Longevity and germination of Juniperus communis L. pollen after storage

Abstract Pollen storage belongs among the most important activities associated with pollen handling. It overcomes the differences in pollen shedding and ovule receptivity during controlled pollination experiments. It is especially important for species like common juniper (Juniperus communis L.) wit...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Andrej Kormuťák, Peter Bolecek, Martin Galgóci, Dušan Gömöry
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7de4cbb5e49f48108bbbe0a87adce3b6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7de4cbb5e49f48108bbbe0a87adce3b6
record_format	dspace
spelling	oai:doaj.org-article:7de4cbb5e49f48108bbbe0a87adce3b62021-12-02T16:04:36ZLongevity and germination of Juniperus communis L. pollen after storage10.1038/s41598-021-90942-92045-2322https://doaj.org/article/7de4cbb5e49f48108bbbe0a87adce3b62021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90942-9https://doaj.org/toc/2045-2322Abstract Pollen storage belongs among the most important activities associated with pollen handling. It overcomes the differences in pollen shedding and ovule receptivity during controlled pollination experiments. It is especially important for species like common juniper (Juniperus communis L.) with an extremely low quality of seeds due to pollination failure. Additionally, it is a substantial part of germplasm preservation programmes in pollen banks. In the present paper, the effect of short-term storage of pollen was studied using pollen samples from five shrubs in an in vitro germination test. Two temperature regimes were tested. The pollen viability of freshly collected pollen varied considerably between individual shrubs, exhibiting 67.3–88.6% germination rate and 248.0–367.3 µm of pollen tubes. Storage at + 4 °C for four months was accompanied by a profound decline in pollen viability. The germination percentage was reduced to 49.2–75.2% and the pollen tube length to 32.5–69.0%, depending on individual shrubs. The corresponding decline in pollen viability characteristics during storage at − 20 °C was only negligible in two of the tested shrubs. In the remaining three shrub samples, an increase in germination percentage was observed. Pollen tube growth responded more sensitively to freezing, but, on average, the decrease in length was lower than that at + 4 °C. The rate of reduction in pollen tube length varied between 11.5 and 45.4%. Cytological events accompanying in vitro germination of freezer-stored pollen exhibited some delay in releasing the exine from pollen grains during the early stages of germination as compared with freshly collected pollen. In conclusion, short-term storage of the common juniper pollen in a freezer is better for the preservation of its viability than storage at + 4 °C.Andrej KormuťákPeter BolecekMartin GalgóciDušan GömöryNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Andrej Kormuťák Peter Bolecek Martin Galgóci Dušan Gömöry Longevity and germination of Juniperus communis L. pollen after storage
description	Abstract Pollen storage belongs among the most important activities associated with pollen handling. It overcomes the differences in pollen shedding and ovule receptivity during controlled pollination experiments. It is especially important for species like common juniper (Juniperus communis L.) with an extremely low quality of seeds due to pollination failure. Additionally, it is a substantial part of germplasm preservation programmes in pollen banks. In the present paper, the effect of short-term storage of pollen was studied using pollen samples from five shrubs in an in vitro germination test. Two temperature regimes were tested. The pollen viability of freshly collected pollen varied considerably between individual shrubs, exhibiting 67.3–88.6% germination rate and 248.0–367.3 µm of pollen tubes. Storage at + 4 °C for four months was accompanied by a profound decline in pollen viability. The germination percentage was reduced to 49.2–75.2% and the pollen tube length to 32.5–69.0%, depending on individual shrubs. The corresponding decline in pollen viability characteristics during storage at − 20 °C was only negligible in two of the tested shrubs. In the remaining three shrub samples, an increase in germination percentage was observed. Pollen tube growth responded more sensitively to freezing, but, on average, the decrease in length was lower than that at + 4 °C. The rate of reduction in pollen tube length varied between 11.5 and 45.4%. Cytological events accompanying in vitro germination of freezer-stored pollen exhibited some delay in releasing the exine from pollen grains during the early stages of germination as compared with freshly collected pollen. In conclusion, short-term storage of the common juniper pollen in a freezer is better for the preservation of its viability than storage at + 4 °C.
format	article
author	Andrej Kormuťák Peter Bolecek Martin Galgóci Dušan Gömöry
author_facet	Andrej Kormuťák Peter Bolecek Martin Galgóci Dušan Gömöry
author_sort	Andrej Kormuťák
title	Longevity and germination of Juniperus communis L. pollen after storage
title_short	Longevity and germination of Juniperus communis L. pollen after storage
title_full	Longevity and germination of Juniperus communis L. pollen after storage
title_fullStr	Longevity and germination of Juniperus communis L. pollen after storage
title_full_unstemmed	Longevity and germination of Juniperus communis L. pollen after storage
title_sort	longevity and germination of juniperus communis l. pollen after storage
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7de4cbb5e49f48108bbbe0a87adce3b6
work_keys_str_mv	AT andrejkormutak longevityandgerminationofjuniperuscommunislpollenafterstorage AT peterbolecek longevityandgerminationofjuniperuscommunislpollenafterstorage AT martingalgoci longevityandgerminationofjuniperuscommunislpollenafterstorage AT dusangomory longevityandgerminationofjuniperuscommunislpollenafterstorage
_version_	1718385216141131776

Longevity and germination of Juniperus communis L. pollen after storage

Ejemplares similares