Effects of Temperature on the Meiotic Recombination Landscape of the Yeast <italic toggle="yes">Saccharomyces cerevisiae</italic>

ABSTRACT Although meiosis in warm-blooded organisms takes place in a narrow temperature range, meiosis in many organisms occurs over a wide variety of temperatures. We analyzed the properties of meiosis in the yeast Saccharomyces cerevisiae in cells sporulated at 14°C, 30°C, or 37°C. Using comparati...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ke Zhang, Xue-Chang Wu, Dao-Qiong Zheng, Thomas D. Petes
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	hot spot meiotic recombination microarray temperature yeast Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/7e1c619cb72f465ba061962abf471fc9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7e1c619cb72f465ba061962abf471fc9
record_format	dspace
spelling	oai:doaj.org-article:7e1c619cb72f465ba061962abf471fc92021-11-15T15:51:55ZEffects of Temperature on the Meiotic Recombination Landscape of the Yeast <italic toggle="yes">Saccharomyces cerevisiae</italic>10.1128/mBio.02099-172150-7511https://doaj.org/article/7e1c619cb72f465ba061962abf471fc92017-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02099-17https://doaj.org/toc/2150-7511ABSTRACT Although meiosis in warm-blooded organisms takes place in a narrow temperature range, meiosis in many organisms occurs over a wide variety of temperatures. We analyzed the properties of meiosis in the yeast Saccharomyces cerevisiae in cells sporulated at 14°C, 30°C, or 37°C. Using comparative-genomic-hybridization microarrays, we examined the distribution of Spo11-generated meiosis-specific double-stranded DNA breaks throughout the genome. Although there were between 300 and 400 regions of the genome with high levels of recombination (hot spots) observed at each temperature, only about 20% of these hot spots were found to have occurred independently of the temperature. In S. cerevisiae, regions near the telomeres and centromeres tend to have low levels of meiotic recombination. This tendency was observed in cells sporulated at 14°C and 30°C, but not at 37°C. Thus, the temperature of sporulation in yeast affects some global property of chromosome structure relevant to meiotic recombination. Using single-nucleotide polymorphism (SNP)-specific whole-genome microarrays, we also examined crossovers and their associated gene conversion events as well as gene conversion events that were unassociated with crossovers in all four spores of tetrads obtained by sporulation of diploids at 14°C, 30°C, or 37°C. Although tetrads from cells sporulated at 30°C had slightly (20%) more crossovers than those derived from cells sporulated at the other two temperatures, spore viability was good at all three temperatures. Thus, despite temperature-induced variation in the genetic maps, yeast cells produce viable haploid products at a wide variety of sporulation temperatures. IMPORTANCE In the yeast Saccharomyces cerevisiae, recombination is usually studied in cells that undergo meiosis at 25°C or 30°C. In a genome-wide analysis, we showed that the locations of genomic regions with high and low levels of meiotic recombination (hot spots and cold spots, respectively) differed dramatically in cells sporulated at 14°C, 30°C, and 37°C. Thus, in yeast, and likely in other non-warm-blooded organisms, genetic maps are strongly affected by the environment.Ke ZhangXue-Chang WuDao-Qiong ZhengThomas D. PetesAmerican Society for Microbiologyarticlehot spotmeiotic recombinationmicroarraytemperatureyeastMicrobiologyQR1-502ENmBio, Vol 8, Iss 6 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	hot spot meiotic recombination microarray temperature yeast Microbiology QR1-502
spellingShingle	hot spot meiotic recombination microarray temperature yeast Microbiology QR1-502 Ke Zhang Xue-Chang Wu Dao-Qiong Zheng Thomas D. Petes Effects of Temperature on the Meiotic Recombination Landscape of the Yeast <italic toggle="yes">Saccharomyces cerevisiae</italic>
