Uniaxially fixed mechanical boundary condition elicits cellular alignment in collagen matrix with induction of osteogenesis

Abstract Osteocytes differentiated from osteoblasts play significant roles as mechanosensors in modulating the bone remodeling process. While the well-aligned osteocyte network along the trabeculae with slender cell processes perpendicular to the trabeculae surface is known to facilitate the sensing...

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Autores principales:	Jeonghyun Kim, Keiichi Ishikawa, Junko Sunaga, Taiji Adachi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7d8c2b39cd454626b0f95c11b79de3de
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spelling	oai:doaj.org-article:7d8c2b39cd454626b0f95c11b79de3de2021-12-02T16:55:46ZUniaxially fixed mechanical boundary condition elicits cellular alignment in collagen matrix with induction of osteogenesis10.1038/s41598-021-88505-z2045-2322https://doaj.org/article/7d8c2b39cd454626b0f95c11b79de3de2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88505-zhttps://doaj.org/toc/2045-2322Abstract Osteocytes differentiated from osteoblasts play significant roles as mechanosensors in modulating the bone remodeling process. While the well-aligned osteocyte network along the trabeculae with slender cell processes perpendicular to the trabeculae surface is known to facilitate the sensing of mechanical stimuli by cells and the intracellular communication in the bone matrix, the mechanisms underlying osteocyte network formation remains unclear. Here, we developed a novel in vitro collagen matrix system exerting a uniaxially-fixed mechanical boundary condition on which mouse osteoblast-like MC3T3-E1 cells were subcultured, evoking cellular alignment along the uniaxial boundary condition. Using a myosin II inhibitor, blebbistatin, we showed that the intracellular tension via contraction of actin fibers contributed to the cellular alignment under the influence of isometric matrix condition along the uniaxially-fixed mechanical boundary condition. Furthermore, the cells actively migrated inside the collagen matrix and promoted the expression of osteoblast and osteocyte genes with their orientations aligned along the uniaxially-fixed boundary condition. Collectively, our results suggest that the intracellular tension of osteoblasts under a uniaxially-fixed mechanical boundary condition is one of the factors that determines the osteocyte alignment inside the bone matrix.Jeonghyun KimKeiichi IshikawaJunko SunagaTaiji AdachiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jeonghyun Kim Keiichi Ishikawa Junko Sunaga Taiji Adachi Uniaxially fixed mechanical boundary condition elicits cellular alignment in collagen matrix with induction of osteogenesis
description	Abstract Osteocytes differentiated from osteoblasts play significant roles as mechanosensors in modulating the bone remodeling process. While the well-aligned osteocyte network along the trabeculae with slender cell processes perpendicular to the trabeculae surface is known to facilitate the sensing of mechanical stimuli by cells and the intracellular communication in the bone matrix, the mechanisms underlying osteocyte network formation remains unclear. Here, we developed a novel in vitro collagen matrix system exerting a uniaxially-fixed mechanical boundary condition on which mouse osteoblast-like MC3T3-E1 cells were subcultured, evoking cellular alignment along the uniaxial boundary condition. Using a myosin II inhibitor, blebbistatin, we showed that the intracellular tension via contraction of actin fibers contributed to the cellular alignment under the influence of isometric matrix condition along the uniaxially-fixed mechanical boundary condition. Furthermore, the cells actively migrated inside the collagen matrix and promoted the expression of osteoblast and osteocyte genes with their orientations aligned along the uniaxially-fixed boundary condition. Collectively, our results suggest that the intracellular tension of osteoblasts under a uniaxially-fixed mechanical boundary condition is one of the factors that determines the osteocyte alignment inside the bone matrix.
format	article
author	Jeonghyun Kim Keiichi Ishikawa Junko Sunaga Taiji Adachi
author_facet	Jeonghyun Kim Keiichi Ishikawa Junko Sunaga Taiji Adachi
author_sort	Jeonghyun Kim
title	Uniaxially fixed mechanical boundary condition elicits cellular alignment in collagen matrix with induction of osteogenesis
title_short	Uniaxially fixed mechanical boundary condition elicits cellular alignment in collagen matrix with induction of osteogenesis
title_full	Uniaxially fixed mechanical boundary condition elicits cellular alignment in collagen matrix with induction of osteogenesis
title_fullStr	Uniaxially fixed mechanical boundary condition elicits cellular alignment in collagen matrix with induction of osteogenesis
title_full_unstemmed	Uniaxially fixed mechanical boundary condition elicits cellular alignment in collagen matrix with induction of osteogenesis
title_sort	uniaxially fixed mechanical boundary condition elicits cellular alignment in collagen matrix with induction of osteogenesis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7d8c2b39cd454626b0f95c11b79de3de
work_keys_str_mv	AT jeonghyunkim uniaxiallyfixedmechanicalboundaryconditionelicitscellularalignmentincollagenmatrixwithinductionofosteogenesis AT keiichiishikawa uniaxiallyfixedmechanicalboundaryconditionelicitscellularalignmentincollagenmatrixwithinductionofosteogenesis AT junkosunaga uniaxiallyfixedmechanicalboundaryconditionelicitscellularalignmentincollagenmatrixwithinductionofosteogenesis AT taijiadachi uniaxiallyfixedmechanicalboundaryconditionelicitscellularalignmentincollagenmatrixwithinductionofosteogenesis
_version_	1718382808156602368

Uniaxially fixed mechanical boundary condition elicits cellular alignment in collagen matrix with induction of osteogenesis

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