Genome-wide identification, characteristics and expression of the prolamin genes in Thinopyrum elongatum

Abstract Background Prolamins, unique to Gramineae (grasses), play a key role in the human diet. Thinopyrum elongatum (syn. Agropyron elongatum or Lophopyrum elongatum), a grass of the Triticeae family with a diploid E genome (2n = 2x = 14), is genetically well-characterized, but little is known abo...

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Autores principales:	Wenyang Ge, Yu Gao, Shoushen Xu, Xin Ma, Hongwei Wang, Lingrang Kong, Silong Sun
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Thinopyrum elongatum Prolamins Expression Evolution Celiac disease Biotechnology TP248.13-248.65 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/7ddf8d64759845d3adc90109eb67e669
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spelling	oai:doaj.org-article:7ddf8d64759845d3adc90109eb67e6692021-12-05T12:17:13ZGenome-wide identification, characteristics and expression of the prolamin genes in Thinopyrum elongatum10.1186/s12864-021-08088-x1471-2164https://doaj.org/article/7ddf8d64759845d3adc90109eb67e6692021-12-01T00:00:00Zhttps://doi.org/10.1186/s12864-021-08088-xhttps://doaj.org/toc/1471-2164Abstract Background Prolamins, unique to Gramineae (grasses), play a key role in the human diet. Thinopyrum elongatum (syn. Agropyron elongatum or Lophopyrum elongatum), a grass of the Triticeae family with a diploid E genome (2n = 2x = 14), is genetically well-characterized, but little is known about its prolamin genes and the relationships with homologous loci in the Triticeae species. Results In this study, a total of 19 α-gliadin, 9 γ-gliadin, 19 ω-gliadin, 2 high-molecular-weight glutenin subunit (HMW-GS), and 5 low-molecular-weight glutenin subunit (LMW-GS) genes were identified in the Th. elongatum genome. Micro-synteny and phylogenetic analysis revealed dynamic changes of prolamin gene regions and genetic affinities among Th. elongatum, Triticum aestivum, T. urartu and Aegilops tauschii. The Th. elongatum genome, like the B subgenome of T. aestivum, only contained celiac disease epitope DQ8-glia-α1/DQ8.5-glia-α1, which provided a theoretical basis for the low gluten toxicity wheat breeding. The transcriptome data of Th. elongatum exhibited differential expression in quantity and pattern in the same subfamily or different subfamilies. Dough rheological properties of T. aestivum-Th. elongatum disomic substitution (DS) line 1E(1D) showed higher peak height values than that of their parents, and DS6E(6D) exhibited fewer α-gliadins, which indicates the potential usage for wheat quality breeding. Conclusions Overall, this study provided a comprehensive overview of the prolamin gene family in Th. elongatum, and suggested a promising use of this species in the generation of improved wheat breeds intended for the human diet.Wenyang GeYu GaoShoushen XuXin MaHongwei WangLingrang KongSilong SunBMCarticleThinopyrum elongatumProlaminsExpressionEvolutionCeliac diseaseBiotechnologyTP248.13-248.65GeneticsQH426-470ENBMC Genomics, Vol 22, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Thinopyrum elongatum Prolamins Expression Evolution Celiac disease Biotechnology TP248.13-248.65 Genetics QH426-470
spellingShingle	Thinopyrum elongatum Prolamins Expression Evolution Celiac disease Biotechnology TP248.13-248.65 Genetics QH426-470 Wenyang Ge Yu Gao Shoushen Xu Xin Ma Hongwei Wang Lingrang Kong Silong Sun Genome-wide identification, characteristics and expression of the prolamin genes in Thinopyrum elongatum
description	Abstract Background Prolamins, unique to Gramineae (grasses), play a key role in the human diet. Thinopyrum elongatum (syn. Agropyron elongatum or Lophopyrum elongatum), a grass of the Triticeae family with a diploid E genome (2n = 2x = 14), is genetically well-characterized, but little is known about its prolamin genes and the relationships with homologous loci in the Triticeae species. Results In this study, a total of 19 α-gliadin, 9 γ-gliadin, 19 ω-gliadin, 2 high-molecular-weight glutenin subunit (HMW-GS), and 5 low-molecular-weight glutenin subunit (LMW-GS) genes were identified in the Th. elongatum genome. Micro-synteny and phylogenetic analysis revealed dynamic changes of prolamin gene regions and genetic affinities among Th. elongatum, Triticum aestivum, T. urartu and Aegilops tauschii. The Th. elongatum genome, like the B subgenome of T. aestivum, only contained celiac disease epitope DQ8-glia-α1/DQ8.5-glia-α1, which provided a theoretical basis for the low gluten toxicity wheat breeding. The transcriptome data of Th. elongatum exhibited differential expression in quantity and pattern in the same subfamily or different subfamilies. Dough rheological properties of T. aestivum-Th. elongatum disomic substitution (DS) line 1E(1D) showed higher peak height values than that of their parents, and DS6E(6D) exhibited fewer α-gliadins, which indicates the potential usage for wheat quality breeding. Conclusions Overall, this study provided a comprehensive overview of the prolamin gene family in Th. elongatum, and suggested a promising use of this species in the generation of improved wheat breeds intended for the human diet.
format	article
author	Wenyang Ge Yu Gao Shoushen Xu Xin Ma Hongwei Wang Lingrang Kong Silong Sun
author_facet	Wenyang Ge Yu Gao Shoushen Xu Xin Ma Hongwei Wang Lingrang Kong Silong Sun
author_sort	Wenyang Ge
title	Genome-wide identification, characteristics and expression of the prolamin genes in Thinopyrum elongatum
title_short	Genome-wide identification, characteristics and expression of the prolamin genes in Thinopyrum elongatum
title_full	Genome-wide identification, characteristics and expression of the prolamin genes in Thinopyrum elongatum
title_fullStr	Genome-wide identification, characteristics and expression of the prolamin genes in Thinopyrum elongatum
title_full_unstemmed	Genome-wide identification, characteristics and expression of the prolamin genes in Thinopyrum elongatum
title_sort	genome-wide identification, characteristics and expression of the prolamin genes in thinopyrum elongatum
publisher	BMC
publishDate	2021
url	https://doaj.org/article/7ddf8d64759845d3adc90109eb67e669
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Genome-wide identification, characteristics and expression of the prolamin genes in Thinopyrum elongatum

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