The glutamatergic neurons in the spinal cord of the sea lamprey: an in situ hybridization and immunohistochemical study.

Glutamate is the main excitatory neurotransmitter involved in spinal cord circuits in vertebrates, but in most groups the distribution of glutamatergic spinal neurons is still unknown. Lampreys have been extensively used as a model to investigate the neuronal circuits underlying locomotion. Glutamat...

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Autores principales:	Blanca Fernández-López, Verona Villar-Cerviño, Silvia M Valle-Maroto, Antón Barreiro-Iglesias, Ramón Anadón, María Celina Rodicio
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7def90f2e62b473582e167c325e5bf4c
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spelling	oai:doaj.org-article:7def90f2e62b473582e167c325e5bf4c2021-11-18T08:11:24ZThe glutamatergic neurons in the spinal cord of the sea lamprey: an in situ hybridization and immunohistochemical study.1932-620310.1371/journal.pone.0047898https://doaj.org/article/7def90f2e62b473582e167c325e5bf4c2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23110124/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Glutamate is the main excitatory neurotransmitter involved in spinal cord circuits in vertebrates, but in most groups the distribution of glutamatergic spinal neurons is still unknown. Lampreys have been extensively used as a model to investigate the neuronal circuits underlying locomotion. Glutamatergic circuits have been characterized on the basis of the excitatory responses elicited in postsynaptic neurons. However, the presence of glutamatergic neurochemical markers in spinal neurons has not been investigated. In this study, we report for the first time the expression of a vesicular glutamate transporter (VGLUT) in the spinal cord of the sea lamprey. We also study the distribution of glutamate in perikarya and fibers. The largest glutamatergic neurons found were the dorsal cells and caudal giant cells. Two additional VGLUT-positive gray matter populations, one dorsomedial consisting of small cells and another one lateral consisting of small and large cells were observed. Some cerebrospinal fluid-contacting cells also expressed VGLUT. In the white matter, some edge cells and some cells associated with giant axons (Müller and Mauthner axons) and the dorsolateral funiculus expressed VGLUT. Large lateral cells and the cells associated with reticulospinal axons are in a key position to receive descending inputs involved in the control of locomotion. We also compared the distribution of glutamate immunoreactivity with that of γ-aminobutyric acid (GABA) and glycine. Colocalization of glutamate and GABA or glycine was observed in some small spinal cells. These results confirm the glutamatergic nature of various neuronal populations, and reveal new small-celled glutamatergic populations, predicting that some glutamatergic neurons would exert complex actions on postsynaptic neurons.Blanca Fernández-LópezVerona Villar-CerviñoSilvia M Valle-MarotoAntón Barreiro-IglesiasRamón AnadónMaría Celina RodicioPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e47898 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Blanca Fernández-López Verona Villar-Cerviño Silvia M Valle-Maroto Antón Barreiro-Iglesias Ramón Anadón María Celina Rodicio The glutamatergic neurons in the spinal cord of the sea lamprey: an in situ hybridization and immunohistochemical study.
description	Glutamate is the main excitatory neurotransmitter involved in spinal cord circuits in vertebrates, but in most groups the distribution of glutamatergic spinal neurons is still unknown. Lampreys have been extensively used as a model to investigate the neuronal circuits underlying locomotion. Glutamatergic circuits have been characterized on the basis of the excitatory responses elicited in postsynaptic neurons. However, the presence of glutamatergic neurochemical markers in spinal neurons has not been investigated. In this study, we report for the first time the expression of a vesicular glutamate transporter (VGLUT) in the spinal cord of the sea lamprey. We also study the distribution of glutamate in perikarya and fibers. The largest glutamatergic neurons found were the dorsal cells and caudal giant cells. Two additional VGLUT-positive gray matter populations, one dorsomedial consisting of small cells and another one lateral consisting of small and large cells were observed. Some cerebrospinal fluid-contacting cells also expressed VGLUT. In the white matter, some edge cells and some cells associated with giant axons (Müller and Mauthner axons) and the dorsolateral funiculus expressed VGLUT. Large lateral cells and the cells associated with reticulospinal axons are in a key position to receive descending inputs involved in the control of locomotion. We also compared the distribution of glutamate immunoreactivity with that of γ-aminobutyric acid (GABA) and glycine. Colocalization of glutamate and GABA or glycine was observed in some small spinal cells. These results confirm the glutamatergic nature of various neuronal populations, and reveal new small-celled glutamatergic populations, predicting that some glutamatergic neurons would exert complex actions on postsynaptic neurons.
format	article
author	Blanca Fernández-López Verona Villar-Cerviño Silvia M Valle-Maroto Antón Barreiro-Iglesias Ramón Anadón María Celina Rodicio
author_facet	Blanca Fernández-López Verona Villar-Cerviño Silvia M Valle-Maroto Antón Barreiro-Iglesias Ramón Anadón María Celina Rodicio
author_sort	Blanca Fernández-López
title	The glutamatergic neurons in the spinal cord of the sea lamprey: an in situ hybridization and immunohistochemical study.
title_short	The glutamatergic neurons in the spinal cord of the sea lamprey: an in situ hybridization and immunohistochemical study.
title_full	The glutamatergic neurons in the spinal cord of the sea lamprey: an in situ hybridization and immunohistochemical study.
title_fullStr	The glutamatergic neurons in the spinal cord of the sea lamprey: an in situ hybridization and immunohistochemical study.
title_full_unstemmed	The glutamatergic neurons in the spinal cord of the sea lamprey: an in situ hybridization and immunohistochemical study.
title_sort	glutamatergic neurons in the spinal cord of the sea lamprey: an in situ hybridization and immunohistochemical study.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/7def90f2e62b473582e167c325e5bf4c
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The glutamatergic neurons in the spinal cord of the sea lamprey: an in situ hybridization and immunohistochemical study.

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