JKAP relates to disease risk, severity, and Th1 and Th17 differentiation in Parkinson's disease

Abstract Objective JNK pathway‐associated phosphatase (JKAP) is previously reported to regulate immune/inflammatory process via T‐cell signaling, and closely involves in neurological diseases, while its implication in Parkinson's disease (PD) is unknown. Therefore, this study aimed to investiga...

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Autores principales:	Qingwei Yang, Jingcong Zhuang, Pingping Cai, Longling Li, Rong Wang, Zhongjie Chen
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/7de7c38244e44b79a65f18954214a270
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spelling	oai:doaj.org-article:7de7c38244e44b79a65f18954214a2702021-11-19T13:55:31ZJKAP relates to disease risk, severity, and Th1 and Th17 differentiation in Parkinson's disease2328-950310.1002/acn3.51420https://doaj.org/article/7de7c38244e44b79a65f18954214a2702021-09-01T00:00:00Zhttps://doi.org/10.1002/acn3.51420https://doaj.org/toc/2328-9503Abstract Objective JNK pathway‐associated phosphatase (JKAP) is previously reported to regulate immune/inflammatory process via T‐cell signaling, and closely involves in neurological diseases, while its implication in Parkinson's disease (PD) is unknown. Therefore, this study aimed to investigate the correlation of JKAP with Th1/Th2/Th17 cells and their clinical roles in PD patients, and then further explore the effect of JKAP on regulating CD4+ T‐cell differentiation in PD. Methods Totally 50 PD patients and 50 age‐/gender‐matched controls were enrolled. Their blood samples were collected and proposed to ELISA and flow cytometry assays for JKAP, Th1, Th2, and Th17 measurements. In vitro, CD4+ T cells were isolated from PD patients then transfected with JKAP overexpression and knockdown Lentivirus, followed by detection of markers (CD25+ cell proportion, CD69+ cell proportion, IFN‐γ, IL10, and IL17). Results JKAP was downregulated in PD patients compared to controls, which also showed good potency to discriminate them. Besides, JKAP negatively correlated with Th1 and Th17 cell proportions, but did not associate with Th2 cell proportion in PD patients; Interestingly, JKAP did not correlated with Th1, Th2, or Th17 cell proportions in controls. Furthermore, JKAP correlated with some parts of unified Parkinson’s Disease Rating Scale (UPDRS) and Mini‐Mental State Examination (MMSE) score. In vitro, JKAP overexpression repressed CD4+ T‐cell activation and its differentiation into Th1 and Th17 cells in PD, while JKAP knockdown appeared opposite effect. Interpretation JKAP associates with disease risk and severity, correlates with Th1 and Th17 cells, and regulates CD4+ T‐cell activation/differentiation in PD.Qingwei YangJingcong ZhuangPingping CaiLongling LiRong WangZhongjie ChenWileyarticleNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENAnnals of Clinical and Translational Neurology, Vol 8, Iss 9, Pp 1786-1795 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Qingwei Yang Jingcong Zhuang Pingping Cai Longling Li Rong Wang Zhongjie Chen JKAP relates to disease risk, severity, and Th1 and Th17 differentiation in Parkinson's disease
description	Abstract Objective JNK pathway‐associated phosphatase (JKAP) is previously reported to regulate immune/inflammatory process via T‐cell signaling, and closely involves in neurological diseases, while its implication in Parkinson's disease (PD) is unknown. Therefore, this study aimed to investigate the correlation of JKAP with Th1/Th2/Th17 cells and their clinical roles in PD patients, and then further explore the effect of JKAP on regulating CD4+ T‐cell differentiation in PD. Methods Totally 50 PD patients and 50 age‐/gender‐matched controls were enrolled. Their blood samples were collected and proposed to ELISA and flow cytometry assays for JKAP, Th1, Th2, and Th17 measurements. In vitro, CD4+ T cells were isolated from PD patients then transfected with JKAP overexpression and knockdown Lentivirus, followed by detection of markers (CD25+ cell proportion, CD69+ cell proportion, IFN‐γ, IL10, and IL17). Results JKAP was downregulated in PD patients compared to controls, which also showed good potency to discriminate them. Besides, JKAP negatively correlated with Th1 and Th17 cell proportions, but did not associate with Th2 cell proportion in PD patients; Interestingly, JKAP did not correlated with Th1, Th2, or Th17 cell proportions in controls. Furthermore, JKAP correlated with some parts of unified Parkinson’s Disease Rating Scale (UPDRS) and Mini‐Mental State Examination (MMSE) score. In vitro, JKAP overexpression repressed CD4+ T‐cell activation and its differentiation into Th1 and Th17 cells in PD, while JKAP knockdown appeared opposite effect. Interpretation JKAP associates with disease risk and severity, correlates with Th1 and Th17 cells, and regulates CD4+ T‐cell activation/differentiation in PD.
format	article
author	Qingwei Yang Jingcong Zhuang Pingping Cai Longling Li Rong Wang Zhongjie Chen
author_facet	Qingwei Yang Jingcong Zhuang Pingping Cai Longling Li Rong Wang Zhongjie Chen
author_sort	Qingwei Yang
title	JKAP relates to disease risk, severity, and Th1 and Th17 differentiation in Parkinson's disease
title_short	JKAP relates to disease risk, severity, and Th1 and Th17 differentiation in Parkinson's disease
title_full	JKAP relates to disease risk, severity, and Th1 and Th17 differentiation in Parkinson's disease
title_fullStr	JKAP relates to disease risk, severity, and Th1 and Th17 differentiation in Parkinson's disease
title_full_unstemmed	JKAP relates to disease risk, severity, and Th1 and Th17 differentiation in Parkinson's disease
title_sort	jkap relates to disease risk, severity, and th1 and th17 differentiation in parkinson's disease
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/7de7c38244e44b79a65f18954214a270
work_keys_str_mv	AT qingweiyang jkaprelatestodiseaseriskseverityandth1andth17differentiationinparkinsonsdisease AT jingcongzhuang jkaprelatestodiseaseriskseverityandth1andth17differentiationinparkinsonsdisease AT pingpingcai jkaprelatestodiseaseriskseverityandth1andth17differentiationinparkinsonsdisease AT longlingli jkaprelatestodiseaseriskseverityandth1andth17differentiationinparkinsonsdisease AT rongwang jkaprelatestodiseaseriskseverityandth1andth17differentiationinparkinsonsdisease AT zhongjiechen jkaprelatestodiseaseriskseverityandth1andth17differentiationinparkinsonsdisease
_version_	1718420077328465920

JKAP relates to disease risk, severity, and Th1 and Th17 differentiation in Parkinson's disease

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