. However, various factors such as post-translational modifications and crosstalk with different cellular factors may also regulate the functional dynamics and behavior of PXR. In the present study, we have identified that TIP60, an essential lysine acetyltransferase protein interacts with unliganded PXR and together this complex promotes cell migration & adhesion. TIP60 utilizes its NR Box to interact with LBD region of PXR and acetylates PXR at lysine 170 to induce its intranuclear reorganization. Also, RXR is not required for TIP60-PXR complex formation and this complex does not induce ligand-dependent PXR target gene transactivation. Interestingly, we observed that PXR augments the catalytic activity of TIP60 for histones. This is the first report demonstrating the exclusive interaction of TIP60 with PXR and uncovers a potential role for the TIP60-PXR complex in cell migration and adhesion.Karishma BakshiB. RanjithaShraddha DubeyJaisri JagannadhamBharti JaiswalAshish GuptaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-16 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Karishma Bakshi B. Ranjitha Shraddha Dubey Jaisri Jagannadham Bharti Jaiswal Ashish Gupta Novel complex of HAT protein TIP60 and nuclear receptor PXR promotes cell migration and adhesion
description	Abstract PXR is a member of nuclear receptor superfamily and a well-characterized mediator of xenobiotic metabolism. The classical mode of PXR activation involves its binding to appropriate ligand and subsequent heterodimerization with its partner RXR. However, various factors such as post-translational modifications and crosstalk with different cellular factors may also regulate the functional dynamics and behavior of PXR. In the present study, we have identified that TIP60, an essential lysine acetyltransferase protein interacts with unliganded PXR and together this complex promotes cell migration & adhesion. TIP60 utilizes its NR Box to interact with LBD region of PXR and acetylates PXR at lysine 170 to induce its intranuclear reorganization. Also, RXR is not required for TIP60-PXR complex formation and this complex does not induce ligand-dependent PXR target gene transactivation. Interestingly, we observed that PXR augments the catalytic activity of TIP60 for histones. This is the first report demonstrating the exclusive interaction of TIP60 with PXR and uncovers a potential role for the TIP60-PXR complex in cell migration and adhesion.
format	article
author	Karishma Bakshi B. Ranjitha Shraddha Dubey Jaisri Jagannadham Bharti Jaiswal Ashish Gupta
author_facet	Karishma Bakshi B. Ranjitha Shraddha Dubey Jaisri Jagannadham Bharti Jaiswal Ashish Gupta
author_sort	Karishma Bakshi
title	Novel complex of HAT protein TIP60 and nuclear receptor PXR promotes cell migration and adhesion
title_short	Novel complex of HAT protein TIP60 and nuclear receptor PXR promotes cell migration and adhesion
title_full	Novel complex of HAT protein TIP60 and nuclear receptor PXR promotes cell migration and adhesion
title_fullStr	Novel complex of HAT protein TIP60 and nuclear receptor PXR promotes cell migration and adhesion
title_full_unstemmed	Novel complex of HAT protein TIP60 and nuclear receptor PXR promotes cell migration and adhesion
title_sort	novel complex of hat protein tip60 and nuclear receptor pxr promotes cell migration and adhesion
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7d754198b12f45f2b165e5658feeee64
work_keys_str_mv	AT karishmabakshi novelcomplexofhatproteintip60andnuclearreceptorpxrpromotescellmigrationandadhesion AT branjitha novelcomplexofhatproteintip60andnuclearreceptorpxrpromotescellmigrationandadhesion AT shraddhadubey novelcomplexofhatproteintip60andnuclearreceptorpxrpromotescellmigrationandadhesion AT jaisrijagannadham novelcomplexofhatproteintip60andnuclearreceptorpxrpromotescellmigrationandadhesion AT bhartijaiswal novelcomplexofhatproteintip60andnuclearreceptorpxrpromotescellmigrationandadhesion AT ashishgupta novelcomplexofhatproteintip60andnuclearreceptorpxrpromotescellmigrationandadhesion
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Novel complex of HAT protein TIP60 and nuclear receptor PXR promotes cell migration and adhesion

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