VIRAL ETIOLOGY ACUTE INTESTINAL INFECTIONS MOLECULAR MONITORING IN CHILDREN’S HOSPITAL

On the territory of the Russian Federation in the overall structure of acute intestinal infections the proportion of viral diarrhea among children varies from 24 to 78% of cases depending on the season. The acute viral intestinal infections etiological confirmation is performed mainly among patients...

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Autores principales:	A. V. Sergeeva, L. Y. Poslova, O. V. Kovalishena, A. S. Blagonravova, N. V. Epifanova, T. A. Sashina, O. V. Morozova, N. A. Novikova
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2015
Materias:	noroviruses rotaviruses adenoviruses astroviruses acute intestinal infection molecular-genetic monitoring Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/7dc3e65bf85343fdaeb78fb65f48a877
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spelling	oai:doaj.org-article:7dc3e65bf85343fdaeb78fb65f48a8772021-11-22T07:09:47ZVIRAL ETIOLOGY ACUTE INTESTINAL INFECTIONS MOLECULAR MONITORING IN CHILDREN’S HOSPITAL2220-76192313-739810.15789/2220-7619-2015-3-243-252https://doaj.org/article/7dc3e65bf85343fdaeb78fb65f48a8772015-12-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/329https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398On the territory of the Russian Federation in the overall structure of acute intestinal infections the proportion of viral diarrhea among children varies from 24 to 78% of cases depending on the season. The acute viral intestinal infections etiological confirmation is performed mainly among patients of infectious hospitals. The prevalence of viral acute intestinal infections in non-infectious hospitals, including infections associated with medical care, remains unclear. Currently estimation of viral component in the acute intestinal infections overall structure mainly consists in determination of rotavirus infection prevalence excluding other pathogens. As the part of viral etiology hospital infections epidemiological surveillance in non-infections children’s hospital the study of acute viral intestinal infections etiological structure and molecular genetics characterization of identified enteric viruses is conducted. The syndrome diagnosis of acute intestinal infections cases was introduced — an identification and evaluation of patients with signs of dysfunction of the gastrointestinal tract, that is not related to the underlying disease. A set of laboratory methods included identification of various intestinal pathogens DNA (RNA) by PCR-RT method; genotyping of enteric viruses using sequencing; nucleotide sequence analysis of cDNA fragments using the BLAST software package for identification of closely related strains and an online service for automatic genotyping of noroviruses by Norovirus Genotyping Tool Version 1.0. Alignment of nucleotide sequences and phylogenetic analysis was performed using the software MEGA 5.0. The obtained sequence fragments of the genome was downloaded in GenBank international database. The use of molecular genetics research methods allowed to differentiate viral pathogens of acute intestinal infections and to establish the fact of nosocomial transmission. The proportion of viral etiology acute intestinal infections in patients with clinical signs of intestinal infection, and contact persons was 43.8%. The etiological structure of intestinal virus infections was presented by noroviruses (73.2%) genotypes GII.1, GII.3, GII.4 Sydney 2012, rotaviruses (23.2%) genotypes G4P[8] and G1-IP[8], and adenovirus (1.8%) of the group F and astroviruses (1.8%) genotypes 1 and 2. Among the hospitalized children were 9 cases of viral etiology acute intestinal infections importation, and 66 of cases were of nosocomial origin. Examination of the environment revealed the presence of contamination by enteric viruses in 47.8% of cases.A. V. SergeevaL. Y. PoslovaO. V. KovalishenaA. S. BlagonravovaN. V. EpifanovaT. A. SashinaO. V. MorozovaN. A. NovikovaSankt-Peterburg : NIIÈM imeni Pasteraarticlenorovirusesrotavirusesadenovirusesastrovirusesacute intestinal infectionmolecular-genetic monitoringInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 5, Iss 3, Pp 243-252 (2015)
institution	DOAJ
collection	DOAJ
language	RU
topic	noroviruses rotaviruses adenoviruses astroviruses acute intestinal infection molecular-genetic monitoring Infectious and parasitic diseases RC109-216
spellingShingle	noroviruses rotaviruses adenoviruses astroviruses acute intestinal infection molecular-genetic monitoring Infectious and parasitic diseases RC109-216 A. V. Sergeeva L. Y. Poslova O. V. Kovalishena A. S. Blagonravova N. V. Epifanova T. A. Sashina O. V. Morozova N. A. Novikova VIRAL ETIOLOGY ACUTE INTESTINAL INFECTIONS MOLECULAR MONITORING IN CHILDREN’S HOSPITAL
