Delivery of baicalein and paclitaxel using self-assembled nanoparticles: synergistic antitumor effect in vitro and in vivo

Wei Wang,1,* Mei Xi,2,* Xuezhong Duan,1 Yong Wang,3 Fansheng Kong4 1Department of Chinese Medicine Integrated Traditional Chinese Medicine and Western Medicine, General Hospital of Ji’nan Command, People’s Liberation Army, 2Emergency Department, The Fourth People’s Hos...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wang W, Xi M, Duan X, Wang Y, Kong F
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7e1c3d72eecf429fba1994e20bc6f075
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7e1c3d72eecf429fba1994e20bc6f075
record_format	dspace
spelling	oai:doaj.org-article:7e1c3d72eecf429fba1994e20bc6f0752021-12-02T01:49:52ZDelivery of baicalein and paclitaxel using self-assembled nanoparticles: synergistic antitumor effect in vitro and in vivo1178-2013https://doaj.org/article/7e1c3d72eecf429fba1994e20bc6f0752015-05-01T00:00:00Zhttp://www.dovepress.com/delivery-of-baicalein-and-paclitaxel-using-self-assembled-nanoparticle-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Wei Wang,1,* Mei Xi,2,* Xuezhong Duan,1 Yong Wang,3 Fansheng Kong4 1Department of Chinese Medicine Integrated Traditional Chinese Medicine and Western Medicine, General Hospital of Ji’nan Command, People’s Liberation Army, 2Emergency Department, The Fourth People’s Hospital of Ji’nan, Medical School, Tai Shan Medical College, 3Department of Rehabilitation and Physiotherapy, General Hospital of Ji’nan Command, People’s Liberation Army, 4Department of Hematology, General Hospital of Ji’nan Command, People’s Liberation Army, Ji’nan, People’s Republic of China *These authors contributed equally to this work Purpose: Combination anticancer therapy is promising to generate synergistic anticancer effects to maximize the treatment effect and overcome multidrug resistance. The aim of the study reported here was to develop multifunctional, dual-ligand, modified, self-assembled nanoparticles (NPs) for the combination delivery of baicalein (BCL) and paclitaxel (PTX) prodrugs.Methods: Prodrug of PTX and prodrug of BCL, containing dual-targeted ligands of folate (FA) and hyaluronic acid (HA), were synthesized. Multifunctional self-assembled NPs for combination delivery of PTX prodrug and BCL prodrug (PTX-BCL) were prepared and the synergistic antitumor effect was evaluated in vitro and in vivo. The in vitro transfection efficiency of the novel modified vectors was evaluated in human lung cancer A549 cells and drug-resistant lung cancer A549/PTX cells. The in vivo antitumor efficiency and systemic toxicity of different formulations were further investigated in mice bearing A549/PTX drug-resistant human lung cancer xenografts.Results: The size of the PTX-BCL NPs was approximately 90 nm, with a positive zeta potential of +3.3. The PTX-BCL NPs displayed remarkably better antitumor activity over a wide range of drug concentrations, and showed an obvious synergism effect with CI50 values of 0.707 and 0.513, indicating that double-ligand modification and the co-delivery of PTX and BCL prodrugs with self-assembled NPs had remarkable superiority over other formulations.Conclusion: The prepared PTX-BCL NP drug-delivery system was proven efficient by its targeting of drug-resistant human lung cancer cells and delivering of BCL and PTX prodrugs. Enhanced synergistic anticancer effects were achieved by PTX-BCL NPs, and multidrug resistance of PTX was overcome by this promising targeted nanomedicine. Keywords: combination chemotherapy, prodrug-based nano-drug delivery system, multidrug resistance, self-assembled nanoparticlesWang WXi MDuan XWang YKong FDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 3737-3750 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Wang W Xi M Duan X Wang Y Kong F Delivery of baicalein and paclitaxel using self-assembled nanoparticles: synergistic antitumor effect in vitro and in vivo
