EFFECTS OF SYNTETIC CANNABINOID RECEPTOR LIGANDS WIN 55.212-2 AND ANANDAMID UPON IN VITRO ACTIVITY OF IMMUNOCOMPETENT CELLS

Abstract. Ability of cannabinoid receptor ligands WIN 55.212-2 and anandamid to inhibit synthesis of TNFα and IL-8 was studied in healthy donors and men with allergic disorders. To establish mechanism of action for investigated substances, the selective antagonists of the СВ1-receptor (SR141716A) an...

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Autor principal:	E. G. Lobanova
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	immune cells tnfα il-8 cannabinoid receptor ligands win-55.212-2 anandamid Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/7d954e3e249a4d6d9178881ac920ca75
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spelling	oai:doaj.org-article:7d954e3e249a4d6d9178881ac920ca752021-11-18T08:03:40ZEFFECTS OF SYNTETIC CANNABINOID RECEPTOR LIGANDS WIN 55.212-2 AND ANANDAMID UPON IN VITRO ACTIVITY OF IMMUNOCOMPETENT CELLS1563-06252313-741X10.15789/1563-0625-2009-2-3-261-264https://doaj.org/article/7d954e3e249a4d6d9178881ac920ca752014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/315https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. Ability of cannabinoid receptor ligands WIN 55.212-2 and anandamid to inhibit synthesis of TNFα and IL-8 was studied in healthy donors and men with allergic disorders. To establish mechanism of action for investigated substances, the selective antagonists of the СВ1-receptor (SR141716A) and for СВ2 - receptor (SR144528) were applied. Studies with whole blood dilutions allowed of approximating in vivo conditions when investigating biological properties of WIN-55.212-2 and anandamid. The synthetic cannabinoids WIN - 55.212-2 and anandamid at a concentration of 3-10 μМ were capable of reducing synthesis of TNFα and IL-8 in lipopolysaccharide-stimulated blood leukocytes, both from healthy donors and subjects with allergic disorders. It was revealed that the antagonist of СВ1-receptor (SR141716A) did not exert a receptor-mediated effect for WIN-55.212-2 and anandamid. Meanwhile, a СВ2-receptor antagonist (SR144528) entirely eliminated completely the blocking effect of anandamid and WIN-55.212-2.E. G. LobanovaSPb RAACIarticleimmune cellstnfαil-8cannabinoid receptor ligandswin-55.212-2anandamidImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 11, Iss 2-3, Pp 261-264 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	immune cells tnfα il-8 cannabinoid receptor ligands win-55.212-2 anandamid Immunologic diseases. Allergy RC581-607
spellingShingle	immune cells tnfα il-8 cannabinoid receptor ligands win-55.212-2 anandamid Immunologic diseases. Allergy RC581-607 E. G. Lobanova EFFECTS OF SYNTETIC CANNABINOID RECEPTOR LIGANDS WIN 55.212-2 AND ANANDAMID UPON IN VITRO ACTIVITY OF IMMUNOCOMPETENT CELLS
description	Abstract. Ability of cannabinoid receptor ligands WIN 55.212-2 and anandamid to inhibit synthesis of TNFα and IL-8 was studied in healthy donors and men with allergic disorders. To establish mechanism of action for investigated substances, the selective antagonists of the СВ1-receptor (SR141716A) and for СВ2 - receptor (SR144528) were applied. Studies with whole blood dilutions allowed of approximating in vivo conditions when investigating biological properties of WIN-55.212-2 and anandamid. The synthetic cannabinoids WIN - 55.212-2 and anandamid at a concentration of 3-10 μМ were capable of reducing synthesis of TNFα and IL-8 in lipopolysaccharide-stimulated blood leukocytes, both from healthy donors and subjects with allergic disorders. It was revealed that the antagonist of СВ1-receptor (SR141716A) did not exert a receptor-mediated effect for WIN-55.212-2 and anandamid. Meanwhile, a СВ2-receptor antagonist (SR144528) entirely eliminated completely the blocking effect of anandamid and WIN-55.212-2.
format	article
author	E. G. Lobanova
author_facet	E. G. Lobanova
author_sort	E. G. Lobanova
title	EFFECTS OF SYNTETIC CANNABINOID RECEPTOR LIGANDS WIN 55.212-2 AND ANANDAMID UPON IN VITRO ACTIVITY OF IMMUNOCOMPETENT CELLS
title_short	EFFECTS OF SYNTETIC CANNABINOID RECEPTOR LIGANDS WIN 55.212-2 AND ANANDAMID UPON IN VITRO ACTIVITY OF IMMUNOCOMPETENT CELLS
title_full	EFFECTS OF SYNTETIC CANNABINOID RECEPTOR LIGANDS WIN 55.212-2 AND ANANDAMID UPON IN VITRO ACTIVITY OF IMMUNOCOMPETENT CELLS
title_fullStr	EFFECTS OF SYNTETIC CANNABINOID RECEPTOR LIGANDS WIN 55.212-2 AND ANANDAMID UPON IN VITRO ACTIVITY OF IMMUNOCOMPETENT CELLS
title_full_unstemmed	EFFECTS OF SYNTETIC CANNABINOID RECEPTOR LIGANDS WIN 55.212-2 AND ANANDAMID UPON IN VITRO ACTIVITY OF IMMUNOCOMPETENT CELLS
title_sort	effects of syntetic cannabinoid receptor ligands win 55.212-2 and anandamid upon in vitro activity of immunocompetent cells
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/7d954e3e249a4d6d9178881ac920ca75
work_keys_str_mv	AT eglobanova effectsofsynteticcannabinoidreceptorligandswin552122andanandamiduponinvitroactivityofimmunocompetentcells
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EFFECTS OF SYNTETIC CANNABINOID RECEPTOR LIGANDS WIN 55.212-2 AND ANANDAMID UPON IN VITRO ACTIVITY OF IMMUNOCOMPETENT CELLS

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