description	ABSTRACT Although meiosis in warm-blooded organisms takes place in a narrow temperature range, meiosis in many organisms occurs over a wide variety of temperatures. We analyzed the properties of meiosis in the yeast Saccharomyces cerevisiae in cells sporulated at 14°C, 30°C, or 37°C. Using comparative-genomic-hybridization microarrays, we examined the distribution of Spo11-generated meiosis-specific double-stranded DNA breaks throughout the genome. Although there were between 300 and 400 regions of the genome with high levels of recombination (hot spots) observed at each temperature, only about 20% of these hot spots were found to have occurred independently of the temperature. In S. cerevisiae, regions near the telomeres and centromeres tend to have low levels of meiotic recombination. This tendency was observed in cells sporulated at 14°C and 30°C, but not at 37°C. Thus, the temperature of sporulation in yeast affects some global property of chromosome structure relevant to meiotic recombination. Using single-nucleotide polymorphism (SNP)-specific whole-genome microarrays, we also examined crossovers and their associated gene conversion events as well as gene conversion events that were unassociated with crossovers in all four spores of tetrads obtained by sporulation of diploids at 14°C, 30°C, or 37°C. Although tetrads from cells sporulated at 30°C had slightly (20%) more crossovers than those derived from cells sporulated at the other two temperatures, spore viability was good at all three temperatures. Thus, despite temperature-induced variation in the genetic maps, yeast cells produce viable haploid products at a wide variety of sporulation temperatures. IMPORTANCE In the yeast Saccharomyces cerevisiae, recombination is usually studied in cells that undergo meiosis at 25°C or 30°C. In a genome-wide analysis, we showed that the locations of genomic regions with high and low levels of meiotic recombination (hot spots and cold spots, respectively) differed dramatically in cells sporulated at 14°C, 30°C, and 37°C. Thus, in yeast, and likely in other non-warm-blooded organisms, genetic maps are strongly affected by the environment.
format	article
author	Ke Zhang Xue-Chang Wu Dao-Qiong Zheng Thomas D. Petes
author_facet	Ke Zhang Xue-Chang Wu Dao-Qiong Zheng Thomas D. Petes
author_sort	Ke Zhang
title	Effects of Temperature on the Meiotic Recombination Landscape of the Yeast <italic toggle="yes">Saccharomyces cerevisiae</italic>
title_short	Effects of Temperature on the Meiotic Recombination Landscape of the Yeast <italic toggle="yes">Saccharomyces cerevisiae</italic>
title_full	Effects of Temperature on the Meiotic Recombination Landscape of the Yeast <italic toggle="yes">Saccharomyces cerevisiae</italic>
title_fullStr	Effects of Temperature on the Meiotic Recombination Landscape of the Yeast <italic toggle="yes">Saccharomyces cerevisiae</italic>
title_full_unstemmed	Effects of Temperature on the Meiotic Recombination Landscape of the Yeast <italic toggle="yes">Saccharomyces cerevisiae</italic>
title_sort	effects of temperature on the meiotic recombination landscape of the yeast <italic toggle="yes">saccharomyces cerevisiae</italic>
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/7e1c619cb72f465ba061962abf471fc9
work_keys_str_mv	AT kezhang effectsoftemperatureonthemeioticrecombinationlandscapeoftheyeastitalictoggleyessaccharomycescerevisiaeitalic AT xuechangwu effectsoftemperatureonthemeioticrecombinationlandscapeoftheyeastitalictoggleyessaccharomycescerevisiaeitalic AT daoqiongzheng effectsoftemperatureonthemeioticrecombinationlandscapeoftheyeastitalictoggleyessaccharomycescerevisiaeitalic AT thomasdpetes effectsoftemperatureonthemeioticrecombinationlandscapeoftheyeastitalictoggleyessaccharomycescerevisiaeitalic
_version_	1718427347271548928

Effects of Temperature on the Meiotic Recombination Landscape of the Yeast <italic toggle="yes">Saccharomyces cerevisiae</italic>

Ejemplares similares