description	Wei Wang,1,* Mei Xi,2,* Xuezhong Duan,1 Yong Wang,3 Fansheng Kong4 1Department of Chinese Medicine Integrated Traditional Chinese Medicine and Western Medicine, General Hospital of Ji’nan Command, People’s Liberation Army, 2Emergency Department, The Fourth People’s Hospital of Ji’nan, Medical School, Tai Shan Medical College, 3Department of Rehabilitation and Physiotherapy, General Hospital of Ji’nan Command, People’s Liberation Army, 4Department of Hematology, General Hospital of Ji’nan Command, People’s Liberation Army, Ji’nan, People’s Republic of China *These authors contributed equally to this work Purpose: Combination anticancer therapy is promising to generate synergistic anticancer effects to maximize the treatment effect and overcome multidrug resistance. The aim of the study reported here was to develop multifunctional, dual-ligand, modified, self-assembled nanoparticles (NPs) for the combination delivery of baicalein (BCL) and paclitaxel (PTX) prodrugs.Methods: Prodrug of PTX and prodrug of BCL, containing dual-targeted ligands of folate (FA) and hyaluronic acid (HA), were synthesized. Multifunctional self-assembled NPs for combination delivery of PTX prodrug and BCL prodrug (PTX-BCL) were prepared and the synergistic antitumor effect was evaluated in vitro and in vivo. The in vitro transfection efficiency of the novel modified vectors was evaluated in human lung cancer A549 cells and drug-resistant lung cancer A549/PTX cells. The in vivo antitumor efficiency and systemic toxicity of different formulations were further investigated in mice bearing A549/PTX drug-resistant human lung cancer xenografts.Results: The size of the PTX-BCL NPs was approximately 90 nm, with a positive zeta potential of +3.3. The PTX-BCL NPs displayed remarkably better antitumor activity over a wide range of drug concentrations, and showed an obvious synergism effect with CI50 values of 0.707 and 0.513, indicating that double-ligand modification and the co-delivery of PTX and BCL prodrugs with self-assembled NPs had remarkable superiority over other formulations.Conclusion: The prepared PTX-BCL NP drug-delivery system was proven efficient by its targeting of drug-resistant human lung cancer cells and delivering of BCL and PTX prodrugs. Enhanced synergistic anticancer effects were achieved by PTX-BCL NPs, and multidrug resistance of PTX was overcome by this promising targeted nanomedicine. Keywords: combination chemotherapy, prodrug-based nano-drug delivery system, multidrug resistance, self-assembled nanoparticles
format	article
author	Wang W Xi M Duan X Wang Y Kong F
author_facet	Wang W Xi M Duan X Wang Y Kong F
author_sort	Wang W
title	Delivery of baicalein and paclitaxel using self-assembled nanoparticles: synergistic antitumor effect in vitro and in vivo
title_short	Delivery of baicalein and paclitaxel using self-assembled nanoparticles: synergistic antitumor effect in vitro and in vivo
title_full	Delivery of baicalein and paclitaxel using self-assembled nanoparticles: synergistic antitumor effect in vitro and in vivo
title_fullStr	Delivery of baicalein and paclitaxel using self-assembled nanoparticles: synergistic antitumor effect in vitro and in vivo
title_full_unstemmed	Delivery of baicalein and paclitaxel using self-assembled nanoparticles: synergistic antitumor effect in vitro and in vivo
title_sort	delivery of baicalein and paclitaxel using self-assembled nanoparticles: synergistic antitumor effect in vitro and in vivo
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/7e1c3d72eecf429fba1994e20bc6f075
work_keys_str_mv	AT wangw deliveryofbaicaleinandpaclitaxelusingselfassemblednanoparticlessynergisticantitumoreffectinvitroandinvivo AT xim deliveryofbaicaleinandpaclitaxelusingselfassemblednanoparticlessynergisticantitumoreffectinvitroandinvivo AT duanx deliveryofbaicaleinandpaclitaxelusingselfassemblednanoparticlessynergisticantitumoreffectinvitroandinvivo AT wangy deliveryofbaicaleinandpaclitaxelusingselfassemblednanoparticlessynergisticantitumoreffectinvitroandinvivo AT kongf deliveryofbaicaleinandpaclitaxelusingselfassemblednanoparticlessynergisticantitumoreffectinvitroandinvivo
_version_	1718402849326497792

Delivery of baicalein and paclitaxel using self-assembled nanoparticles: synergistic antitumor effect in vitro and in vivo

Ejemplares similares