Managing Contamination and Diverse Bacterial Loads in 16S rRNA Deep Sequencing of Clinical Samples: Implications of the Law of Small Numbers

There has been a gradual increase in 16S deep sequencing studies on infectious disease materials. Management of bacterial DNA contamination is a major challenge in such diagnostics, particularly in low biomass samples.

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Autores principales:	Ruben Dyrhovden, Martin Rippin, Kjell Kåre Øvrebø, Randi M. Nygaard, Elling Ulvestad, Øyvind Kommedal
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2021
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/7e158acfaad245d589d8e7bb2c45acab
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spelling	oai:doaj.org-article:7e158acfaad245d589d8e7bb2c45acab2021-11-03T21:15:03ZManaging Contamination and Diverse Bacterial Loads in 16S rRNA Deep Sequencing of Clinical Samples: Implications of the Law of Small Numbers2150-751110.1128/mBio.00598-21https://doaj.org/article/7e158acfaad245d589d8e7bb2c45acab2021-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00598-21https://doaj.org/toc/2150-7511 There has been a gradual increase in 16S deep sequencing studies on infectious disease materials. Management of bacterial DNA contamination is a major challenge in such diagnostics, particularly in low biomass samples.Ruben DyrhovdenMartin RippinKjell Kåre ØvrebøRandi M. NygaardElling UlvestadØyvind KommedalAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 12, Iss 3 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Ruben Dyrhovden Martin Rippin Kjell Kåre Øvrebø Randi M. Nygaard Elling Ulvestad Øyvind Kommedal Managing Contamination and Diverse Bacterial Loads in 16S rRNA Deep Sequencing of Clinical Samples: Implications of the Law of Small Numbers
description	There has been a gradual increase in 16S deep sequencing studies on infectious disease materials. Management of bacterial DNA contamination is a major challenge in such diagnostics, particularly in low biomass samples.
format	article
author	Ruben Dyrhovden Martin Rippin Kjell Kåre Øvrebø Randi M. Nygaard Elling Ulvestad Øyvind Kommedal
author_facet	Ruben Dyrhovden Martin Rippin Kjell Kåre Øvrebø Randi M. Nygaard Elling Ulvestad Øyvind Kommedal
author_sort	Ruben Dyrhovden
title	Managing Contamination and Diverse Bacterial Loads in 16S rRNA Deep Sequencing of Clinical Samples: Implications of the Law of Small Numbers
title_short	Managing Contamination and Diverse Bacterial Loads in 16S rRNA Deep Sequencing of Clinical Samples: Implications of the Law of Small Numbers
title_full	Managing Contamination and Diverse Bacterial Loads in 16S rRNA Deep Sequencing of Clinical Samples: Implications of the Law of Small Numbers
title_fullStr	Managing Contamination and Diverse Bacterial Loads in 16S rRNA Deep Sequencing of Clinical Samples: Implications of the Law of Small Numbers
title_full_unstemmed	Managing Contamination and Diverse Bacterial Loads in 16S rRNA Deep Sequencing of Clinical Samples: Implications of the Law of Small Numbers
title_sort	managing contamination and diverse bacterial loads in 16s rrna deep sequencing of clinical samples: implications of the law of small numbers
publisher	American Society for Microbiology
publishDate	2021
url	https://doaj.org/article/7e158acfaad245d589d8e7bb2c45acab
work_keys_str_mv	AT rubendyrhovden managingcontaminationanddiversebacterialloadsin16srrnadeepsequencingofclinicalsamplesimplicationsofthelawofsmallnumbers AT martinrippin managingcontaminationanddiversebacterialloadsin16srrnadeepsequencingofclinicalsamplesimplicationsofthelawofsmallnumbers AT kjellkareøvrebø managingcontaminationanddiversebacterialloadsin16srrnadeepsequencingofclinicalsamplesimplicationsofthelawofsmallnumbers AT randimnygaard managingcontaminationanddiversebacterialloadsin16srrnadeepsequencingofclinicalsamplesimplicationsofthelawofsmallnumbers AT ellingulvestad managingcontaminationanddiversebacterialloadsin16srrnadeepsequencingofclinicalsamplesimplicationsofthelawofsmallnumbers AT øyvindkommedal managingcontaminationanddiversebacterialloadsin16srrnadeepsequencingofclinicalsamplesimplicationsofthelawofsmallnumbers
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Managing Contamination and Diverse Bacterial Loads in 16S rRNA Deep Sequencing of Clinical Samples: Implications of the Law of Small Numbers

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