Determination of Levamisole and Mebendazole and Its Two Metabolite Residues in Three Poultry Species by HPLC-MS/MS

A high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed to simultaneously analyze levamisole (LMS) and mebendazole (MBZ) and its two metabolites, 5-hydroxymebendazole (HMBZ) and 2-amino-5-benzoylbenzimidazole (AMBZ), in poultry muscle (chicken, duck and go...

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Autores principales:	Pengfei Gao, Peiyang Zhang, Yawen Guo, Zhaoyuan He, Yuhao Dong, Yayun Tang, Fanxun Guan, Tao Zhang, Kaizhou Xie
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	chicken meat goose meat duck meat veterinary drugs validation method Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/7dfd15adf65141deb896b8e471115a54
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spelling	oai:doaj.org-article:7dfd15adf65141deb896b8e471115a542021-11-25T17:36:20ZDetermination of Levamisole and Mebendazole and Its Two Metabolite Residues in Three Poultry Species by HPLC-MS/MS10.3390/foods101128412304-8158https://doaj.org/article/7dfd15adf65141deb896b8e471115a542021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2841https://doaj.org/toc/2304-8158A high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed to simultaneously analyze levamisole (LMS) and mebendazole (MBZ) and its two metabolites, 5-hydroxymebendazole (HMBZ) and 2-amino-5-benzoylbenzimidazole (AMBZ), in poultry muscle (chicken, duck and goose). In the sample preparation process, basic ethyl acetate was used as the extraction agent, and the extracted samples were back-extracted with hydrochloric acid, purified by Oasis MCX solid-phase extraction (SPE) cartridges, and reconstituted in the initial mobile phase after being blown dry with nitrogen. Chromatographic separation was performed on an Xbridge C<sub>18</sub> column (4.6 mm × 150 mm, 5 μm) with 0.1% formic acid in water and acetonitrile as the mobile phases, and gradient elution was performed at a flow rate of 0.6 mL/min and a column temperature of 35 °C. In blank poultry muscle samples, the spiked concentrations of LMS, MBZ, HMBZ, and AMBZ were within the range of the limit of quantitation (LOQ) to 25 μg/kg. The peak areas of the four target drugs had a good linear relationship with the concentration, and the determination coefficient (R<sup>2</sup>) values were higher than 0.9990. The average recoveries of LMS, MBZ, HMBZ, and AMBZ were 86.77–96.94%; the intraday relative standard deviations (RSDs) were 1.75–4.99% at LOQ, 0.5 maximum residue limit (MRL), 1.0 MRL, and 2.0 MRL; the interday RSDs were 2.54–5.52%; and the LODs and LOQs were 0.04–0.30 μg/kg and 0.12–0.80 μg/kg, respectively.Pengfei GaoPeiyang ZhangYawen GuoZhaoyuan HeYuhao DongYayun TangFanxun GuanTao ZhangKaizhou XieMDPI AGarticlechicken meatgoose meatduck meatveterinary drugsvalidation methodChemical technologyTP1-1185ENFoods, Vol 10, Iss 2841, p 2841 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chicken meat goose meat duck meat veterinary drugs validation method Chemical technology TP1-1185
spellingShingle	chicken meat goose meat duck meat veterinary drugs validation method Chemical technology TP1-1185 Pengfei Gao Peiyang Zhang Yawen Guo Zhaoyuan He Yuhao Dong Yayun Tang Fanxun Guan Tao Zhang Kaizhou Xie Determination of Levamisole and Mebendazole and Its Two Metabolite Residues in Three Poultry Species by HPLC-MS/MS
description	A high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed to simultaneously analyze levamisole (LMS) and mebendazole (MBZ) and its two metabolites, 5-hydroxymebendazole (HMBZ) and 2-amino-5-benzoylbenzimidazole (AMBZ), in poultry muscle (chicken, duck and goose). In the sample preparation process, basic ethyl acetate was used as the extraction agent, and the extracted samples were back-extracted with hydrochloric acid, purified by Oasis MCX solid-phase extraction (SPE) cartridges, and reconstituted in the initial mobile phase after being blown dry with nitrogen. Chromatographic separation was performed on an Xbridge C<sub>18</sub> column (4.6 mm × 150 mm, 5 μm) with 0.1% formic acid in water and acetonitrile as the mobile phases, and gradient elution was performed at a flow rate of 0.6 mL/min and a column temperature of 35 °C. In blank poultry muscle samples, the spiked concentrations of LMS, MBZ, HMBZ, and AMBZ were within the range of the limit of quantitation (LOQ) to 25 μg/kg. The peak areas of the four target drugs had a good linear relationship with the concentration, and the determination coefficient (R<sup>2</sup>) values were higher than 0.9990. The average recoveries of LMS, MBZ, HMBZ, and AMBZ were 86.77–96.94%; the intraday relative standard deviations (RSDs) were 1.75–4.99% at LOQ, 0.5 maximum residue limit (MRL), 1.0 MRL, and 2.0 MRL; the interday RSDs were 2.54–5.52%; and the LODs and LOQs were 0.04–0.30 μg/kg and 0.12–0.80 μg/kg, respectively.
format	article
author	Pengfei Gao Peiyang Zhang Yawen Guo Zhaoyuan He Yuhao Dong Yayun Tang Fanxun Guan Tao Zhang Kaizhou Xie
author_facet	Pengfei Gao Peiyang Zhang Yawen Guo Zhaoyuan He Yuhao Dong Yayun Tang Fanxun Guan Tao Zhang Kaizhou Xie
author_sort	Pengfei Gao
title	Determination of Levamisole and Mebendazole and Its Two Metabolite Residues in Three Poultry Species by HPLC-MS/MS
title_short	Determination of Levamisole and Mebendazole and Its Two Metabolite Residues in Three Poultry Species by HPLC-MS/MS
title_full	Determination of Levamisole and Mebendazole and Its Two Metabolite Residues in Three Poultry Species by HPLC-MS/MS
title_fullStr	Determination of Levamisole and Mebendazole and Its Two Metabolite Residues in Three Poultry Species by HPLC-MS/MS
title_full_unstemmed	Determination of Levamisole and Mebendazole and Its Two Metabolite Residues in Three Poultry Species by HPLC-MS/MS
title_sort	determination of levamisole and mebendazole and its two metabolite residues in three poultry species by hplc-ms/ms
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7dfd15adf65141deb896b8e471115a54
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Determination of Levamisole and Mebendazole and Its Two Metabolite Residues in Three Poultry Species by HPLC-MS/MS

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