Viruses associated with ovarian degeneration in Apis mellifera L. queens.

Queen fecundity is a critical issue for the health of honeybee (Apis mellifera L.) colonies, as she is the only reproductive female in the colony and responsible for the constant renewal of the worker bee population. Any factor affecting the queen's fecundity will stagnate colony development, i...

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Autores principales:	Laurent Gauthier, Marc Ravallec, Magali Tournaire, François Cousserans, Max Bergoin, Benjamin Dainat, Joachim R de Miranda
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7dd4ed6023654021be9db5a89bcdd5e9
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spelling	oai:doaj.org-article:7dd4ed6023654021be9db5a89bcdd5e92021-11-18T06:59:57ZViruses associated with ovarian degeneration in Apis mellifera L. queens.1932-620310.1371/journal.pone.0016217https://doaj.org/article/7dd4ed6023654021be9db5a89bcdd5e92011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21283547/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Queen fecundity is a critical issue for the health of honeybee (Apis mellifera L.) colonies, as she is the only reproductive female in the colony and responsible for the constant renewal of the worker bee population. Any factor affecting the queen's fecundity will stagnate colony development, increasing its susceptibility to opportunistic pathogens. We discovered a pathology affecting the ovaries, characterized by a yellow discoloration concentrated in the apex of the ovaries resulting from degenerative lesions in the follicles. In extreme cases, marked by intense discoloration, the majority of the ovarioles were affected and these cases were universally associated with egg-laying deficiencies in the queens. Microscopic examination of the degenerated follicles showed extensive paracrystal lattices of 30 nm icosahedral viral particles. A cDNA library from degenerated ovaries contained a high frequency of deformed wing virus (DWV) and Varroa destructor virus 1 (VDV-1) sequences, two common and closely related honeybee Iflaviruses. These could also be identified by in situ hybridization in various parts of the ovary. A large-scale survey for 10 distinct honeybee viruses showed that DWV and VDV-1 were by far the most prevalent honeybee viruses in queen populations, with distinctly higher prevalence in mated queens (100% and 67%, respectively for DWV and VDV-1) than in virgin queens (37% and 0%, respectively). Since very high viral titres could be recorded in the ovaries and abdomens of both functional and deficient queens, no significant correlation could be made between viral titre and ovarian degeneration or egg-laying deficiency among the wider population of queens. Although our data suggest that DWV and VDV-1 have a role in extreme cases of ovarian degeneration, infection of the ovaries by these viruses does not necessarily result in ovarian degeneration, even at high titres, and additional factors are likely to be involved in this pathology.Laurent GauthierMarc RavallecMagali TournaireFrançois CousseransMax BergoinBenjamin DainatJoachim R de MirandaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 1, p e16217 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Laurent Gauthier Marc Ravallec Magali Tournaire François Cousserans Max Bergoin Benjamin Dainat Joachim R de Miranda Viruses associated with ovarian degeneration in Apis mellifera L. queens.
description	Queen fecundity is a critical issue for the health of honeybee (Apis mellifera L.) colonies, as she is the only reproductive female in the colony and responsible for the constant renewal of the worker bee population. Any factor affecting the queen's fecundity will stagnate colony development, increasing its susceptibility to opportunistic pathogens. We discovered a pathology affecting the ovaries, characterized by a yellow discoloration concentrated in the apex of the ovaries resulting from degenerative lesions in the follicles. In extreme cases, marked by intense discoloration, the majority of the ovarioles were affected and these cases were universally associated with egg-laying deficiencies in the queens. Microscopic examination of the degenerated follicles showed extensive paracrystal lattices of 30 nm icosahedral viral particles. A cDNA library from degenerated ovaries contained a high frequency of deformed wing virus (DWV) and Varroa destructor virus 1 (VDV-1) sequences, two common and closely related honeybee Iflaviruses. These could also be identified by in situ hybridization in various parts of the ovary. A large-scale survey for 10 distinct honeybee viruses showed that DWV and VDV-1 were by far the most prevalent honeybee viruses in queen populations, with distinctly higher prevalence in mated queens (100% and 67%, respectively for DWV and VDV-1) than in virgin queens (37% and 0%, respectively). Since very high viral titres could be recorded in the ovaries and abdomens of both functional and deficient queens, no significant correlation could be made between viral titre and ovarian degeneration or egg-laying deficiency among the wider population of queens. Although our data suggest that DWV and VDV-1 have a role in extreme cases of ovarian degeneration, infection of the ovaries by these viruses does not necessarily result in ovarian degeneration, even at high titres, and additional factors are likely to be involved in this pathology.
format	article
author	Laurent Gauthier Marc Ravallec Magali Tournaire François Cousserans Max Bergoin Benjamin Dainat Joachim R de Miranda
author_facet	Laurent Gauthier Marc Ravallec Magali Tournaire François Cousserans Max Bergoin Benjamin Dainat Joachim R de Miranda
author_sort	Laurent Gauthier
title	Viruses associated with ovarian degeneration in Apis mellifera L. queens.
title_short	Viruses associated with ovarian degeneration in Apis mellifera L. queens.
title_full	Viruses associated with ovarian degeneration in Apis mellifera L. queens.
title_fullStr	Viruses associated with ovarian degeneration in Apis mellifera L. queens.
title_full_unstemmed	Viruses associated with ovarian degeneration in Apis mellifera L. queens.
title_sort	viruses associated with ovarian degeneration in apis mellifera l. queens.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/7dd4ed6023654021be9db5a89bcdd5e9
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Viruses associated with ovarian degeneration in Apis mellifera L. queens.

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