Amniotic Membrane Transplantation for Persistent Epithelial Defects and Ulceration due to Pseudomonas Keratitis in a Rabbit Model

Abstract Purpose: The use of amniotic membrane has been suggested in the treatment of infectious keratitis for its intrinsic anti-infective properties probably mediated by its anti-inflammatory effects. The aim of this study was to investigate the effect of amniotic membrane transplantation (AMT) al...

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Autores principales:	Mohammad Mehdi Soltan Dallal, Farhad Nikkhahi, Seyed Mostafa Imeni, Saber Molaei, Seyed Kazem Hosseini, Zohreh Kalafi, Sara Sharifi Yazdi, Hedroosha Molla Agha Mirzaei
Formato:	article
Lenguaje:	EN
Publicado:	Knowledge E 2021
Materias:	ciprofloxacin human amniotic membrane keratitis pseudomonas aeruginosa rabbit Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/7d7f2d91b1fd43bd86a451037a71b539
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spelling	oai:doaj.org-article:7d7f2d91b1fd43bd86a451037a71b5392021-11-09T08:05:35ZAmniotic Membrane Transplantation for Persistent Epithelial Defects and Ulceration due to Pseudomonas Keratitis in a Rabbit Model2008-20102008-322X10.18502/jovr.v16i4.9744https://doaj.org/article/7d7f2d91b1fd43bd86a451037a71b5392021-10-01T00:00:00Zhttps://doi.org/10.18502/jovr.v16i4.9744https://doaj.org/toc/2008-2010https://doaj.org/toc/2008-322XAbstract Purpose: The use of amniotic membrane has been suggested in the treatment of infectious keratitis for its intrinsic anti-infective properties probably mediated by its anti-inflammatory effects. The aim of this study was to investigate the effect of amniotic membrane transplantation (AMT) along with ciprofloxacin to cure the primary stages of Pseudomonas keratitis. Methods: In total, 28 rabbits were selected and divided in four groups as follows: group 1 as control, group 2 with amniotic membrane, group 3 with ciprofloxacin, and group 4 with amniotic membrane combined with ciprofloxacin. About 0.05 cc suspension of Pseudomonas aeruginosa, 27853 ATCC was injected into corneal stroma. Results: The results showed groups of AMT, AMT + ciprofloxacin, and ciprofloxacin had 0% perforation while the control group had 85.6%. Average infiltration of 5.5 mm was observed in ciprofloxacin group, 5 mm in AMT + ciprofloxacin group, 24 mm in AMT group, and finally 23.75 mm for control. Amniotic membrane showed to be effective in prevention of cornea perforation as well as remission of Pseudomonas keratitis. There was no significant difference between ciprofloxacin groups in comparison with ciprofloxacin + AMT group. However, regarding the anti-inflammatory effect, the process of improvement of inflammation in ciprofloxacin + AMT group was faster. Conclusion: Transplantation of amniotic membrane in the primary stages of Pseudomonas keratitis treatment remarkably prevents the disease and it can be used to control its process.Mohammad Mehdi Soltan DallalFarhad NikkhahiSeyed Mostafa ImeniSaber MolaeiSeyed Kazem HosseiniZohreh KalafiSara Sharifi YazdiHedroosha Molla Agha MirzaeiKnowledge Earticleciprofloxacinhuman amniotic membranekeratitispseudomonas aeruginosarabbitOphthalmologyRE1-994ENJournal of Ophthalmic & Vision Research, Vol 16, Iss 4, Pp 552-557 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ciprofloxacin human amniotic membrane keratitis pseudomonas aeruginosa rabbit Ophthalmology RE1-994
spellingShingle	ciprofloxacin human amniotic membrane keratitis pseudomonas aeruginosa rabbit Ophthalmology RE1-994 Mohammad Mehdi Soltan Dallal Farhad Nikkhahi Seyed Mostafa Imeni Saber Molaei Seyed Kazem Hosseini Zohreh Kalafi Sara Sharifi Yazdi Hedroosha Molla Agha Mirzaei Amniotic Membrane Transplantation for Persistent Epithelial Defects and Ulceration due to Pseudomonas Keratitis in a Rabbit Model
description	Abstract Purpose: The use of amniotic membrane has been suggested in the treatment of infectious keratitis for its intrinsic anti-infective properties probably mediated by its anti-inflammatory effects. The aim of this study was to investigate the effect of amniotic membrane transplantation (AMT) along with ciprofloxacin to cure the primary stages of Pseudomonas keratitis. Methods: In total, 28 rabbits were selected and divided in four groups as follows: group 1 as control, group 2 with amniotic membrane, group 3 with ciprofloxacin, and group 4 with amniotic membrane combined with ciprofloxacin. About 0.05 cc suspension of Pseudomonas aeruginosa, 27853 ATCC was injected into corneal stroma. Results: The results showed groups of AMT, AMT + ciprofloxacin, and ciprofloxacin had 0% perforation while the control group had 85.6%. Average infiltration of 5.5 mm was observed in ciprofloxacin group, 5 mm in AMT + ciprofloxacin group, 24 mm in AMT group, and finally 23.75 mm for control. Amniotic membrane showed to be effective in prevention of cornea perforation as well as remission of Pseudomonas keratitis. There was no significant difference between ciprofloxacin groups in comparison with ciprofloxacin + AMT group. However, regarding the anti-inflammatory effect, the process of improvement of inflammation in ciprofloxacin + AMT group was faster. Conclusion: Transplantation of amniotic membrane in the primary stages of Pseudomonas keratitis treatment remarkably prevents the disease and it can be used to control its process.
format	article
author	Mohammad Mehdi Soltan Dallal Farhad Nikkhahi Seyed Mostafa Imeni Saber Molaei Seyed Kazem Hosseini Zohreh Kalafi Sara Sharifi Yazdi Hedroosha Molla Agha Mirzaei
author_facet	Mohammad Mehdi Soltan Dallal Farhad Nikkhahi Seyed Mostafa Imeni Saber Molaei Seyed Kazem Hosseini Zohreh Kalafi Sara Sharifi Yazdi Hedroosha Molla Agha Mirzaei
author_sort	Mohammad Mehdi Soltan Dallal
title	Amniotic Membrane Transplantation for Persistent Epithelial Defects and Ulceration due to Pseudomonas Keratitis in a Rabbit Model
title_short	Amniotic Membrane Transplantation for Persistent Epithelial Defects and Ulceration due to Pseudomonas Keratitis in a Rabbit Model
title_full	Amniotic Membrane Transplantation for Persistent Epithelial Defects and Ulceration due to Pseudomonas Keratitis in a Rabbit Model
title_fullStr	Amniotic Membrane Transplantation for Persistent Epithelial Defects and Ulceration due to Pseudomonas Keratitis in a Rabbit Model
title_full_unstemmed	Amniotic Membrane Transplantation for Persistent Epithelial Defects and Ulceration due to Pseudomonas Keratitis in a Rabbit Model
title_sort	amniotic membrane transplantation for persistent epithelial defects and ulceration due to pseudomonas keratitis in a rabbit model
publisher	Knowledge E
publishDate	2021
url	https://doaj.org/article/7d7f2d91b1fd43bd86a451037a71b539
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Amniotic Membrane Transplantation for Persistent Epithelial Defects and Ulceration due to Pseudomonas Keratitis in a Rabbit Model

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