Chondroitin Sulfate and Fucosylated Chondroitin Sulfate as Stimulators of Hematopoiesis in Cyclophosphamide-Induced Mice

The immunosuppression and inhibition of hematopoiesis are considered to be reasons for the development of complications after intensive chemotherapy and allogeneic hematopoietic stem cell transplantation. Chondroitin sulfate (<b>CS</b>), isolated from the fish <i>Salmo salar</i&...

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Autores principales:	Nadezhda E. Ustyuzhanina, Natalia Yu. Anisimova, Maria I. Bilan, Fedor V. Donenko, Galina E. Morozevich, Dmitriy V. Yashunskiy, Anatolii I. Usov, Nara G. Siminyan, Kirill I. Kirgisov, Svetlana R. Varfolomeeva, Mikhail V. Kiselevskiy, Nikolay E. Nifantiev
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	graft versus host disease granulocyte colony-stimulating factor chondroitin sulfate fucosylated chondroitin sulfate hematopoiesis immunosuppression Medicine R Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/7d890cf1cf0d4914a77618a120abbfa9
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spelling	oai:doaj.org-article:7d890cf1cf0d4914a77618a120abbfa92021-11-25T18:39:07ZChondroitin Sulfate and Fucosylated Chondroitin Sulfate as Stimulators of Hematopoiesis in Cyclophosphamide-Induced Mice10.3390/ph141110741424-8247https://doaj.org/article/7d890cf1cf0d4914a77618a120abbfa92021-10-01T00:00:00Zhttps://www.mdpi.com/1424-8247/14/11/1074https://doaj.org/toc/1424-8247The immunosuppression and inhibition of hematopoiesis are considered to be reasons for the development of complications after intensive chemotherapy and allogeneic hematopoietic stem cell transplantation. Chondroitin sulfate (<b>CS</b>), isolated from the fish <i>Salmo salar</i>, and fucosylated chondroitin sulfate (<b>FCS</b>), isolated from the sea cucumber <i>Apostichopus japonicus</i>, were studied for their roles as stimulators of hematopoiesis in a model of cyclophosphamide-induced immunosuppression in mice. The recombinant protein r G-CSF was applied as a reference. The studied polysaccharides were shown to stimulate the release of white and red blood cells, as well as platelets from bone marrow in immunosuppressed mice, while r G-CSF was only responsible for the significant increase in the level of leucocytes. The analysis of different populations of leucocytes in blood indicated that r G-CSF mainly stimulated the production of neutrophils, whereas in the cases of the studied saccharides, increases in the levels of monocytes, lymphocytes and neutrophils were observed. The normalization of the level of the pro-inflammatory cytokine IL-6 in the serum and the recovery of cell populations in the spleen were observed in immunosuppressed mice following treatment with the polysaccharides. An increase in the proliferative activity of hematopoietic cells CD34(+)CD45(+) was observed following ex vivo polysaccharide exposure. Further study on related oligosaccharides regarding their potential as promising drugs in the complex prophylaxis and therapy of hematopoiesis inhibition after intensive chemotherapy and allogeneic hematopoietic stem cell transplantation seems to be warranted.Nadezhda E. UstyuzhaninaNatalia Yu. AnisimovaMaria I. BilanFedor V. DonenkoGalina E. MorozevichDmitriy V. YashunskiyAnatolii I. UsovNara G. SiminyanKirill I. KirgisovSvetlana R. VarfolomeevaMikhail V. KiselevskiyNikolay E. NifantievMDPI AGarticlegraft versus host diseasegranulocyte colony-stimulating factorchondroitin sulfatefucosylated chondroitin sulfatehematopoiesisimmunosuppressionMedicineRPharmacy and materia medicaRS1-441ENPharmaceuticals, Vol 14, Iss 1074, p 1074 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	graft versus host disease granulocyte colony-stimulating factor chondroitin sulfate fucosylated chondroitin sulfate hematopoiesis immunosuppression Medicine R Pharmacy and materia medica RS1-441
