Mining of Cyanobacterial Genomes Indicates Natural Product Biosynthetic Gene Clusters Located in Conjugative Plasmids

Microbial natural products are compounds with unique chemical structures and diverse biological activities. Cyanobacteria commonly possess a wide range of biosynthetic gene clusters (BGCs) to produce natural products. Although natural product BGCs have been found in almost all cyanobacterial genomes...

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Autores principales:	Rafael Vicentini Popin, Danillo Oliveira Alvarenga, Raquel Castelo-Branco, David Peter Fewer, Kaarina Sivonen
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	cyanobacteria conjugation genome mining toxins geosmin Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/7dffcfeac35b41da8ec39db4a432af39
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spelling	oai:doaj.org-article:7dffcfeac35b41da8ec39db4a432af392021-11-04T09:23:54ZMining of Cyanobacterial Genomes Indicates Natural Product Biosynthetic Gene Clusters Located in Conjugative Plasmids1664-302X10.3389/fmicb.2021.684565https://doaj.org/article/7dffcfeac35b41da8ec39db4a432af392021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.684565/fullhttps://doaj.org/toc/1664-302XMicrobial natural products are compounds with unique chemical structures and diverse biological activities. Cyanobacteria commonly possess a wide range of biosynthetic gene clusters (BGCs) to produce natural products. Although natural product BGCs have been found in almost all cyanobacterial genomes, little attention has been given in cyanobacterial research to the partitioning of these biosynthetic pathways in chromosomes and plasmids. Cyanobacterial plasmids are believed to disperse several natural product BGCs, such as toxins, by plasmids through horizontal gene transfer. Therefore, plasmids may confer the ability to produce toxins and may play a role in the evolution of diverse natural product BGCs from cyanobacteria. Here, we performed an analysis of the distribution of natural product BGCs in 185 genomes and mapped the presence of genes involved in the conjugation in plasmids. The 185 analyzed genomes revealed 1817 natural products BGCs. Individual genomes contained 1–42 biosynthetic pathways (mean 8), 95% of which were present in chromosomes and the remaining 5% in plasmids. Of the 424 analyzed cyanobacterial plasmids, 12% contained homologs of genes involved in conjugation and natural product biosynthetic pathways. Among the biosynthetic pathways in plasmids, manual curation identified those to produce aeruginosin, anabaenopeptin, ambiguine, cryptophycin, hassallidin, geosmin, and microcystin. These compounds are known toxins, protease inhibitors, odorous compounds, antimicrobials, and antitumorals. The present study provides in silico evidence using genome mining that plasmids may be involved in the distribution of natural product BGCs in cyanobacteria. Consequently, cyanobacterial plasmids have importance in the context of biotechnology, water management, and public health risk assessment. Future research should explore in vivo conjugation and the end products of natural product BGCs in plasmids via chemical analyses.Rafael Vicentini PopinDanillo Oliveira AlvarengaDanillo Oliveira AlvarengaRaquel Castelo-BrancoRaquel Castelo-BrancoDavid Peter FewerKaarina SivonenFrontiers Media S.A.articlecyanobacteriaconjugationgenome miningtoxinsgeosminMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cyanobacteria conjugation genome mining toxins geosmin Microbiology QR1-502
spellingShingle	cyanobacteria conjugation genome mining toxins geosmin Microbiology QR1-502 Rafael Vicentini Popin Danillo Oliveira Alvarenga Danillo Oliveira Alvarenga Raquel Castelo-Branco Raquel Castelo-Branco David Peter Fewer Kaarina Sivonen Mining of Cyanobacterial Genomes Indicates Natural Product Biosynthetic Gene Clusters Located in Conjugative Plasmids
description	Microbial natural products are compounds with unique chemical structures and diverse biological activities. Cyanobacteria commonly possess a wide range of biosynthetic gene clusters (BGCs) to produce natural products. Although natural product BGCs have been found in almost all cyanobacterial genomes, little attention has been given in cyanobacterial research to the partitioning of these biosynthetic pathways in chromosomes and plasmids. Cyanobacterial plasmids are believed to disperse several natural product BGCs, such as toxins, by plasmids through horizontal gene transfer. Therefore, plasmids may confer the ability to produce toxins and may play a role in the evolution of diverse natural product BGCs from cyanobacteria. Here, we performed an analysis of the distribution of natural product BGCs in 185 genomes and mapped the presence of genes involved in the conjugation in plasmids. The 185 analyzed genomes revealed 1817 natural products BGCs. Individual genomes contained 1–42 biosynthetic pathways (mean 8), 95% of which were present in chromosomes and the remaining 5% in plasmids. Of the 424 analyzed cyanobacterial plasmids, 12% contained homologs of genes involved in conjugation and natural product biosynthetic pathways. Among the biosynthetic pathways in plasmids, manual curation identified those to produce aeruginosin, anabaenopeptin, ambiguine, cryptophycin, hassallidin, geosmin, and microcystin. These compounds are known toxins, protease inhibitors, odorous compounds, antimicrobials, and antitumorals. The present study provides in silico evidence using genome mining that plasmids may be involved in the distribution of natural product BGCs in cyanobacteria. Consequently, cyanobacterial plasmids have importance in the context of biotechnology, water management, and public health risk assessment. Future research should explore in vivo conjugation and the end products of natural product BGCs in plasmids via chemical analyses.
format	article
author	Rafael Vicentini Popin Danillo Oliveira Alvarenga Danillo Oliveira Alvarenga Raquel Castelo-Branco Raquel Castelo-Branco David Peter Fewer Kaarina Sivonen
author_facet	Rafael Vicentini Popin Danillo Oliveira Alvarenga Danillo Oliveira Alvarenga Raquel Castelo-Branco Raquel Castelo-Branco David Peter Fewer Kaarina Sivonen
author_sort	Rafael Vicentini Popin
title	Mining of Cyanobacterial Genomes Indicates Natural Product Biosynthetic Gene Clusters Located in Conjugative Plasmids
title_short	Mining of Cyanobacterial Genomes Indicates Natural Product Biosynthetic Gene Clusters Located in Conjugative Plasmids
title_full	Mining of Cyanobacterial Genomes Indicates Natural Product Biosynthetic Gene Clusters Located in Conjugative Plasmids
title_fullStr	Mining of Cyanobacterial Genomes Indicates Natural Product Biosynthetic Gene Clusters Located in Conjugative Plasmids
title_full_unstemmed	Mining of Cyanobacterial Genomes Indicates Natural Product Biosynthetic Gene Clusters Located in Conjugative Plasmids
title_sort	mining of cyanobacterial genomes indicates natural product biosynthetic gene clusters located in conjugative plasmids
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/7dffcfeac35b41da8ec39db4a432af39
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_version_	1718444972062015488

Mining of Cyanobacterial Genomes Indicates Natural Product Biosynthetic Gene Clusters Located in Conjugative Plasmids

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