Functional analysis of BPSS2242 reveals its detoxification role in Burkholderia pseudomallei under salt stress

Abstract A bpss2242 gene, encoding a putative short-chain dehydrogenase/oxidoreductase (SDR) in Burkholderia pseudomallei, was identified and its expression was up-regulated by ten-fold when B. pseudomallei was cultured under high salt concentration. Previous study suggested that BPSS2242 plays impo...

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Autores principales:	Kamonwan Chamchoy, Pornpan Pumirat, Onrapak Reamtong, Danaya Pakotiprapha, Ubolsree Leartsakulpanich, Usa Boonyuen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7dd13f78dc164d7e969fb6b831414552
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spelling	oai:doaj.org-article:7dd13f78dc164d7e969fb6b8314145522021-12-02T17:44:55ZFunctional analysis of BPSS2242 reveals its detoxification role in Burkholderia pseudomallei under salt stress10.1038/s41598-020-67382-y2045-2322https://doaj.org/article/7dd13f78dc164d7e969fb6b8314145522020-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-67382-yhttps://doaj.org/toc/2045-2322Abstract A bpss2242 gene, encoding a putative short-chain dehydrogenase/oxidoreductase (SDR) in Burkholderia pseudomallei, was identified and its expression was up-regulated by ten-fold when B. pseudomallei was cultured under high salt concentration. Previous study suggested that BPSS2242 plays important roles in adaptation to salt stress and pathogenesis; however, its biological functions are still unknown. Herein, we report the biochemical properties and functional characterization of BPSS2242 from B. pseudomallei. BPSS2242 exhibited NADPH-dependent reductase activity toward diacetyl and methylglyoxal, toxic electrophilic dicarbonyls. The conserved catalytic triad was identified and found to play critical roles in catalysis and cofactor binding. Tyr162 and Lys166 are involved in NADPH binding and mutation of Lys166 causes a conformational change, altering protein structure. Overexpression of BPSS2242 in Escherichia coli increased bacterial survival upon exposure to diacetyl and methylglyoxal. Importantly, the viability of B. pseudomallei encountered dicarbonyl toxicity was enhanced when cultured under high salt concentration as a result of BPSS2242 overexpression. This is the first study demonstrating that BPSS2242 is responsible for detoxification of toxic metabolites, constituting a protective system against reactive carbonyl compounds in B. pseudomallei..Kamonwan ChamchoyPornpan PumiratOnrapak ReamtongDanaya PakotipraphaUbolsree LeartsakulpanichUsa BoonyuenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kamonwan Chamchoy Pornpan Pumirat Onrapak Reamtong Danaya Pakotiprapha Ubolsree Leartsakulpanich Usa Boonyuen Functional analysis of BPSS2242 reveals its detoxification role in Burkholderia pseudomallei under salt stress
description	Abstract A bpss2242 gene, encoding a putative short-chain dehydrogenase/oxidoreductase (SDR) in Burkholderia pseudomallei, was identified and its expression was up-regulated by ten-fold when B. pseudomallei was cultured under high salt concentration. Previous study suggested that BPSS2242 plays important roles in adaptation to salt stress and pathogenesis; however, its biological functions are still unknown. Herein, we report the biochemical properties and functional characterization of BPSS2242 from B. pseudomallei. BPSS2242 exhibited NADPH-dependent reductase activity toward diacetyl and methylglyoxal, toxic electrophilic dicarbonyls. The conserved catalytic triad was identified and found to play critical roles in catalysis and cofactor binding. Tyr162 and Lys166 are involved in NADPH binding and mutation of Lys166 causes a conformational change, altering protein structure. Overexpression of BPSS2242 in Escherichia coli increased bacterial survival upon exposure to diacetyl and methylglyoxal. Importantly, the viability of B. pseudomallei encountered dicarbonyl toxicity was enhanced when cultured under high salt concentration as a result of BPSS2242 overexpression. This is the first study demonstrating that BPSS2242 is responsible for detoxification of toxic metabolites, constituting a protective system against reactive carbonyl compounds in B. pseudomallei..
format	article
author	Kamonwan Chamchoy Pornpan Pumirat Onrapak Reamtong Danaya Pakotiprapha Ubolsree Leartsakulpanich Usa Boonyuen
author_facet	Kamonwan Chamchoy Pornpan Pumirat Onrapak Reamtong Danaya Pakotiprapha Ubolsree Leartsakulpanich Usa Boonyuen
author_sort	Kamonwan Chamchoy
title	Functional analysis of BPSS2242 reveals its detoxification role in Burkholderia pseudomallei under salt stress
title_short	Functional analysis of BPSS2242 reveals its detoxification role in Burkholderia pseudomallei under salt stress
title_full	Functional analysis of BPSS2242 reveals its detoxification role in Burkholderia pseudomallei under salt stress
title_fullStr	Functional analysis of BPSS2242 reveals its detoxification role in Burkholderia pseudomallei under salt stress
title_full_unstemmed	Functional analysis of BPSS2242 reveals its detoxification role in Burkholderia pseudomallei under salt stress
title_sort	functional analysis of bpss2242 reveals its detoxification role in burkholderia pseudomallei under salt stress
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/7dd13f78dc164d7e969fb6b831414552
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Functional analysis of BPSS2242 reveals its detoxification role in Burkholderia pseudomallei under salt stress

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