spellingShingle	graft versus host disease granulocyte colony-stimulating factor chondroitin sulfate fucosylated chondroitin sulfate hematopoiesis immunosuppression Medicine R Pharmacy and materia medica RS1-441 Nadezhda E. Ustyuzhanina Natalia Yu. Anisimova Maria I. Bilan Fedor V. Donenko Galina E. Morozevich Dmitriy V. Yashunskiy Anatolii I. Usov Nara G. Siminyan Kirill I. Kirgisov Svetlana R. Varfolomeeva Mikhail V. Kiselevskiy Nikolay E. Nifantiev Chondroitin Sulfate and Fucosylated Chondroitin Sulfate as Stimulators of Hematopoiesis in Cyclophosphamide-Induced Mice
description	The immunosuppression and inhibition of hematopoiesis are considered to be reasons for the development of complications after intensive chemotherapy and allogeneic hematopoietic stem cell transplantation. Chondroitin sulfate (<b>CS</b>), isolated from the fish <i>Salmo salar</i>, and fucosylated chondroitin sulfate (<b>FCS</b>), isolated from the sea cucumber <i>Apostichopus japonicus</i>, were studied for their roles as stimulators of hematopoiesis in a model of cyclophosphamide-induced immunosuppression in mice. The recombinant protein r G-CSF was applied as a reference. The studied polysaccharides were shown to stimulate the release of white and red blood cells, as well as platelets from bone marrow in immunosuppressed mice, while r G-CSF was only responsible for the significant increase in the level of leucocytes. The analysis of different populations of leucocytes in blood indicated that r G-CSF mainly stimulated the production of neutrophils, whereas in the cases of the studied saccharides, increases in the levels of monocytes, lymphocytes and neutrophils were observed. The normalization of the level of the pro-inflammatory cytokine IL-6 in the serum and the recovery of cell populations in the spleen were observed in immunosuppressed mice following treatment with the polysaccharides. An increase in the proliferative activity of hematopoietic cells CD34(+)CD45(+) was observed following ex vivo polysaccharide exposure. Further study on related oligosaccharides regarding their potential as promising drugs in the complex prophylaxis and therapy of hematopoiesis inhibition after intensive chemotherapy and allogeneic hematopoietic stem cell transplantation seems to be warranted.
format	article
author	Nadezhda E. Ustyuzhanina Natalia Yu. Anisimova Maria I. Bilan Fedor V. Donenko Galina E. Morozevich Dmitriy V. Yashunskiy Anatolii I. Usov Nara G. Siminyan Kirill I. Kirgisov Svetlana R. Varfolomeeva Mikhail V. Kiselevskiy Nikolay E. Nifantiev
author_facet	Nadezhda E. Ustyuzhanina Natalia Yu. Anisimova Maria I. Bilan Fedor V. Donenko Galina E. Morozevich Dmitriy V. Yashunskiy Anatolii I. Usov Nara G. Siminyan Kirill I. Kirgisov Svetlana R. Varfolomeeva Mikhail V. Kiselevskiy Nikolay E. Nifantiev
author_sort	Nadezhda E. Ustyuzhanina
title	Chondroitin Sulfate and Fucosylated Chondroitin Sulfate as Stimulators of Hematopoiesis in Cyclophosphamide-Induced Mice
title_short	Chondroitin Sulfate and Fucosylated Chondroitin Sulfate as Stimulators of Hematopoiesis in Cyclophosphamide-Induced Mice
title_full	Chondroitin Sulfate and Fucosylated Chondroitin Sulfate as Stimulators of Hematopoiesis in Cyclophosphamide-Induced Mice
title_fullStr	Chondroitin Sulfate and Fucosylated Chondroitin Sulfate as Stimulators of Hematopoiesis in Cyclophosphamide-Induced Mice
title_full_unstemmed	Chondroitin Sulfate and Fucosylated Chondroitin Sulfate as Stimulators of Hematopoiesis in Cyclophosphamide-Induced Mice
title_sort	chondroitin sulfate and fucosylated chondroitin sulfate as stimulators of hematopoiesis in cyclophosphamide-induced mice
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7d890cf1cf0d4914a77618a120abbfa9
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Chondroitin Sulfate and Fucosylated Chondroitin Sulfate as Stimulators of Hematopoiesis in Cyclophosphamide-Induced Mice

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