Characterizing Microbiomes via Sequencing of Marker Loci: Techniques To Improve Throughput, Account for Cross-Contamination, and Reduce Cost

New methods to characterize microbiomes reduce technology-imposed limitations to study design, but many new approaches have not been widely adopted. Here, we present techniques to increase throughput and reduce contamination alongside a thorough review of current best practices.

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Detalles Bibliográficos
Autores principales:	Joshua G. Harrison, Gregory D. Randolph, C. Alex Buerkle
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2021
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/7dc421dccbc44ca3a230a504b487a3bf
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spelling	oai:doaj.org-article:7dc421dccbc44ca3a230a504b487a3bf2021-12-02T19:42:55ZCharacterizing Microbiomes via Sequencing of Marker Loci: Techniques To Improve Throughput, Account for Cross-Contamination, and Reduce Cost2379-507710.1128/mSystems.00294-21https://doaj.org/article/7dc421dccbc44ca3a230a504b487a3bf2021-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00294-21https://doaj.org/toc/2379-5077 New methods to characterize microbiomes reduce technology-imposed limitations to study design, but many new approaches have not been widely adopted. Here, we present techniques to increase throughput and reduce contamination alongside a thorough review of current best practices.Joshua G. HarrisonGregory D. RandolphC. Alex BuerkleAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmSystems, Vol 6, Iss 4 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Joshua G. Harrison Gregory D. Randolph C. Alex Buerkle Characterizing Microbiomes via Sequencing of Marker Loci: Techniques To Improve Throughput, Account for Cross-Contamination, and Reduce Cost
description	New methods to characterize microbiomes reduce technology-imposed limitations to study design, but many new approaches have not been widely adopted. Here, we present techniques to increase throughput and reduce contamination alongside a thorough review of current best practices.
format	article
author	Joshua G. Harrison Gregory D. Randolph C. Alex Buerkle
author_facet	Joshua G. Harrison Gregory D. Randolph C. Alex Buerkle
author_sort	Joshua G. Harrison
title	Characterizing Microbiomes via Sequencing of Marker Loci: Techniques To Improve Throughput, Account for Cross-Contamination, and Reduce Cost
title_short	Characterizing Microbiomes via Sequencing of Marker Loci: Techniques To Improve Throughput, Account for Cross-Contamination, and Reduce Cost
title_full	Characterizing Microbiomes via Sequencing of Marker Loci: Techniques To Improve Throughput, Account for Cross-Contamination, and Reduce Cost
title_fullStr	Characterizing Microbiomes via Sequencing of Marker Loci: Techniques To Improve Throughput, Account for Cross-Contamination, and Reduce Cost
title_full_unstemmed	Characterizing Microbiomes via Sequencing of Marker Loci: Techniques To Improve Throughput, Account for Cross-Contamination, and Reduce Cost
title_sort	characterizing microbiomes via sequencing of marker loci: techniques to improve throughput, account for cross-contamination, and reduce cost
publisher	American Society for Microbiology
publishDate	2021
url	https://doaj.org/article/7dc421dccbc44ca3a230a504b487a3bf
work_keys_str_mv	AT joshuagharrison characterizingmicrobiomesviasequencingofmarkerlocitechniquestoimprovethroughputaccountforcrosscontaminationandreducecost AT gregorydrandolph characterizingmicrobiomesviasequencingofmarkerlocitechniquestoimprovethroughputaccountforcrosscontaminationandreducecost AT calexbuerkle characterizingmicrobiomesviasequencingofmarkerlocitechniquestoimprovethroughputaccountforcrosscontaminationandreducecost
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Characterizing Microbiomes via Sequencing of Marker Loci: Techniques To Improve Throughput, Account for Cross-Contamination, and Reduce Cost

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