Association of TNF-α Gene Expression and Release in Response to Anti-Diabetic Drugs from Human Adipocytes in vitro
Madhukar Saxena,1,* Daoud Ali,2,* Dinesh Raj Modi,1 Mohammed HA Almarzoug,2 SA Hussain,2 S Manohrdas2 1Department of Biotechnology, Babasaheb Bhimrao Ambedkar University, Lucknow, India; 2Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia*These authors contributed...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Dove Medical Press
2020
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/7df5d0f492014d3abc79500aa9efcffa |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:7df5d0f492014d3abc79500aa9efcffa |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:7df5d0f492014d3abc79500aa9efcffa2021-12-02T08:46:33ZAssociation of TNF-α Gene Expression and Release in Response to Anti-Diabetic Drugs from Human Adipocytes in vitro1178-7007https://doaj.org/article/7df5d0f492014d3abc79500aa9efcffa2020-07-01T00:00:00Zhttps://www.dovepress.com/association-of-tnf-alpha-gene-expression-and-release-in-response-to-an-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Madhukar Saxena,1,* Daoud Ali,2,* Dinesh Raj Modi,1 Mohammed HA Almarzoug,2 SA Hussain,2 S Manohrdas2 1Department of Biotechnology, Babasaheb Bhimrao Ambedkar University, Lucknow, India; 2Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia*These authors contributed equally to this workCorrespondence: Madhukar SaxenaDepartment of Biotechnology, Babasaheb Bhimrao Ambedkar University, Vidya Vihar, Rai Bareilly Road, Lucknow, IndiaEmail madhukarbio@gmail.comIntroduction: TNF-α, a proinflammatory cytokine secreted by activated immune cells, and overexpression of it in adipocytes, has an important role in insulin resistance progression and diabetes development.Aim and Objective: Subcutaneous adipocytes derived from mesenchymal stem cells were used for in vitro analysis to find the role of antidiabetic drugs on TNF-α in high glucose-fed adipocytes.Methods: In vitro adipocytes were used along with variable concentration of anti-diabetic drugs. The level of TNF-α was measured by ELISA and the mRNA level was quantified using SYBR-Green real-time PCR. All data were statistically analyzed.Results: The level of TNF-α and the mRNA expression were observed and analyzed with normal adipocytes. TNF-α level and expression of it showed agonistic behavior, ie no change at low concentration while enhances with the increase of glucose. The level was decreased significantly when the adipocytes were treated with metformin (p=0.015) and pioglitazone (p=0.020). A combination of drugs showed that the expression of TNF-α was almost the same as for metformin alone. However, insulin increases the TNF-α expression as for pioglitazone.Discussion: Such a report on adipocytes may be helpful for clinical benefits to understand the additional mechanism of adipocytes on the release and expression of TNF-α. However, anti-diabetic drugs including insulin up-regulate the TNF-α gene expression in mild or severe glucose load.Keywords: adipocytes, TNF-α, anti-diabetic drugs, T2DM, gene expressionSaxena MAli DModi DRAlmarzoug MHAHussain SAManohrdas SDove Medical Pressarticleadipocytestnf-α;anti-diabetic drugst2dm;gene expressionSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 13, Pp 2633-2640 (2020) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
adipocytes tnf-α;anti-diabetic drugs t2dm;gene expression Specialties of internal medicine RC581-951 |
| spellingShingle |
adipocytes tnf-α;anti-diabetic drugs t2dm;gene expression Specialties of internal medicine RC581-951 Saxena M Ali D Modi DR Almarzoug MHA Hussain SA Manohrdas S Association of TNF-α Gene Expression and Release in Response to Anti-Diabetic Drugs from Human Adipocytes in vitro |
| description |
