RNA-Seq, physiological, and biochemical analysis of burley tobacco response to nitrogen deficiency

Abstract To explore the effects of nitrogen deficiency in burley tobacco, two varieties were cultivated and subjected to conditions of sufficient and deficient nitrogen. The natural characteristics of varieties TN90 and TN86 during tobacco cultivation were similar for nitrogen metabolism. Both carbo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yafei Li, Dong Chang, Xiang Zhang, Hongzhi Shi, Huijuan Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7da2fef62bb848c0b41686487ecfa002
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7da2fef62bb848c0b41686487ecfa002
record_format	dspace
spelling	oai:doaj.org-article:7da2fef62bb848c0b41686487ecfa0022021-12-02T15:10:50ZRNA-Seq, physiological, and biochemical analysis of burley tobacco response to nitrogen deficiency10.1038/s41598-021-93363-w2045-2322https://doaj.org/article/7da2fef62bb848c0b41686487ecfa0022021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93363-whttps://doaj.org/toc/2045-2322Abstract To explore the effects of nitrogen deficiency in burley tobacco, two varieties were cultivated and subjected to conditions of sufficient and deficient nitrogen. The natural characteristics of varieties TN90 and TN86 during tobacco cultivation were similar for nitrogen metabolism. Both carbon and nitrogen metabolism were significantly affected by reducing amounts of applied nitrogen. Under nitrogen-deficient conditions, average leaf biomass, root weight, photosynthetic rate (Pn), pigment levels, total nitrogen, and nitrate content of TN86 and TN90 were significantly decreased by 52.88%, 69.19%, 22.65%, 46.80%, 37.42%, and 79.15%, respectively (p < 0.01). Nicotine and soluble reducing sugar contents were significantly decreased by 96.67% and 95.12%, respectively, in TN86 roots (p < 0.01), which was consistent with the reductions in root surf area, average diameter, and root volume. Nitrogen deficiency induced 6318 differentially expressed genes in both TN90 and TN86, which were highly expressed. In total, 428 upregulated genes were analysed and found to be mainly enriched in the MAPK signalling pathway, sesquiterpenoid and triterpenoid biosynthesis, and arginine and proline metabolism. Meanwhile, 213 downregulated genes were analysed and found to be mainly enriched in photosynthesis, nitrogen metabolism, and amino acid biosynthesis. Reduced pigment content and Pn may result in low carbohydrate formation and decreased leaf biomass in burley tobacco under nitrogen-deficient conditions.Yafei LiDong ChangXiang ZhangHongzhi ShiHuijuan YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yafei Li Dong Chang Xiang Zhang Hongzhi Shi Huijuan Yang RNA-Seq, physiological, and biochemical analysis of burley tobacco response to nitrogen deficiency
description	Abstract To explore the effects of nitrogen deficiency in burley tobacco, two varieties were cultivated and subjected to conditions of sufficient and deficient nitrogen. The natural characteristics of varieties TN90 and TN86 during tobacco cultivation were similar for nitrogen metabolism. Both carbon and nitrogen metabolism were significantly affected by reducing amounts of applied nitrogen. Under nitrogen-deficient conditions, average leaf biomass, root weight, photosynthetic rate (Pn), pigment levels, total nitrogen, and nitrate content of TN86 and TN90 were significantly decreased by 52.88%, 69.19%, 22.65%, 46.80%, 37.42%, and 79.15%, respectively (p < 0.01). Nicotine and soluble reducing sugar contents were significantly decreased by 96.67% and 95.12%, respectively, in TN86 roots (p < 0.01), which was consistent with the reductions in root surf area, average diameter, and root volume. Nitrogen deficiency induced 6318 differentially expressed genes in both TN90 and TN86, which were highly expressed. In total, 428 upregulated genes were analysed and found to be mainly enriched in the MAPK signalling pathway, sesquiterpenoid and triterpenoid biosynthesis, and arginine and proline metabolism. Meanwhile, 213 downregulated genes were analysed and found to be mainly enriched in photosynthesis, nitrogen metabolism, and amino acid biosynthesis. Reduced pigment content and Pn may result in low carbohydrate formation and decreased leaf biomass in burley tobacco under nitrogen-deficient conditions.
format	article
author	Yafei Li Dong Chang Xiang Zhang Hongzhi Shi Huijuan Yang
author_facet	Yafei Li Dong Chang Xiang Zhang Hongzhi Shi Huijuan Yang
author_sort	Yafei Li
title	RNA-Seq, physiological, and biochemical analysis of burley tobacco response to nitrogen deficiency
title_short	RNA-Seq, physiological, and biochemical analysis of burley tobacco response to nitrogen deficiency
title_full	RNA-Seq, physiological, and biochemical analysis of burley tobacco response to nitrogen deficiency
title_fullStr	RNA-Seq, physiological, and biochemical analysis of burley tobacco response to nitrogen deficiency
title_full_unstemmed	RNA-Seq, physiological, and biochemical analysis of burley tobacco response to nitrogen deficiency
title_sort	rna-seq, physiological, and biochemical analysis of burley tobacco response to nitrogen deficiency
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7da2fef62bb848c0b41686487ecfa002
work_keys_str_mv	AT yafeili rnaseqphysiologicalandbiochemicalanalysisofburleytobaccoresponsetonitrogendeficiency AT dongchang rnaseqphysiologicalandbiochemicalanalysisofburleytobaccoresponsetonitrogendeficiency AT xiangzhang rnaseqphysiologicalandbiochemicalanalysisofburleytobaccoresponsetonitrogendeficiency AT hongzhishi rnaseqphysiologicalandbiochemicalanalysisofburleytobaccoresponsetonitrogendeficiency AT huijuanyang rnaseqphysiologicalandbiochemicalanalysisofburleytobaccoresponsetonitrogendeficiency
_version_	1718387699942948864

RNA-Seq, physiological, and biochemical analysis of burley tobacco response to nitrogen deficiency

Ejemplares similares