Draft genome sequence of a carbapenem-resistant clinical Acinetobacter baumannii revealing co-existence of four classes of β-lactamases

ABSTRACT: Objectives: Nosocomial carbapenem-resistant Acinetobacter baumannii is a challenge in the treatment of intensive care unit (ICU) patients. The presence of antimicrobial resistance genes (ARGs) and mobile genetic elements can further complicate effects to combat antibiotic resistance in th...

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Autores principales:	M. Ishrat Jahan, Md. Mizanur Rahaman, M. Anwar Hossain, Munawar Sultana
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Carbapenem resistance Acinetobacter baumannii β-Lactamase Aminoglycoside-modifying enzyme Class 1 integron Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/7dfaa5fa39a740f2b663c35e8c05b9ee
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spelling	oai:doaj.org-article:7dfaa5fa39a740f2b663c35e8c05b9ee2021-12-04T04:34:13ZDraft genome sequence of a carbapenem-resistant clinical Acinetobacter baumannii revealing co-existence of four classes of β-lactamases2213-716510.1016/j.jgar.2021.11.002https://doaj.org/article/7dfaa5fa39a740f2b663c35e8c05b9ee2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2213716521002526https://doaj.org/toc/2213-7165ABSTRACT: Objectives: Nosocomial carbapenem-resistant Acinetobacter baumannii is a challenge in the treatment of intensive care unit (ICU) patients. The presence of antimicrobial resistance genes (ARGs) and mobile genetic elements can further complicate effects to combat antibiotic resistance in this post-antibiotic era. The aim of this study was to analyse the molecular basis of carbapenem resistance in A. baumannii strain DMC-32a. Methods: Strain DMC-32a, isolated from a wound swab of an ICU patient, was screened phenotypically and genotypically for carbapenem resistance. The isolate was subjected whole-genome sequencing (WGS) to investigate the resistance mechanisms. Results: Strain DMC-32a was resistant to all tested antibiotics belonging to seven classes, except for the polymyxins. MICs determined against imipenem and meropenem were 512 mg/L and >512 mg/L, respectively. Gene-specific PCR confirmed the presence of blaNDM-1 and intI1. WGS confirmed the isolate as sequence type ST1053, the presence of four classes of β-lactamases [A (blaPER-7, blaTEM-116), B (blaNDM-1), C (blaADC-26) and D (blaOXA-23, blaOXA-51)], and four genes encoding aminoglycoside-modifying enzymes [aph(6)-Id, aph(3′′)-Ib, aph(3′)-VI and ant(3′′)-IIc] conferring resistance to streptomycin, amikacin, kanamycin and spectinomycin. The analysis also confirmed the presence of a class 1 integron gene cassette harbouring ARGs to rifamycin (arr-2) and phenicols (cmlA5). No plasmid replicon was found from the sequence data. Conclusion: The co-existence of four β-lactamase classes in A. baumannii DMC-32a has not been reported previously from Bangladesh and not in this ST elsewhere. The emergence of such a nosocomial pathogen creates the need for effective therapeutics for critically-ill patients and for controlling hospital-acquired infections.M. Ishrat JahanMd. Mizanur RahamanM. Anwar HossainMunawar SultanaElsevierarticleCarbapenem resistanceAcinetobacter baumanniiβ-LactamaseAminoglycoside-modifying enzymeClass 1 integronMicrobiologyQR1-502ENJournal of Global Antimicrobial Resistance, Vol 27, Iss , Pp 329-331 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Carbapenem resistance Acinetobacter baumannii β-Lactamase Aminoglycoside-modifying enzyme Class 1 integron Microbiology QR1-502
spellingShingle	Carbapenem resistance Acinetobacter baumannii β-Lactamase Aminoglycoside-modifying enzyme Class 1 integron Microbiology QR1-502 M. Ishrat Jahan Md. Mizanur Rahaman M. Anwar Hossain Munawar Sultana Draft genome sequence of a carbapenem-resistant clinical Acinetobacter baumannii revealing co-existence of four classes of β-lactamases
description	ABSTRACT: Objectives: Nosocomial carbapenem-resistant Acinetobacter baumannii is a challenge in the treatment of intensive care unit (ICU) patients. The presence of antimicrobial resistance genes (ARGs) and mobile genetic elements can further complicate effects to combat antibiotic resistance in this post-antibiotic era. The aim of this study was to analyse the molecular basis of carbapenem resistance in A. baumannii strain DMC-32a. Methods: Strain DMC-32a, isolated from a wound swab of an ICU patient, was screened phenotypically and genotypically for carbapenem resistance. The isolate was subjected whole-genome sequencing (WGS) to investigate the resistance mechanisms. Results: Strain DMC-32a was resistant to all tested antibiotics belonging to seven classes, except for the polymyxins. MICs determined against imipenem and meropenem were 512 mg/L and >512 mg/L, respectively. Gene-specific PCR confirmed the presence of blaNDM-1 and intI1. WGS confirmed the isolate as sequence type ST1053, the presence of four classes of β-lactamases [A (blaPER-7, blaTEM-116), B (blaNDM-1), C (blaADC-26) and D (blaOXA-23, blaOXA-51)], and four genes encoding aminoglycoside-modifying enzymes [aph(6)-Id, aph(3′′)-Ib, aph(3′)-VI and ant(3′′)-IIc] conferring resistance to streptomycin, amikacin, kanamycin and spectinomycin. The analysis also confirmed the presence of a class 1 integron gene cassette harbouring ARGs to rifamycin (arr-2) and phenicols (cmlA5). No plasmid replicon was found from the sequence data. Conclusion: The co-existence of four β-lactamase classes in A. baumannii DMC-32a has not been reported previously from Bangladesh and not in this ST elsewhere. The emergence of such a nosocomial pathogen creates the need for effective therapeutics for critically-ill patients and for controlling hospital-acquired infections.
format	article
author	M. Ishrat Jahan Md. Mizanur Rahaman M. Anwar Hossain Munawar Sultana
author_facet	M. Ishrat Jahan Md. Mizanur Rahaman M. Anwar Hossain Munawar Sultana
author_sort	M. Ishrat Jahan
title	Draft genome sequence of a carbapenem-resistant clinical Acinetobacter baumannii revealing co-existence of four classes of β-lactamases
title_short	Draft genome sequence of a carbapenem-resistant clinical Acinetobacter baumannii revealing co-existence of four classes of β-lactamases
title_full	Draft genome sequence of a carbapenem-resistant clinical Acinetobacter baumannii revealing co-existence of four classes of β-lactamases
title_fullStr	Draft genome sequence of a carbapenem-resistant clinical Acinetobacter baumannii revealing co-existence of four classes of β-lactamases
title_full_unstemmed	Draft genome sequence of a carbapenem-resistant clinical Acinetobacter baumannii revealing co-existence of four classes of β-lactamases
title_sort	draft genome sequence of a carbapenem-resistant clinical acinetobacter baumannii revealing co-existence of four classes of β-lactamases
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/7dfaa5fa39a740f2b663c35e8c05b9ee
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Draft genome sequence of a carbapenem-resistant clinical Acinetobacter baumannii revealing co-existence of four classes of β-lactamases

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