Light Scattering By Optically-Trapped Vesicles Affords Unprecedented Temporal Resolution Of Lipid-Raft Dynamics

Abstract A spectroscopic technique is presented that is able to identify rapid changes in the bending modulus and fluidity of vesicle lipid bilayers on the micrometer scale, and distinguish between the presence and absence of heterogeneities in lipid-packing order. Individual unilamellar vesicles ha...

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Autores principales:	Liam Collard, David Perez-Guaita, Bayan H. A. Faraj, Bayden R. Wood, Russell Wallis, Peter W. Andrew, Andrew J. Hudson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7dc54b85b5f0487bad112e1f39b138b8
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spelling	oai:doaj.org-article:7dc54b85b5f0487bad112e1f39b138b82021-12-02T11:40:23ZLight Scattering By Optically-Trapped Vesicles Affords Unprecedented Temporal Resolution Of Lipid-Raft Dynamics10.1038/s41598-017-08980-12045-2322https://doaj.org/article/7dc54b85b5f0487bad112e1f39b138b82017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08980-1https://doaj.org/toc/2045-2322Abstract A spectroscopic technique is presented that is able to identify rapid changes in the bending modulus and fluidity of vesicle lipid bilayers on the micrometer scale, and distinguish between the presence and absence of heterogeneities in lipid-packing order. Individual unilamellar vesicles have been isolated using laser tweezers and, by measuring the intensity modulation of elastic back-scattered light, changes in the biophysical properties of lipid bilayers were revealed. Our approach offers unprecedented temporal resolution and, uniquely, physical transformations of lipid bilayers can be monitored on a length scale of micrometers. As an example, the deformation of a membrane bilayer following the gel-to-fluid phase transition in a pure phospholipid vesicle was observed to take place across an interval of 54 ± 5 ms corresponding to an estimated full-width of only ~1 m°C. Dynamic heterogeneities in packing order were detected in mixed-lipid bilayers. Using a ternary mixture of lipids, the modulated-intensity profile of elastic back-scattered light from an optically-trapped vesicle revealed an abrupt change in the bending modulus of the bilayer which could be associated with the dissolution of ordered microdomains (i.e., lipid rafts). This occurred across an interval of 30 ± 5 ms (equivalent to ~1 m°C).Liam CollardDavid Perez-GuaitaBayan H. A. FarajBayden R. WoodRussell WallisPeter W. AndrewAndrew J. HudsonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Liam Collard David Perez-Guaita Bayan H. A. Faraj Bayden R. Wood Russell Wallis Peter W. Andrew Andrew J. Hudson Light Scattering By Optically-Trapped Vesicles Affords Unprecedented Temporal Resolution Of Lipid-Raft Dynamics
description	Abstract A spectroscopic technique is presented that is able to identify rapid changes in the bending modulus and fluidity of vesicle lipid bilayers on the micrometer scale, and distinguish between the presence and absence of heterogeneities in lipid-packing order. Individual unilamellar vesicles have been isolated using laser tweezers and, by measuring the intensity modulation of elastic back-scattered light, changes in the biophysical properties of lipid bilayers were revealed. Our approach offers unprecedented temporal resolution and, uniquely, physical transformations of lipid bilayers can be monitored on a length scale of micrometers. As an example, the deformation of a membrane bilayer following the gel-to-fluid phase transition in a pure phospholipid vesicle was observed to take place across an interval of 54 ± 5 ms corresponding to an estimated full-width of only ~1 m°C. Dynamic heterogeneities in packing order were detected in mixed-lipid bilayers. Using a ternary mixture of lipids, the modulated-intensity profile of elastic back-scattered light from an optically-trapped vesicle revealed an abrupt change in the bending modulus of the bilayer which could be associated with the dissolution of ordered microdomains (i.e., lipid rafts). This occurred across an interval of 30 ± 5 ms (equivalent to ~1 m°C).
format	article
author	Liam Collard David Perez-Guaita Bayan H. A. Faraj Bayden R. Wood Russell Wallis Peter W. Andrew Andrew J. Hudson
author_facet	Liam Collard David Perez-Guaita Bayan H. A. Faraj Bayden R. Wood Russell Wallis Peter W. Andrew Andrew J. Hudson
author_sort	Liam Collard
title	Light Scattering By Optically-Trapped Vesicles Affords Unprecedented Temporal Resolution Of Lipid-Raft Dynamics
title_short	Light Scattering By Optically-Trapped Vesicles Affords Unprecedented Temporal Resolution Of Lipid-Raft Dynamics
title_full	Light Scattering By Optically-Trapped Vesicles Affords Unprecedented Temporal Resolution Of Lipid-Raft Dynamics
title_fullStr	Light Scattering By Optically-Trapped Vesicles Affords Unprecedented Temporal Resolution Of Lipid-Raft Dynamics
title_full_unstemmed	Light Scattering By Optically-Trapped Vesicles Affords Unprecedented Temporal Resolution Of Lipid-Raft Dynamics
title_sort	light scattering by optically-trapped vesicles affords unprecedented temporal resolution of lipid-raft dynamics
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7dc54b85b5f0487bad112e1f39b138b8
work_keys_str_mv	AT liamcollard lightscatteringbyopticallytrappedvesiclesaffordsunprecedentedtemporalresolutionoflipidraftdynamics AT davidperezguaita lightscatteringbyopticallytrappedvesiclesaffordsunprecedentedtemporalresolutionoflipidraftdynamics AT bayanhafaraj lightscatteringbyopticallytrappedvesiclesaffordsunprecedentedtemporalresolutionoflipidraftdynamics AT baydenrwood lightscatteringbyopticallytrappedvesiclesaffordsunprecedentedtemporalresolutionoflipidraftdynamics AT russellwallis lightscatteringbyopticallytrappedvesiclesaffordsunprecedentedtemporalresolutionoflipidraftdynamics AT peterwandrew lightscatteringbyopticallytrappedvesiclesaffordsunprecedentedtemporalresolutionoflipidraftdynamics AT andrewjhudson lightscatteringbyopticallytrappedvesiclesaffordsunprecedentedtemporalresolutionoflipidraftdynamics
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Light Scattering By Optically-Trapped Vesicles Affords Unprecedented Temporal Resolution Of Lipid-Raft Dynamics

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