Association of Serum Dystroglycan, MMP-2/9 and AQP-4 with Haematoma Expansion in Patients with Intracerebral Haemorrhage

Yue Shi,1 Xuehui Fan,1 Guozhong Li,1 Di Zhong,1 Xin Zhang2 1Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, People’s Republic of China; 2Department of Neurology, Liuzhou People’s Hospital, Liuzhou, Guangxi...

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Autores principales:	Shi Y, Fan X, Li G, Zhong D, Zhang X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	intracerebral hemorrhage (ich) hematoma enlargement (he) dystroglycan(dg) matrixmetalloproteinase-2/9(mmp-2/9) aquaporin-4(aqp-4) Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
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spelling	oai:doaj.org-article:7dc0e4711671424398100f1efadb95402021-12-02T11:55:28ZAssociation of Serum Dystroglycan, MMP-2/9 and AQP-4 with Haematoma Expansion in Patients with Intracerebral Haemorrhage1178-2021https://doaj.org/article/7dc0e4711671424398100f1efadb95402021-01-01T00:00:00Zhttps://www.dovepress.com/association-of-serum-dystroglycan-mmp-29-and-aqp-4-with-haematoma-expa-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Yue Shi,1 Xuehui Fan,1 Guozhong Li,1 Di Zhong,1 Xin Zhang2 1Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, People’s Republic of China; 2Department of Neurology, Liuzhou People’s Hospital, Liuzhou, Guangxi, 545006, People’s Republic of ChinaCorrespondence: Xin ZhangDepartment of Neurology, Liuzhou People’s Hospital, 8 Wenchang Road, Liuzhou, Guangxi 545006, People’s Republic of ChinaTel +86-13946027603Email zhangxinxueshu123@163.comObjective: The purpose of this study was to explore association of serum dystroglycan (DG), matrix metalloproteinase-2/matrix metalloproteinase-9 (MMP-2/9), and aquaporin-4 (AQP-4) expression and haematoma expansion in patients with intracerebral haemorrhage (ICH), which are proteins involved in maintaining the integrity of the blood-brain barrier.Methods: We included patients older than 18 years old with ICH who had undergone baseline CT within 6 hours after intracerebral haemorrhage symptom onset in our hospital between April 2018 and December 2018. Two readers independently assessed haematoma volume and other imaging information upon admission and again within 24 hours. All patients underwent 5 mL of venous blood collection 6 and 24 hours after admission. Serum expression levels of dystroglycan, matrix metalloproteinase-2/matrix metalloproteinase-9 and aquaporin-4 were determined by quantitative enzyme-linked immunosorbent assay (ELISA). Repeated analysis of variance was used to determine whether expression of the four proteins in patients with cerebral haemorrhage changed within 24 hours and whether there were differences between the haematoma enlargement and non-haematoma enlargement groups over time. Univariate and multivariate logistic regression analyses were used to compare the correlation among expression of the four proteins, clinical characteristics of patients and haematoma enlargement.Results: Expression levels of serum matrix metalloproteinase-2/matrix metalloproteinases-9 and aquaporin-4 gradually increased within 24 hours in patients with cerebral haemorrhage (P< 0.001), while expression levels of dystroglycan gradually decreased (P< 0.01). Expression of serum matrix metalloproteinases-9 6 hours after onset was independently correlated with the expansion of cerebral haemorrhage. The ROC curve (AUC=0.778, 95% Cl: 0.661– 0.894, P< 0.001) exhibited high sensitivity (0.900) and low specificity (0.642).Conclusion: These data support that expression of MMP-9 in peripheral blood is independently correlated with the enlargement of haematoma in patients with intracerebral haemorrhage 6 hours after onset and can be used as an independent predictor of haematoma enlargement in patients with intracerebral haemorrhage. However, although the expression of MMP-2, AQP-4 and DG exhibited some changes within 6 and 24 hours after onset, they were not independently correlated with early haematoma enlargement in patients with intracerebral haemorrhage. Further multi-time point exploration and expansion of the sample size is necessary in future studies.Keywords: intracerebral hemorrhage, ICH, hematoma enlargement, HE, dystroglycan, DG, matrix metalloproteinase-2/9, MMP-2/9, aquaporin-4, AQP-4Shi YFan XLi GZhong DZhang XDove Medical Pressarticleintracerebral hemorrhage (ich)hematoma enlargement (he)dystroglycan(dg)matrixmetalloproteinase-2/9(mmp-2/9)aquaporin-4(aqp-4)Neurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 17, Pp 11-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	intracerebral hemorrhage (ich) hematoma enlargement (he) dystroglycan(dg) matrixmetalloproteinase-2/9(mmp-2/9) aquaporin-4(aqp-4) Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	intracerebral hemorrhage (ich) hematoma enlargement (he) dystroglycan(dg) matrixmetalloproteinase-2/9(mmp-2/9) aquaporin-4(aqp-4) Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Shi Y Fan X Li G Zhong D Zhang X Association of Serum Dystroglycan, MMP-2/9 and AQP-4 with Haematoma Expansion in Patients with Intracerebral Haemorrhage