Madhukar Saxena,1,* Daoud Ali,2,* Dinesh Raj Modi,1 Mohammed HA Almarzoug,2 SA Hussain,2 S Manohrdas2 1Department of Biotechnology, Babasaheb Bhimrao Ambedkar University, Lucknow, India; 2Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia*These authors contributed equally to this workCorrespondence: Madhukar SaxenaDepartment of Biotechnology, Babasaheb Bhimrao Ambedkar University, Vidya Vihar, Rai Bareilly Road, Lucknow, IndiaEmail madhukarbio@gmail.comIntroduction: TNF-α, a proinflammatory cytokine secreted by activated immune cells, and overexpression of it in adipocytes, has an important role in insulin resistance progression and diabetes development.Aim and Objective: Subcutaneous adipocytes derived from mesenchymal stem cells were used for in vitro analysis to find the role of antidiabetic drugs on TNF-α in high glucose-fed adipocytes.Methods: In vitro adipocytes were used along with variable concentration of anti-diabetic drugs. The level of TNF-α was measured by ELISA and the mRNA level was quantified using SYBR-Green real-time PCR. All data were statistically analyzed.Results: The level of TNF-α and the mRNA expression were observed and analyzed with normal adipocytes. TNF-α level and expression of it showed agonistic behavior, ie no change at low concentration while enhances with the increase of glucose. The level was decreased significantly when the adipocytes were treated with metformin (p=0.015) and pioglitazone (p=0.020). A combination of drugs showed that the expression of TNF-α was almost the same as for metformin alone. However, insulin increases the TNF-α expression as for pioglitazone.Discussion: Such a report on adipocytes may be helpful for clinical benefits to understand the additional mechanism of adipocytes on the release and expression of TNF-α. However, anti-diabetic drugs including insulin up-regulate the TNF-α gene expression in mild or severe glucose load.Keywords: adipocytes, TNF-α, anti-diabetic drugs, T2DM, gene expression |
| format |
article |
| author |
Saxena M Ali D Modi DR Almarzoug MHA Hussain SA Manohrdas S |
| author_facet |
Saxena M Ali D Modi DR Almarzoug MHA Hussain SA Manohrdas S |
| author_sort |
Saxena M |
| title |
Association of TNF-α Gene Expression and Release in Response to Anti-Diabetic Drugs from Human Adipocytes in vitro |
| title_short |
Association of TNF-α Gene Expression and Release in Response to Anti-Diabetic Drugs from Human Adipocytes in vitro |
| title_full |
Association of TNF-α Gene Expression and Release in Response to Anti-Diabetic Drugs from Human Adipocytes in vitro |
| title_fullStr |
Association of TNF-α Gene Expression and Release in Response to Anti-Diabetic Drugs from Human Adipocytes in vitro |
| title_full_unstemmed |
Association of TNF-α Gene Expression and Release in Response to Anti-Diabetic Drugs from Human Adipocytes in vitro |
| title_sort |
association of tnf-α gene expression and release in response to anti-diabetic drugs from human adipocytes in vitro |
| publisher |
Dove Medical Press |
| publishDate |
2020 |
| url |
https://doaj.org/article/7df5d0f492014d3abc79500aa9efcffa |
| work_keys_str_mv |
AT saxenam associationoftnfalphageneexpressionandreleaseinresponsetoantidiabeticdrugsfromhumanadipocytesinvitro AT alid associationoftnfalphageneexpressionandreleaseinresponsetoantidiabeticdrugsfromhumanadipocytesinvitro AT modidr associationoftnfalphageneexpressionandreleaseinresponsetoantidiabeticdrugsfromhumanadipocytesinvitro AT almarzougmha associationoftnfalphageneexpressionandreleaseinresponsetoantidiabeticdrugsfromhumanadipocytesinvitro AT hussainsa associationoftnfalphageneexpressionandreleaseinresponsetoantidiabeticdrugsfromhumanadipocytesinvitro AT manohrdass associationoftnfalphageneexpressionandreleaseinresponsetoantidiabeticdrugsfromhumanadipocytesinvitro |
| _version_ |
1718398372644126720 |