Identification and validation of potential novel biomarkers for oral squamous cell carcinoma

Our study aimed to explore potential new diagnostic biomarkers in patients with oral squamous cell carcinoma (OSCC) to find new target molecules involved in the progression of OSCC. Potential novel biomarkers of OSCC were identified using a protein microarray assay. Compared with the healthy control...

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Autores principales:	Mengxue Zhang, Xiao Chen, He Chen, Minyue Zhou, Yaoqiang Liu, Yali Hou, Minhai Nie, Xuqian Liu
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	oral squamous cell carcinoma biomarkers serum protein microarray assay diagnosis Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/7da029492f0d4399ad570ff30be2ad57
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spelling	oai:doaj.org-article:7da029492f0d4399ad570ff30be2ad572021-11-04T15:51:53ZIdentification and validation of potential novel biomarkers for oral squamous cell carcinoma2165-59792165-598710.1080/21655979.2021.1987089https://doaj.org/article/7da029492f0d4399ad570ff30be2ad572021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1987089https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Our study aimed to explore potential new diagnostic biomarkers in patients with oral squamous cell carcinoma (OSCC) to find new target molecules involved in the progression of OSCC. Potential novel biomarkers of OSCC were identified using a protein microarray assay. Compared with the healthy control group, there were five proteins (I309, GDF15, AXL, MMP3, and CTACK) in the serum of in situ oral cancer group. However, there were four differentially expressed proteins (MCSF, I309, MMP3, and CTACK) in the serum of the OSCC group. Receiver operating characteristic (ROC) curve analysis results suggested that these six proteins (I309, GDF15, AXL, MMP3, CTACK, and MCSF) had diagnostic value for OSCC. Based on The Cancer Genome Atlas (TCGA) database, we found that only GDF15 expression was associated with the prognosis of OSCC. Subsequently, we verified the expression levels of six proteins in HSC-3 and HaCaT cells, and the results showed that the level of these six proteins was significantly higher in HSC-3 cells than in normal HaCaT cells. Similarly, in the OSCC nude mouse model, the expression levels of these proteins were significantly upregulated in OSCC tumor tissue compared to the normal tissue. GDF15, MMP3, AXL, MCSF, I309, and CTACK may be used as biomarkers for OSCC diagnosis and provide a novel study direction for the treatment of OSCC.Mengxue ZhangXiao ChenHe ChenMinyue ZhouYaoqiang LiuYali HouMinhai NieXuqian LiuTaylor & Francis Grouparticleoral squamous cell carcinomabiomarkersserumprotein microarray assaydiagnosisBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 1, Pp 8845-8862 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	oral squamous cell carcinoma biomarkers serum protein microarray assay diagnosis Biotechnology TP248.13-248.65
spellingShingle	oral squamous cell carcinoma biomarkers serum protein microarray assay diagnosis Biotechnology TP248.13-248.65 Mengxue Zhang Xiao Chen He Chen Minyue Zhou Yaoqiang Liu Yali Hou Minhai Nie Xuqian Liu Identification and validation of potential novel biomarkers for oral squamous cell carcinoma
description	Our study aimed to explore potential new diagnostic biomarkers in patients with oral squamous cell carcinoma (OSCC) to find new target molecules involved in the progression of OSCC. Potential novel biomarkers of OSCC were identified using a protein microarray assay. Compared with the healthy control group, there were five proteins (I309, GDF15, AXL, MMP3, and CTACK) in the serum of in situ oral cancer group. However, there were four differentially expressed proteins (MCSF, I309, MMP3, and CTACK) in the serum of the OSCC group. Receiver operating characteristic (ROC) curve analysis results suggested that these six proteins (I309, GDF15, AXL, MMP3, CTACK, and MCSF) had diagnostic value for OSCC. Based on The Cancer Genome Atlas (TCGA) database, we found that only GDF15 expression was associated with the prognosis of OSCC. Subsequently, we verified the expression levels of six proteins in HSC-3 and HaCaT cells, and the results showed that the level of these six proteins was significantly higher in HSC-3 cells than in normal HaCaT cells. Similarly, in the OSCC nude mouse model, the expression levels of these proteins were significantly upregulated in OSCC tumor tissue compared to the normal tissue. GDF15, MMP3, AXL, MCSF, I309, and CTACK may be used as biomarkers for OSCC diagnosis and provide a novel study direction for the treatment of OSCC.
format	article
author	Mengxue Zhang Xiao Chen He Chen Minyue Zhou Yaoqiang Liu Yali Hou Minhai Nie Xuqian Liu
author_facet	Mengxue Zhang Xiao Chen He Chen Minyue Zhou Yaoqiang Liu Yali Hou Minhai Nie Xuqian Liu
author_sort	Mengxue Zhang
title	Identification and validation of potential novel biomarkers for oral squamous cell carcinoma
title_short	Identification and validation of potential novel biomarkers for oral squamous cell carcinoma
title_full	Identification and validation of potential novel biomarkers for oral squamous cell carcinoma
title_fullStr	Identification and validation of potential novel biomarkers for oral squamous cell carcinoma
title_full_unstemmed	Identification and validation of potential novel biomarkers for oral squamous cell carcinoma
title_sort	identification and validation of potential novel biomarkers for oral squamous cell carcinoma
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/7da029492f0d4399ad570ff30be2ad57
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Identification and validation of potential novel biomarkers for oral squamous cell carcinoma

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