Protocol for recording the discharge of locus coeruleus neurons in free-moving mice during different sleep-wake stages

Summary: Cortical electroencephalography (EEG) is generally used to detect the different sleep-wake states of animals. EEG combined with in vivo multichannel recording provides a powerful tool for decoding the neural network of sleep-wake regulation. Here, we detail a protocol using cortical EEG com...

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Autores principales:	Yue Liang, Wu Shi, Dandan Hu, Anfeng Xiang, Ling Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cell Biology Model Organisms Neuroscience Behavior Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/7dd039aad9864066835815471cd4309b
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spelling	oai:doaj.org-article:7dd039aad9864066835815471cd4309b2021-12-04T04:35:59ZProtocol for recording the discharge of locus coeruleus neurons in free-moving mice during different sleep-wake stages2666-166710.1016/j.xpro.2021.100981https://doaj.org/article/7dd039aad9864066835815471cd4309b2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006870https://doaj.org/toc/2666-1667Summary: Cortical electroencephalography (EEG) is generally used to detect the different sleep-wake states of animals. EEG combined with in vivo multichannel recording provides a powerful tool for decoding the neural network of sleep-wake regulation. Here, we detail a protocol using cortical EEG combined with in vivo multichannel recording to examine the activity of locus coeruleus (LC) neurons in free-moving mice at different sleep-wake stages. The procedures for electrode fabrication, the surgery to implant electrodes, and post-recording data analysis are also included.For complete details on the use and execution of this protocol, please refer to Liang et al. (2021).Yue LiangWu ShiDandan HuAnfeng XiangLing ZhangElsevierarticleCell BiologyModel OrganismsNeuroscienceBehaviorScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100981- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell Biology Model Organisms Neuroscience Behavior Science (General) Q1-390
spellingShingle	Cell Biology Model Organisms Neuroscience Behavior Science (General) Q1-390 Yue Liang Wu Shi Dandan Hu Anfeng Xiang Ling Zhang Protocol for recording the discharge of locus coeruleus neurons in free-moving mice during different sleep-wake stages
description	Summary: Cortical electroencephalography (EEG) is generally used to detect the different sleep-wake states of animals. EEG combined with in vivo multichannel recording provides a powerful tool for decoding the neural network of sleep-wake regulation. Here, we detail a protocol using cortical EEG combined with in vivo multichannel recording to examine the activity of locus coeruleus (LC) neurons in free-moving mice at different sleep-wake stages. The procedures for electrode fabrication, the surgery to implant electrodes, and post-recording data analysis are also included.For complete details on the use and execution of this protocol, please refer to Liang et al. (2021).
format	article
author	Yue Liang Wu Shi Dandan Hu Anfeng Xiang Ling Zhang
author_facet	Yue Liang Wu Shi Dandan Hu Anfeng Xiang Ling Zhang
author_sort	Yue Liang
title	Protocol for recording the discharge of locus coeruleus neurons in free-moving mice during different sleep-wake stages
title_short	Protocol for recording the discharge of locus coeruleus neurons in free-moving mice during different sleep-wake stages
title_full	Protocol for recording the discharge of locus coeruleus neurons in free-moving mice during different sleep-wake stages
title_fullStr	Protocol for recording the discharge of locus coeruleus neurons in free-moving mice during different sleep-wake stages
title_full_unstemmed	Protocol for recording the discharge of locus coeruleus neurons in free-moving mice during different sleep-wake stages
title_sort	protocol for recording the discharge of locus coeruleus neurons in free-moving mice during different sleep-wake stages
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/7dd039aad9864066835815471cd4309b
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Protocol for recording the discharge of locus coeruleus neurons in free-moving mice during different sleep-wake stages

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