Association of FoxP3/Scurfin Germline Polymorphism (C-2383T/rs3761549) with Colorectal Cancer

Background FoxP3 gene encodes a transcription factor with crucial roles in the development and function of regulatory T cells. Objectives The present study was conducted to investigate whether the C-2383T (rs3761549) polymorphism in the promoter region of the FoxP3 gene is associated with color...

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Autores principales:	Zahra Mojtahedi, Nasrollah Erfani, Mohammad Reza Haghshenas, Seyed Vahid Hosseini, Abbas Ghaderi
Formato:	article
Lenguaje:	EN
Publicado:	Shiraz University of Medical Sciences 2013
Materias:	colorectal cancer foxp3 polymorphism single nucleotide polymorphisms (snp) Medicine R
Acceso en línea:	https://doaj.org/article/7dffdc863c6d45b48f9077de25a39de8
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spelling	oai:doaj.org-article:7dffdc863c6d45b48f9077de25a39de82021-11-14T06:43:17ZAssociation of FoxP3/Scurfin Germline Polymorphism (C-2383T/rs3761549) with Colorectal Cancer2783-243010.17795/acr-11478https://doaj.org/article/7dffdc863c6d45b48f9077de25a39de82013-06-01T00:00:00Zhttps://colorectalresearch.sums.ac.ir/article_45440_0c540f5ca9ff4c46f836c1e5466ac97e.pdfhttps://doaj.org/toc/2783-2430Background FoxP3 gene encodes a transcription factor with crucial roles in the development and function of regulatory T cells. Objectives The present study was conducted to investigate whether the C-2383T (rs3761549) polymorphism in the promoter region of the FoxP3 gene is associated with colorectal cancer. Patients and Methods The study groups consist of 108 patients (52 men and 56 women) with colorectal cancer and 187 (126 men and 61 women) healthy individuals. They were genotyped for the FoxP3 polymorphism at position -2383 C > T using polymerase chain reaction-restriction fragment length polymorphism method. Frequencies of the gene variants were analyzed separately in men and women since FoxP3 is an X-linked gene. Results The numbers of female patients with C/C, C/T and T/T genotypes were 48 (85.7%), 7 (12.5%) and 1 (1.8%), while in female controls, they were 53 (86.9%), 8 (13.1%) and 0 (0%), respectively. In male patients and controls, frequencies of the C genotype were 49 (94.2%) and 118 (93.7%), and the T genotype were 3 (5.8%) and 8 (6.3%), respectively. Genotype frequencies were not significantly different between controls and colorectal cancer patients. Distance metastasis was significantly associated with the FoxP3 polymorphism as calculated using Pearson’s chi-squared test (P = 0.006 in men and P = 0.03 in women). While 50% of metastatic patients had T or C/T (or T/T) genotype, the percentage of this genotype in non-metastatic patients was 4% in men and 11.5% in women. However, P values did not remain significant if differences were calculated using two-sided Fisher׳s exact test (P = 0.11 in men, and P = 0.09 in women); this might be due to the low number of our metastatic patients. Other characteristics of patients including age, tumor grade and stage were not significantly associated with the polymorphism. Conclusions Our results supported an association between C-2383T FoxP3 polymorphism and metastatic colorectal cancer in southern Iran.Zahra MojtahediNasrollah ErfaniMohammad Reza HaghshenasSeyed Vahid HosseiniAbbas GhaderiShiraz University of Medical Sciencesarticlecolorectal cancerfoxp3polymorphismsingle nucleotide polymorphisms (snp)MedicineRENIranian Journal of Colorectal Research, Vol 1, Iss 1, Pp 1-6 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	colorectal cancer foxp3 polymorphism single nucleotide polymorphisms (snp) Medicine R
spellingShingle	colorectal cancer foxp3 polymorphism single nucleotide polymorphisms (snp) Medicine R Zahra Mojtahedi Nasrollah Erfani Mohammad Reza Haghshenas Seyed Vahid Hosseini Abbas Ghaderi Association of FoxP3/Scurfin Germline Polymorphism (C-2383T/rs3761549) with Colorectal Cancer
description	Background FoxP3 gene encodes a transcription factor with crucial roles in the development and function of regulatory T cells. Objectives The present study was conducted to investigate whether the C-2383T (rs3761549) polymorphism in the promoter region of the FoxP3 gene is associated with colorectal cancer. Patients and Methods The study groups consist of 108 patients (52 men and 56 women) with colorectal cancer and 187 (126 men and 61 women) healthy individuals. They were genotyped for the FoxP3 polymorphism at position -2383 C > T using polymerase chain reaction-restriction fragment length polymorphism method. Frequencies of the gene variants were analyzed separately in men and women since FoxP3 is an X-linked gene. Results The numbers of female patients with C/C, C/T and T/T genotypes were 48 (85.7%), 7 (12.5%) and 1 (1.8%), while in female controls, they were 53 (86.9%), 8 (13.1%) and 0 (0%), respectively. In male patients and controls, frequencies of the C genotype were 49 (94.2%) and 118 (93.7%), and the T genotype were 3 (5.8%) and 8 (6.3%), respectively. Genotype frequencies were not significantly different between controls and colorectal cancer patients. Distance metastasis was significantly associated with the FoxP3 polymorphism as calculated using Pearson’s chi-squared test (P = 0.006 in men and P = 0.03 in women). While 50% of metastatic patients had T or C/T (or T/T) genotype, the percentage of this genotype in non-metastatic patients was 4% in men and 11.5% in women. However, P values did not remain significant if differences were calculated using two-sided Fisher׳s exact test (P = 0.11 in men, and P = 0.09 in women); this might be due to the low number of our metastatic patients. Other characteristics of patients including age, tumor grade and stage were not significantly associated with the polymorphism. Conclusions Our results supported an association between C-2383T FoxP3 polymorphism and metastatic colorectal cancer in southern Iran.
format	article
author	Zahra Mojtahedi Nasrollah Erfani Mohammad Reza Haghshenas Seyed Vahid Hosseini Abbas Ghaderi
author_facet	Zahra Mojtahedi Nasrollah Erfani Mohammad Reza Haghshenas Seyed Vahid Hosseini Abbas Ghaderi
author_sort	Zahra Mojtahedi
title	Association of FoxP3/Scurfin Germline Polymorphism (C-2383T/rs3761549) with Colorectal Cancer
title_short	Association of FoxP3/Scurfin Germline Polymorphism (C-2383T/rs3761549) with Colorectal Cancer
title_full	Association of FoxP3/Scurfin Germline Polymorphism (C-2383T/rs3761549) with Colorectal Cancer
title_fullStr	Association of FoxP3/Scurfin Germline Polymorphism (C-2383T/rs3761549) with Colorectal Cancer
title_full_unstemmed	Association of FoxP3/Scurfin Germline Polymorphism (C-2383T/rs3761549) with Colorectal Cancer
title_sort	association of foxp3/scurfin germline polymorphism (c-2383t/rs3761549) with colorectal cancer
publisher	Shiraz University of Medical Sciences
publishDate	2013
url	https://doaj.org/article/7dffdc863c6d45b48f9077de25a39de8
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Association of FoxP3/Scurfin Germline Polymorphism (C-2383T/rs3761549) with Colorectal Cancer

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