description	On the territory of the Russian Federation in the overall structure of acute intestinal infections the proportion of viral diarrhea among children varies from 24 to 78% of cases depending on the season. The acute viral intestinal infections etiological confirmation is performed mainly among patients of infectious hospitals. The prevalence of viral acute intestinal infections in non-infectious hospitals, including infections associated with medical care, remains unclear. Currently estimation of viral component in the acute intestinal infections overall structure mainly consists in determination of rotavirus infection prevalence excluding other pathogens. As the part of viral etiology hospital infections epidemiological surveillance in non-infections children’s hospital the study of acute viral intestinal infections etiological structure and molecular genetics characterization of identified enteric viruses is conducted. The syndrome diagnosis of acute intestinal infections cases was introduced — an identification and evaluation of patients with signs of dysfunction of the gastrointestinal tract, that is not related to the underlying disease. A set of laboratory methods included identification of various intestinal pathogens DNA (RNA) by PCR-RT method; genotyping of enteric viruses using sequencing; nucleotide sequence analysis of cDNA fragments using the BLAST software package for identification of closely related strains and an online service for automatic genotyping of noroviruses by Norovirus Genotyping Tool Version 1.0. Alignment of nucleotide sequences and phylogenetic analysis was performed using the software MEGA 5.0. The obtained sequence fragments of the genome was downloaded in GenBank international database. The use of molecular genetics research methods allowed to differentiate viral pathogens of acute intestinal infections and to establish the fact of nosocomial transmission. The proportion of viral etiology acute intestinal infections in patients with clinical signs of intestinal infection, and contact persons was 43.8%. The etiological structure of intestinal virus infections was presented by noroviruses (73.2%) genotypes GII.1, GII.3, GII.4 Sydney 2012, rotaviruses (23.2%) genotypes G4P[8] and G1-IP[8], and adenovirus (1.8%) of the group F and astroviruses (1.8%) genotypes 1 and 2. Among the hospitalized children were 9 cases of viral etiology acute intestinal infections importation, and 66 of cases were of nosocomial origin. Examination of the environment revealed the presence of contamination by enteric viruses in 47.8% of cases.
format	article
author	A. V. Sergeeva L. Y. Poslova O. V. Kovalishena A. S. Blagonravova N. V. Epifanova T. A. Sashina O. V. Morozova N. A. Novikova
author_facet	A. V. Sergeeva L. Y. Poslova O. V. Kovalishena A. S. Blagonravova N. V. Epifanova T. A. Sashina O. V. Morozova N. A. Novikova
author_sort	A. V. Sergeeva
title	VIRAL ETIOLOGY ACUTE INTESTINAL INFECTIONS MOLECULAR MONITORING IN CHILDREN’S HOSPITAL
title_short	VIRAL ETIOLOGY ACUTE INTESTINAL INFECTIONS MOLECULAR MONITORING IN CHILDREN’S HOSPITAL
title_full	VIRAL ETIOLOGY ACUTE INTESTINAL INFECTIONS MOLECULAR MONITORING IN CHILDREN’S HOSPITAL
title_fullStr	VIRAL ETIOLOGY ACUTE INTESTINAL INFECTIONS MOLECULAR MONITORING IN CHILDREN’S HOSPITAL
title_full_unstemmed	VIRAL ETIOLOGY ACUTE INTESTINAL INFECTIONS MOLECULAR MONITORING IN CHILDREN’S HOSPITAL
title_sort	viral etiology acute intestinal infections molecular monitoring in children’s hospital
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2015
url	https://doaj.org/article/7dc3e65bf85343fdaeb78fb65f48a877
work_keys_str_mv	AT avsergeeva viraletiologyacuteintestinalinfectionsmolecularmonitoringinchildrenshospital AT lyposlova viraletiologyacuteintestinalinfectionsmolecularmonitoringinchildrenshospital AT ovkovalishena viraletiologyacuteintestinalinfectionsmolecularmonitoringinchildrenshospital AT asblagonravova viraletiologyacuteintestinalinfectionsmolecularmonitoringinchildrenshospital AT nvepifanova viraletiologyacuteintestinalinfectionsmolecularmonitoringinchildrenshospital AT tasashina viraletiologyacuteintestinalinfectionsmolecularmonitoringinchildrenshospital AT ovmorozova viraletiologyacuteintestinalinfectionsmolecularmonitoringinchildrenshospital AT nanovikova viraletiologyacuteintestinalinfectionsmolecularmonitoringinchildrenshospital
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VIRAL ETIOLOGY ACUTE INTESTINAL INFECTIONS MOLECULAR MONITORING IN CHILDREN’S HOSPITAL

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