Transcriptome analysis of wheat spikes in response to Tilletia controversa Kühn which cause wheat dwarf bunt

Abstract Wheat dwarf bunt is caused by Tilletia controversa Kühn, which is one of the most destructive diseases of wheat worldwide. To explore the interaction of T. controversa and wheat, we analysed the transcriptome profile of spikes of the susceptible wheat cultivar Dongxuan 3, which was subjecte...

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Autores principales:	Zhaoyu Ren, Jianjian Liu, Ghulam Muhae Ud Din, Han Zhang, Zhenzhen Du, Wanquan Chen, Taiguo Liu, Jianmin Zhang, Sifeng Zhao, Li Gao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7ddeeeca441742f28737f7895f6f6e00
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spelling	oai:doaj.org-article:7ddeeeca441742f28737f7895f6f6e002021-12-02T11:43:43ZTranscriptome analysis of wheat spikes in response to Tilletia controversa Kühn which cause wheat dwarf bunt10.1038/s41598-020-78628-02045-2322https://doaj.org/article/7ddeeeca441742f28737f7895f6f6e002020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78628-0https://doaj.org/toc/2045-2322Abstract Wheat dwarf bunt is caused by Tilletia controversa Kühn, which is one of the most destructive diseases of wheat worldwide. To explore the interaction of T. controversa and wheat, we analysed the transcriptome profile of spikes of the susceptible wheat cultivar Dongxuan 3, which was subjected to a T. controversa infection and a mock infection. The results obtained from a differential expression analysis of T. controversa-infected plants compared with mock-infected ones showed that 10,867 out of 21,354 genes were upregulated, while 10,487 genes were downregulated, and these genes were enriched in 205 different pathways. Our findings demonstrated that the genes associated with defence against diseases, such as PR-related genes, WRKY transcription factors and mitogen-activated protein kinase genes, were more highly expressed in response to T. controversa infection. Additionally, a number of genes related to physiological attributes were expressed during infection. Three pathways were differentiated based on the characteristics of gene ontology classification. KEGG enrichment analysis showed that twenty genes were expressed differentially during the infection of wheat with T. controversa. Notable changes were observed in the transcriptomes of wheat plants after infection. The results of this study may help to elucidate the mechanism governing the interactions between this pathogen and wheat plants and may facilitate the development of new methods to increase the resistance level of wheat against T. controversa, including the overexpression of defence-related genes.Zhaoyu RenJianjian LiuGhulam Muhae Ud DinHan ZhangZhenzhen DuWanquan ChenTaiguo LiuJianmin ZhangSifeng ZhaoLi GaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhaoyu Ren Jianjian Liu Ghulam Muhae Ud Din Han Zhang Zhenzhen Du Wanquan Chen Taiguo Liu Jianmin Zhang Sifeng Zhao Li Gao Transcriptome analysis of wheat spikes in response to Tilletia controversa Kühn which cause wheat dwarf bunt
description	Abstract Wheat dwarf bunt is caused by Tilletia controversa Kühn, which is one of the most destructive diseases of wheat worldwide. To explore the interaction of T. controversa and wheat, we analysed the transcriptome profile of spikes of the susceptible wheat cultivar Dongxuan 3, which was subjected to a T. controversa infection and a mock infection. The results obtained from a differential expression analysis of T. controversa-infected plants compared with mock-infected ones showed that 10,867 out of 21,354 genes were upregulated, while 10,487 genes were downregulated, and these genes were enriched in 205 different pathways. Our findings demonstrated that the genes associated with defence against diseases, such as PR-related genes, WRKY transcription factors and mitogen-activated protein kinase genes, were more highly expressed in response to T. controversa infection. Additionally, a number of genes related to physiological attributes were expressed during infection. Three pathways were differentiated based on the characteristics of gene ontology classification. KEGG enrichment analysis showed that twenty genes were expressed differentially during the infection of wheat with T. controversa. Notable changes were observed in the transcriptomes of wheat plants after infection. The results of this study may help to elucidate the mechanism governing the interactions between this pathogen and wheat plants and may facilitate the development of new methods to increase the resistance level of wheat against T. controversa, including the overexpression of defence-related genes.
format	article
author	Zhaoyu Ren Jianjian Liu Ghulam Muhae Ud Din Han Zhang Zhenzhen Du Wanquan Chen Taiguo Liu Jianmin Zhang Sifeng Zhao Li Gao
author_facet	Zhaoyu Ren Jianjian Liu Ghulam Muhae Ud Din Han Zhang Zhenzhen Du Wanquan Chen Taiguo Liu Jianmin Zhang Sifeng Zhao Li Gao
author_sort	Zhaoyu Ren
title	Transcriptome analysis of wheat spikes in response to Tilletia controversa Kühn which cause wheat dwarf bunt
title_short	Transcriptome analysis of wheat spikes in response to Tilletia controversa Kühn which cause wheat dwarf bunt
title_full	Transcriptome analysis of wheat spikes in response to Tilletia controversa Kühn which cause wheat dwarf bunt
title_fullStr	Transcriptome analysis of wheat spikes in response to Tilletia controversa Kühn which cause wheat dwarf bunt
title_full_unstemmed	Transcriptome analysis of wheat spikes in response to Tilletia controversa Kühn which cause wheat dwarf bunt
title_sort	transcriptome analysis of wheat spikes in response to tilletia controversa kühn which cause wheat dwarf bunt
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/7ddeeeca441742f28737f7895f6f6e00
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Transcriptome analysis of wheat spikes in response to Tilletia controversa Kühn which cause wheat dwarf bunt

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