In vivo MRI tracking of iron oxide nanoparticle-labeled human mesenchymal stem cells in limb ischemia

Xiang-Xiang Li,1,2,* Kang-An Li,3,* Jin-Bao Qin,1,2 Kai-Chuang Ye,1,2 Xin-Rui Yang,1,2 Wei-Min Li,1,2 Qing-Song Xie,4 Mi-Er Jiang,1,2 Gui-Xiang Zhang,3 Xin-Wu Lu1,21Department of Vascular Surgery, Shanghai Ninth People&#39;s Hospital Affiliated to Shanghai Jiao Tong University, School of Med...

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Autores principales:	Li XX, Li KA, Qin JB, Ye KC, Yang XR, Li WM, Xie QS, Jiang ME, Zhang GX, Lu XW.
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7d8658a5da494e9ea04b790744f477da
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spelling	oai:doaj.org-article:7d8658a5da494e9ea04b790744f477da2021-12-02T09:08:32ZIn vivo MRI tracking of iron oxide nanoparticle-labeled human mesenchymal stem cells in limb ischemia1176-91141178-2013https://doaj.org/article/7d8658a5da494e9ea04b790744f477da2013-03-01T00:00:00Zhttp://www.dovepress.com/in-vivo-mri-tracking-of-iron-oxide-nanoparticle-labeled-human-mesenchy-a12439https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Xiang-Xiang Li,1,2,* Kang-An Li,3,* Jin-Bao Qin,1,2 Kai-Chuang Ye,1,2 Xin-Rui Yang,1,2 Wei-Min Li,1,2 Qing-Song Xie,4 Mi-Er Jiang,1,2 Gui-Xiang Zhang,3 Xin-Wu Lu1,21Department of Vascular Surgery, Shanghai Ninth People&#39;s Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, 2Vascular Center, Shanghai JiaoTong University, 3Department of Radiology, Shanghai First People&#39;s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People&#39;s Republic of China; 4Department of Neurosurgery, Cixi Municipal People&#39;s Hospital, Zhejiang Province, People&#39;s Republic of China *These authors contributed equally to this work Background: Stem cell transplantation has been investigated for repairing damaged tissues in various injury models. Monitoring the safety and fate of transplanted cells using noninvasive methods is important to advance this technique into clinical applications. Methods: In this study, lower-limb ischemia models were generated in nude mice by femoral artery ligation. As negative-contrast agents, positively charged magnetic iron oxide nanoparticles (aminopropyltriethoxysilane-coated Fe2O3) were investigated in terms of in vitro labeling efficiency, effects on human mesenchymal stromal cell (hMSC) proliferation, and in vivo magnetic resonance imaging (MRI) visualization. Ultimately, the mice were sacrificed for histological analysis three weeks after transplantation. Results: With efficient labeling, aminopropyltriethoxysilane-modified magnetic iron oxide nanoparticles (APTS-MNPs) did not significantly affect hMSC proliferation. In vivo, APTS-MNP-labeled hMSCs could be monitored by clinical 3 Tesla MRI for at least three weeks. Histological examination detected numerous migrated Prussian blue-positive cells, which was consistent with the magnetic resonance images. Some migrated Prussian blue-positive cells were positive for mature endothelial cell markers of von Willebrand factor and anti-human proliferating cell nuclear antigen. In the test groups, Prussian blue-positive nanoparticles, which could not be found in other organs, were detected in the spleen. Conclusion: APTS-MNPs could efficiently label hMSCs, and clinical 3 Tesla MRI could monitor the labeled stem cells in vivo, which may provide a new approach for the in vivo monitoring of implanted cells. Keywords: hind-limb ischemia, magnetic resonance imaging, iron oxide particles, stem cell implantLi XXLi KAQin JBYe KCYang XRLi WMXie QSJiang MEZhang GXLu XW.Dove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 1063-1073 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Li XX Li KA Qin JB Ye KC Yang XR Li WM Xie QS Jiang ME Zhang GX Lu XW. In vivo MRI tracking of iron oxide nanoparticle-labeled human mesenchymal stem cells in limb ischemia
description	Xiang-Xiang Li,1,2,* Kang-An Li,3,* Jin-Bao Qin,1,2 Kai-Chuang Ye,1,2 Xin-Rui Yang,1,2 Wei-Min Li,1,2 Qing-Song Xie,4 Mi-Er Jiang,1,2 Gui-Xiang Zhang,3 Xin-Wu Lu1,21Department of Vascular Surgery, Shanghai Ninth People&#39;s Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, 2Vascular Center, Shanghai JiaoTong University, 3Department of Radiology, Shanghai First People&#39;s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People&#39;s Republic of China; 4Department of Neurosurgery, Cixi Municipal People&#39;s Hospital, Zhejiang Province, People&#39;s Republic of China *These authors contributed equally to this work Background: Stem cell transplantation has been investigated for repairing damaged tissues in various injury models. Monitoring the safety and fate of transplanted cells using noninvasive methods is important to advance this technique into clinical applications. Methods: In this study, lower-limb ischemia models were generated in nude mice by femoral artery ligation. As negative-contrast agents, positively charged magnetic iron oxide nanoparticles (aminopropyltriethoxysilane-coated Fe2O3) were investigated in terms of in vitro labeling efficiency, effects on human mesenchymal stromal cell (hMSC) proliferation, and in vivo magnetic resonance imaging (MRI) visualization. Ultimately, the mice were sacrificed for histological analysis three weeks after transplantation. Results: With efficient labeling, aminopropyltriethoxysilane-modified magnetic iron oxide nanoparticles (APTS-MNPs) did not significantly affect hMSC proliferation. In vivo, APTS-MNP-labeled hMSCs could be monitored by clinical 3 Tesla MRI for at least three weeks. Histological examination detected numerous migrated Prussian blue-positive cells, which was consistent with the magnetic resonance images. Some migrated Prussian blue-positive cells were positive for mature endothelial cell markers of von Willebrand factor and anti-human proliferating cell nuclear antigen. In the test groups, Prussian blue-positive nanoparticles, which could not be found in other organs, were detected in the spleen. Conclusion: APTS-MNPs could efficiently label hMSCs, and clinical 3 Tesla MRI could monitor the labeled stem cells in vivo, which may provide a new approach for the in vivo monitoring of implanted cells. Keywords: hind-limb ischemia, magnetic resonance imaging, iron oxide particles, stem cell implant
format	article
author	Li XX Li KA Qin JB Ye KC Yang XR Li WM Xie QS Jiang ME Zhang GX Lu XW.
author_facet	Li XX Li KA Qin JB Ye KC Yang XR Li WM Xie QS Jiang ME Zhang GX Lu XW.
author_sort	Li XX
title	In vivo MRI tracking of iron oxide nanoparticle-labeled human mesenchymal stem cells in limb ischemia
title_short	In vivo MRI tracking of iron oxide nanoparticle-labeled human mesenchymal stem cells in limb ischemia
title_full	In vivo MRI tracking of iron oxide nanoparticle-labeled human mesenchymal stem cells in limb ischemia
title_fullStr	In vivo MRI tracking of iron oxide nanoparticle-labeled human mesenchymal stem cells in limb ischemia
title_full_unstemmed	In vivo MRI tracking of iron oxide nanoparticle-labeled human mesenchymal stem cells in limb ischemia
title_sort	in vivo mri tracking of iron oxide nanoparticle-labeled human mesenchymal stem cells in limb ischemia
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/7d8658a5da494e9ea04b790744f477da
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In vivo MRI tracking of iron oxide nanoparticle-labeled human mesenchymal stem cells in limb ischemia

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