Identification and validation of circulating miRNAs as potential new biomarkers for severe liver disease in patients with leptospirosis.

<h4>Background</h4>Leptospirosis, a global zoonotic infectious disease, has various clinical manifestations ranging from mild self-limiting illness to life-threatening with multi-organ damage, including liver involvement. This study was aimed at identifying circulating microRNAs (miRNAs)...

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Autores principales:	Natthaya Chuaypen, Umaporn Limothai, Pattapon Kunadirek, Pornchai Kaewsapsak, Patipark Kueanjinda, Nattachai Srisawat, Pisit Tangkijvanich
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7e049dabeca842cc9af9b52b75e19de4
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spelling	oai:doaj.org-article:7e049dabeca842cc9af9b52b75e19de42021-12-02T20:14:08ZIdentification and validation of circulating miRNAs as potential new biomarkers for severe liver disease in patients with leptospirosis.1932-620310.1371/journal.pone.0257805https://doaj.org/article/7e049dabeca842cc9af9b52b75e19de42021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257805https://doaj.org/toc/1932-6203<h4>Background</h4>Leptospirosis, a global zoonotic infectious disease, has various clinical manifestations ranging from mild self-limiting illness to life-threatening with multi-organ damage, including liver involvement. This study was aimed at identifying circulating microRNAs (miRNAs) as novel biomarkers for predicting severe liver involvement in patients with leptospirosis.<h4>Methods</h4>In a discovery set, 12 serum samples of patients with anicteric and icteric leptospirosis at initial clinical presentation were used for miRNA profiling by a NanoString nCounter miRNA assay. In a validated cohort, top candidate miRNAs were selected and further tested by qRT-PCR in serum samples of 81 and 16 individuals with anicteric and icteric leptospirosis, respectively.<h4>Results</h4>The discovery set identified 38 significantly differential expression miRNAs between the two groups. Among these, miR-601 and miR-630 were selected as the top two candidates significantly up-regulated expressed in the icteric group. The enriched KEGG pathway showed that these miRNAs were mainly involved in immune responses and inflammation. In the validated cohort, miR-601 and miR-630 levels were significantly higher in the icteric group compared with the anicteric group. Additionally, these two miRNAs displayed good predictors of subsequent acute liver failure with a high sensitivity of 100%. On regression analysis, elevated miR-601 and miR-630 expression were also predictive of multi-organ failures and poor overall survival.<h4>Conclusion</h4>Our data indicated that miRNA expression profiles were significantly differentiated between the icteric and anicteric groups. Serum miR-601 and miR-630 at presentation could potentially serve as promising biomarkers for predicting subsequent acute liver failure and overall survival in patients with leptospirosis.Natthaya ChuaypenUmaporn LimothaiPattapon KunadirekPornchai KaewsapsakPatipark KueanjindaNattachai SrisawatPisit TangkijvanichPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0257805 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Natthaya Chuaypen Umaporn Limothai Pattapon Kunadirek Pornchai Kaewsapsak Patipark Kueanjinda Nattachai Srisawat Pisit Tangkijvanich Identification and validation of circulating miRNAs as potential new biomarkers for severe liver disease in patients with leptospirosis.
description	<h4>Background</h4>Leptospirosis, a global zoonotic infectious disease, has various clinical manifestations ranging from mild self-limiting illness to life-threatening with multi-organ damage, including liver involvement. This study was aimed at identifying circulating microRNAs (miRNAs) as novel biomarkers for predicting severe liver involvement in patients with leptospirosis.<h4>Methods</h4>In a discovery set, 12 serum samples of patients with anicteric and icteric leptospirosis at initial clinical presentation were used for miRNA profiling by a NanoString nCounter miRNA assay. In a validated cohort, top candidate miRNAs were selected and further tested by qRT-PCR in serum samples of 81 and 16 individuals with anicteric and icteric leptospirosis, respectively.<h4>Results</h4>The discovery set identified 38 significantly differential expression miRNAs between the two groups. Among these, miR-601 and miR-630 were selected as the top two candidates significantly up-regulated expressed in the icteric group. The enriched KEGG pathway showed that these miRNAs were mainly involved in immune responses and inflammation. In the validated cohort, miR-601 and miR-630 levels were significantly higher in the icteric group compared with the anicteric group. Additionally, these two miRNAs displayed good predictors of subsequent acute liver failure with a high sensitivity of 100%. On regression analysis, elevated miR-601 and miR-630 expression were also predictive of multi-organ failures and poor overall survival.<h4>Conclusion</h4>Our data indicated that miRNA expression profiles were significantly differentiated between the icteric and anicteric groups. Serum miR-601 and miR-630 at presentation could potentially serve as promising biomarkers for predicting subsequent acute liver failure and overall survival in patients with leptospirosis.
format	article
author	Natthaya Chuaypen Umaporn Limothai Pattapon Kunadirek Pornchai Kaewsapsak Patipark Kueanjinda Nattachai Srisawat Pisit Tangkijvanich
author_facet	Natthaya Chuaypen Umaporn Limothai Pattapon Kunadirek Pornchai Kaewsapsak Patipark Kueanjinda Nattachai Srisawat Pisit Tangkijvanich
author_sort	Natthaya Chuaypen
title	Identification and validation of circulating miRNAs as potential new biomarkers for severe liver disease in patients with leptospirosis.
title_short	Identification and validation of circulating miRNAs as potential new biomarkers for severe liver disease in patients with leptospirosis.
title_full	Identification and validation of circulating miRNAs as potential new biomarkers for severe liver disease in patients with leptospirosis.
title_fullStr	Identification and validation of circulating miRNAs as potential new biomarkers for severe liver disease in patients with leptospirosis.
title_full_unstemmed	Identification and validation of circulating miRNAs as potential new biomarkers for severe liver disease in patients with leptospirosis.
title_sort	identification and validation of circulating mirnas as potential new biomarkers for severe liver disease in patients with leptospirosis.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/7e049dabeca842cc9af9b52b75e19de4
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Identification and validation of circulating miRNAs as potential new biomarkers for severe liver disease in patients with leptospirosis.

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