Mussel Inspired Polynorepinephrine Functionalized Electrospun Polycaprolactone Microfibers for Muscle Regeneration

Abstract Electrospun scaffolds with excellent mechanical properties, high specific surface area and a commendable porous network are widely used in tissue engineering. Improving the hydrophilicity and cell adhesion of hydrophobic substrates is the key point to enhance the effectiveness of electrospu...

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Autores principales:	Ying Liu, Guoqiang Zhou, Zhu Liu, Mengyu Guo, Xiumei Jiang, Mehmet Berat Taskin, Zhongyang Zhang, Jing Liu, Jinglong Tang, Ru Bai, Flemming Besenbacher, Menglin Chen, Chunying Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7d9787a298114aac94d9808ce7cd37c3
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spelling	oai:doaj.org-article:7d9787a298114aac94d9808ce7cd37c32021-12-02T11:40:58ZMussel Inspired Polynorepinephrine Functionalized Electrospun Polycaprolactone Microfibers for Muscle Regeneration10.1038/s41598-017-08572-z2045-2322https://doaj.org/article/7d9787a298114aac94d9808ce7cd37c32017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08572-zhttps://doaj.org/toc/2045-2322Abstract Electrospun scaffolds with excellent mechanical properties, high specific surface area and a commendable porous network are widely used in tissue engineering. Improving the hydrophilicity and cell adhesion of hydrophobic substrates is the key point to enhance the effectiveness of electrospun scaffolds. In this study, polycaprolactone (PCL) fibrous membranes with appropriate diameter were selected and coated by mussel-inspired poly norepinephrine (pNE). And norepinephrine is a catecholamine functioning as a hormone and neurotransmitter in the human brain. The membrane with smaller diameter fibers, a relative larger specific surface area and the suitable pNE functionalization provided more suitable microenvironment for cell adhesion and proliferation both in vitro and in vivo. The regenerated muscle layer can be integrated well with fibrous membranes and surrounding tissues at the impaired site and thus the mechanical strength reached the value of native tissue. The underlying molecular mechanism is mediated via inhibiting myostatin expression by PI3K/AKT/mTOR hypertrophy pathway. The properly functionalized fibrous membranes hold the potential for repairing muscle injuries. Our current work also provides an insight for rational design and development of better tissue engineering materials for skeletal muscle regeneration.Ying LiuGuoqiang ZhouZhu LiuMengyu GuoXiumei JiangMehmet Berat TaskinZhongyang ZhangJing LiuJinglong TangRu BaiFlemming BesenbacherMenglin ChenChunying ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ying Liu Guoqiang Zhou Zhu Liu Mengyu Guo Xiumei Jiang Mehmet Berat Taskin Zhongyang Zhang Jing Liu Jinglong Tang Ru Bai Flemming Besenbacher Menglin Chen Chunying Chen Mussel Inspired Polynorepinephrine Functionalized Electrospun Polycaprolactone Microfibers for Muscle Regeneration
description	Abstract Electrospun scaffolds with excellent mechanical properties, high specific surface area and a commendable porous network are widely used in tissue engineering. Improving the hydrophilicity and cell adhesion of hydrophobic substrates is the key point to enhance the effectiveness of electrospun scaffolds. In this study, polycaprolactone (PCL) fibrous membranes with appropriate diameter were selected and coated by mussel-inspired poly norepinephrine (pNE). And norepinephrine is a catecholamine functioning as a hormone and neurotransmitter in the human brain. The membrane with smaller diameter fibers, a relative larger specific surface area and the suitable pNE functionalization provided more suitable microenvironment for cell adhesion and proliferation both in vitro and in vivo. The regenerated muscle layer can be integrated well with fibrous membranes and surrounding tissues at the impaired site and thus the mechanical strength reached the value of native tissue. The underlying molecular mechanism is mediated via inhibiting myostatin expression by PI3K/AKT/mTOR hypertrophy pathway. The properly functionalized fibrous membranes hold the potential for repairing muscle injuries. Our current work also provides an insight for rational design and development of better tissue engineering materials for skeletal muscle regeneration.
format	article
author	Ying Liu Guoqiang Zhou Zhu Liu Mengyu Guo Xiumei Jiang Mehmet Berat Taskin Zhongyang Zhang Jing Liu Jinglong Tang Ru Bai Flemming Besenbacher Menglin Chen Chunying Chen
author_facet	Ying Liu Guoqiang Zhou Zhu Liu Mengyu Guo Xiumei Jiang Mehmet Berat Taskin Zhongyang Zhang Jing Liu Jinglong Tang Ru Bai Flemming Besenbacher Menglin Chen Chunying Chen
author_sort	Ying Liu
title	Mussel Inspired Polynorepinephrine Functionalized Electrospun Polycaprolactone Microfibers for Muscle Regeneration
title_short	Mussel Inspired Polynorepinephrine Functionalized Electrospun Polycaprolactone Microfibers for Muscle Regeneration
title_full	Mussel Inspired Polynorepinephrine Functionalized Electrospun Polycaprolactone Microfibers for Muscle Regeneration
title_fullStr	Mussel Inspired Polynorepinephrine Functionalized Electrospun Polycaprolactone Microfibers for Muscle Regeneration
title_full_unstemmed	Mussel Inspired Polynorepinephrine Functionalized Electrospun Polycaprolactone Microfibers for Muscle Regeneration
title_sort	mussel inspired polynorepinephrine functionalized electrospun polycaprolactone microfibers for muscle regeneration
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7d9787a298114aac94d9808ce7cd37c3
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Mussel Inspired Polynorepinephrine Functionalized Electrospun Polycaprolactone Microfibers for Muscle Regeneration

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