Curcumin inhibited hepatitis B viral entry through NTCP binding

Abstract Hepatitis B virus (HBV) has been implicated in hepatitis and hepatocellular carcinoma. Current agents (nucleos(t)ide analogs and interferons) could only attenuate HBV infection. A combination of agents targeting different stages of viral life cycle (e.g., entry, replication, and cccDNA stab...

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Autores principales:	Piyanoot Thongsri, Yongyut Pewkliang, Suparerk Borwornpinyo, Adisak Wongkajornsilp, Suradej Hongeng, Khanit Sa-ngiamsuntorn
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7dea2b7df0b440859e7298ba57146554
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spelling	oai:doaj.org-article:7dea2b7df0b440859e7298ba571465542021-12-02T17:17:40ZCurcumin inhibited hepatitis B viral entry through NTCP binding10.1038/s41598-021-98243-x2045-2322https://doaj.org/article/7dea2b7df0b440859e7298ba571465542021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98243-xhttps://doaj.org/toc/2045-2322Abstract Hepatitis B virus (HBV) has been implicated in hepatitis and hepatocellular carcinoma. Current agents (nucleos(t)ide analogs and interferons) could only attenuate HBV infection. A combination of agents targeting different stages of viral life cycle (e.g., entry, replication, and cccDNA stability) was expected to eradicate the infection. Curcumin (CCM) was investigated for inhibitory action toward HBV attachment and internalization. Immortalized hepatocyte-like cells (imHCs), HepaRG and non-hepatic cells served as host cells for binding study with CCM. CCM decreased viral load, HBeAg, HBcAg (infectivity), intracellular HBV DNA, and cccDNA levels. The CCM-induced suppression of HBV entry was directly correlated with the density of sodium-taurocholate co-transporting polypeptide (NTCP), a known host receptor for HBV entry. The site of action of CCM was confirmed using TCA uptake assay. The affinity between CCM and NTCP was measured using isothermal titration calorimetry (ITC). These results demonstrated that CCM interrupted HBV entry and would therefore suppress HBV re-infection.Piyanoot ThongsriYongyut PewkliangSuparerk BorwornpinyoAdisak WongkajornsilpSuradej HongengKhanit Sa-ngiamsuntornNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Piyanoot Thongsri Yongyut Pewkliang Suparerk Borwornpinyo Adisak Wongkajornsilp Suradej Hongeng Khanit Sa-ngiamsuntorn Curcumin inhibited hepatitis B viral entry through NTCP binding
description	Abstract Hepatitis B virus (HBV) has been implicated in hepatitis and hepatocellular carcinoma. Current agents (nucleos(t)ide analogs and interferons) could only attenuate HBV infection. A combination of agents targeting different stages of viral life cycle (e.g., entry, replication, and cccDNA stability) was expected to eradicate the infection. Curcumin (CCM) was investigated for inhibitory action toward HBV attachment and internalization. Immortalized hepatocyte-like cells (imHCs), HepaRG and non-hepatic cells served as host cells for binding study with CCM. CCM decreased viral load, HBeAg, HBcAg (infectivity), intracellular HBV DNA, and cccDNA levels. The CCM-induced suppression of HBV entry was directly correlated with the density of sodium-taurocholate co-transporting polypeptide (NTCP), a known host receptor for HBV entry. The site of action of CCM was confirmed using TCA uptake assay. The affinity between CCM and NTCP was measured using isothermal titration calorimetry (ITC). These results demonstrated that CCM interrupted HBV entry and would therefore suppress HBV re-infection.
format	article
author	Piyanoot Thongsri Yongyut Pewkliang Suparerk Borwornpinyo Adisak Wongkajornsilp Suradej Hongeng Khanit Sa-ngiamsuntorn
author_facet	Piyanoot Thongsri Yongyut Pewkliang Suparerk Borwornpinyo Adisak Wongkajornsilp Suradej Hongeng Khanit Sa-ngiamsuntorn
author_sort	Piyanoot Thongsri
title	Curcumin inhibited hepatitis B viral entry through NTCP binding
title_short	Curcumin inhibited hepatitis B viral entry through NTCP binding
title_full	Curcumin inhibited hepatitis B viral entry through NTCP binding
title_fullStr	Curcumin inhibited hepatitis B viral entry through NTCP binding
title_full_unstemmed	Curcumin inhibited hepatitis B viral entry through NTCP binding
title_sort	curcumin inhibited hepatitis b viral entry through ntcp binding
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7dea2b7df0b440859e7298ba57146554
work_keys_str_mv	AT piyanootthongsri curcumininhibitedhepatitisbviralentrythroughntcpbinding AT yongyutpewkliang curcumininhibitedhepatitisbviralentrythroughntcpbinding AT suparerkborwornpinyo curcumininhibitedhepatitisbviralentrythroughntcpbinding AT adisakwongkajornsilp curcumininhibitedhepatitisbviralentrythroughntcpbinding AT suradejhongeng curcumininhibitedhepatitisbviralentrythroughntcpbinding AT khanitsangiamsuntorn curcumininhibitedhepatitisbviralentrythroughntcpbinding
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Curcumin inhibited hepatitis B viral entry through NTCP binding

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