The suitability of NONO-TFE3 dual-fusion FISH assay as a diagnostic tool for NONO-TFE3 renal cell carcinoma

The suitability of NONO-TFE3 dual-fusion FISH assay as a diagnostic tool for NONO-TFE3 renal cell carcinoma

Abstract NONO-TFE3 RCC is a subtype of Xp11.2 translocation renal cell carcinoma (RCC). So far, only a small amount of NONO-TFE3 RCC have been reported owing to lack of effective diagnosis methods. Utilizing the novel dual-fusion fluorescence in situ hybridization (FISH) probe reported here, 5 cases...

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Autores principales: Ning Liu, Wei Guo, Qiancheng Shi, Wenyuan Zhuang, Xiaohong Pu, Shaoyu Chen, Feng Qu, Linfeng Xu, Xiaozhi Zhao, Xiaogong Li, Gutian Zhang, Hongqian Guo, Weidong Gan, Dongmei Li
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Publicado: Nature Portfolio 2020
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spelling oai:doaj.org-article:7de7a6e77fd04ffa9a0bbb33ad502b3d2021-12-02T18:51:27ZThe suitability of NONO-TFE3 dual-fusion FISH assay as a diagnostic tool for NONO-TFE3 renal cell carcinoma10.1038/s41598-020-73309-42045-2322https://doaj.org/article/7de7a6e77fd04ffa9a0bbb33ad502b3d2020-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-73309-4https://doaj.org/toc/2045-2322Abstract NONO-TFE3 RCC is a subtype of Xp11.2 translocation renal cell carcinoma (RCC). So far, only a small amount of NONO-TFE3 RCC have been reported owing to lack of effective diagnosis methods. Utilizing the novel dual-fusion fluorescence in situ hybridization (FISH) probe reported here, 5 cases of NONO-TFE3 RCC were identified and were ultimately confirmed by RT-PCR. Histopathology, all 5 cases were consisted by sheets of epithelial cells and papillary architecture. The cytoplasm was abundantly clear, and nucleoli was not prominent. Besides, the nuclear palisading, subnuclear vacuoles and psammoma bodies were identified. The most distinctive features were strong positive TFE3 staining but equivocal split signals of the TFE3 probe, which might lead to the misdiagnosis of Xp11.2 translocation RCC. The median age and median tumor size of the five patients were 41.2 years and 3.6 cm, respectively. A median following follow-up of 27 months showed moderate disease progression and prognosis in NONO-TFE3 RCC patients. In conclusion, the present study demonstrates the effectiveness and reliability of the NONO-TFE3 dual-fusion FISH probe for diagnosing NONO-TFE3 RCC. Suspected cases of Xp11.2 translocation RCC showing biphasic pattern, strong positive TFE3 staining, and equivocal split signals in the TFE3 FISH assay indicated a possibility of NONO-TFE3 RCC.Ning LiuWei GuoQiancheng ShiWenyuan ZhuangXiaohong PuShaoyu ChenFeng QuLinfeng XuXiaozhi ZhaoXiaogong LiGutian ZhangHongqian GuoWeidong GanDongmei LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Ning Liu
Wei Guo
Qiancheng Shi
Wenyuan Zhuang
Xiaohong Pu
Shaoyu Chen
Feng Qu
Linfeng Xu
Xiaozhi Zhao
Xiaogong Li
Gutian Zhang
Hongqian Guo
Weidong Gan
Dongmei Li
The suitability of NONO-TFE3 dual-fusion FISH assay as a diagnostic tool for NONO-TFE3 renal cell carcinoma
description Abstract NONO-TFE3 RCC is a subtype of Xp11.2 translocation renal cell carcinoma (RCC). So far, only a small amount of NONO-TFE3 RCC have been reported owing to lack of effective diagnosis methods. Utilizing the novel dual-fusion fluorescence in situ hybridization (FISH) probe reported here, 5 cases of NONO-TFE3 RCC were identified and were ultimately confirmed by RT-PCR. Histopathology, all 5 cases were consisted by sheets of epithelial cells and papillary architecture. The cytoplasm was abundantly clear, and nucleoli was not prominent. Besides, the nuclear palisading, subnuclear vacuoles and psammoma bodies were identified. The most distinctive features were strong positive TFE3 staining but equivocal split signals of the TFE3 probe, which might lead to the misdiagnosis of Xp11.2 translocation RCC. The median age and median tumor size of the five patients were 41.2 years and 3.6 cm, respectively. A median following follow-up of 27 months showed moderate disease progression and prognosis in NONO-TFE3 RCC patients. In conclusion, the present study demonstrates the effectiveness and reliability of the NONO-TFE3 dual-fusion FISH probe for diagnosing NONO-TFE3 RCC. Suspected cases of Xp11.2 translocation RCC showing biphasic pattern, strong positive TFE3 staining, and equivocal split signals in the TFE3 FISH assay indicated a possibility of NONO-TFE3 RCC.
format article
author Ning Liu
Wei Guo
Qiancheng Shi
Wenyuan Zhuang
Xiaohong Pu
Shaoyu Chen
Feng Qu
Linfeng Xu
Xiaozhi Zhao
Xiaogong Li
Gutian Zhang
Hongqian Guo
Weidong Gan
Dongmei Li
author_facet Ning Liu
Wei Guo
Qiancheng Shi
Wenyuan Zhuang
Xiaohong Pu
Shaoyu Chen
Feng Qu
Linfeng Xu
Xiaozhi Zhao
Xiaogong Li
Gutian Zhang
Hongqian Guo
Weidong Gan
Dongmei Li
author_sort Ning Liu
title The suitability of NONO-TFE3 dual-fusion FISH assay as a diagnostic tool for NONO-TFE3 renal cell carcinoma
title_short The suitability of NONO-TFE3 dual-fusion FISH assay as a diagnostic tool for NONO-TFE3 renal cell carcinoma
title_full The suitability of NONO-TFE3 dual-fusion FISH assay as a diagnostic tool for NONO-TFE3 renal cell carcinoma
title_fullStr The suitability of NONO-TFE3 dual-fusion FISH assay as a diagnostic tool for NONO-TFE3 renal cell carcinoma
title_full_unstemmed The suitability of NONO-TFE3 dual-fusion FISH assay as a diagnostic tool for NONO-TFE3 renal cell carcinoma
title_sort suitability of nono-tfe3 dual-fusion fish assay as a diagnostic tool for nono-tfe3 renal cell carcinoma
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/7de7a6e77fd04ffa9a0bbb33ad502b3d
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