Protocol for recording the discharge of locus coeruleus neurons in free-moving mice during different sleep-wake stages
Summary: Cortical electroencephalography (EEG) is generally used to detect the different sleep-wake states of animals. EEG combined with in vivo multichannel recording provides a powerful tool for decoding the neural network of sleep-wake regulation. Here, we detail a protocol using cortical EEG com...
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| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/7dd039aad9864066835815471cd4309b |
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| Sumario: | Summary: Cortical electroencephalography (EEG) is generally used to detect the different sleep-wake states of animals. EEG combined with in vivo multichannel recording provides a powerful tool for decoding the neural network of sleep-wake regulation. Here, we detail a protocol using cortical EEG combined with in vivo multichannel recording to examine the activity of locus coeruleus (LC) neurons in free-moving mice at different sleep-wake stages. The procedures for electrode fabrication, the surgery to implant electrodes, and post-recording data analysis are also included.For complete details on the use and execution of this protocol, please refer to Liang et al. (2021). |
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