description	Yue Shi,1 Xuehui Fan,1 Guozhong Li,1 Di Zhong,1 Xin Zhang2 1Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, People’s Republic of China; 2Department of Neurology, Liuzhou People’s Hospital, Liuzhou, Guangxi, 545006, People’s Republic of ChinaCorrespondence: Xin ZhangDepartment of Neurology, Liuzhou People’s Hospital, 8 Wenchang Road, Liuzhou, Guangxi 545006, People’s Republic of ChinaTel +86-13946027603Email zhangxinxueshu123@163.comObjective: The purpose of this study was to explore association of serum dystroglycan (DG), matrix metalloproteinase-2/matrix metalloproteinase-9 (MMP-2/9), and aquaporin-4 (AQP-4) expression and haematoma expansion in patients with intracerebral haemorrhage (ICH), which are proteins involved in maintaining the integrity of the blood-brain barrier.Methods: We included patients older than 18 years old with ICH who had undergone baseline CT within 6 hours after intracerebral haemorrhage symptom onset in our hospital between April 2018 and December 2018. Two readers independently assessed haematoma volume and other imaging information upon admission and again within 24 hours. All patients underwent 5 mL of venous blood collection 6 and 24 hours after admission. Serum expression levels of dystroglycan, matrix metalloproteinase-2/matrix metalloproteinase-9 and aquaporin-4 were determined by quantitative enzyme-linked immunosorbent assay (ELISA). Repeated analysis of variance was used to determine whether expression of the four proteins in patients with cerebral haemorrhage changed within 24 hours and whether there were differences between the haematoma enlargement and non-haematoma enlargement groups over time. Univariate and multivariate logistic regression analyses were used to compare the correlation among expression of the four proteins, clinical characteristics of patients and haematoma enlargement.Results: Expression levels of serum matrix metalloproteinase-2/matrix metalloproteinases-9 and aquaporin-4 gradually increased within 24 hours in patients with cerebral haemorrhage (P< 0.001), while expression levels of dystroglycan gradually decreased (P< 0.01). Expression of serum matrix metalloproteinases-9 6 hours after onset was independently correlated with the expansion of cerebral haemorrhage. The ROC curve (AUC=0.778, 95% Cl: 0.661– 0.894, P< 0.001) exhibited high sensitivity (0.900) and low specificity (0.642).Conclusion: These data support that expression of MMP-9 in peripheral blood is independently correlated with the enlargement of haematoma in patients with intracerebral haemorrhage 6 hours after onset and can be used as an independent predictor of haematoma enlargement in patients with intracerebral haemorrhage. However, although the expression of MMP-2, AQP-4 and DG exhibited some changes within 6 and 24 hours after onset, they were not independently correlated with early haematoma enlargement in patients with intracerebral haemorrhage. Further multi-time point exploration and expansion of the sample size is necessary in future studies.Keywords: intracerebral hemorrhage, ICH, hematoma enlargement, HE, dystroglycan, DG, matrix metalloproteinase-2/9, MMP-2/9, aquaporin-4, AQP-4
format	article
author	Shi Y Fan X Li G Zhong D Zhang X
author_facet	Shi Y Fan X Li G Zhong D Zhang X
author_sort	Shi Y
title	Association of Serum Dystroglycan, MMP-2/9 and AQP-4 with Haematoma Expansion in Patients with Intracerebral Haemorrhage
title_short	Association of Serum Dystroglycan, MMP-2/9 and AQP-4 with Haematoma Expansion in Patients with Intracerebral Haemorrhage
title_full	Association of Serum Dystroglycan, MMP-2/9 and AQP-4 with Haematoma Expansion in Patients with Intracerebral Haemorrhage
title_fullStr	Association of Serum Dystroglycan, MMP-2/9 and AQP-4 with Haematoma Expansion in Patients with Intracerebral Haemorrhage
title_full_unstemmed	Association of Serum Dystroglycan, MMP-2/9 and AQP-4 with Haematoma Expansion in Patients with Intracerebral Haemorrhage
title_sort	association of serum dystroglycan, mmp-2/9 and aqp-4 with haematoma expansion in patients with intracerebral haemorrhage
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/7dc0e4711671424398100f1efadb9540
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Association of Serum Dystroglycan, MMP-2/9 and AQP-4 with Haematoma Expansion in Patients with Intracerebral Haemorrhage

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