Berberine Suppresses Mice Depression Behaviors and Promotes Hippocampal Neurons Growth Through Regulating the miR-34b-5p/miR-470-5p/BDNF Axis

Yuhua Zhan, Jiyang Han, Jing Xia, Xumei Wang Department of Psychiatry, Shengjing Hospital of China Medical University, Shenyang City, Liaoning Province, People’s Republic of ChinaCorrespondence: Xumei WangDepartment of Psychiatry, Shengjing Hospital of China Medical University, Shenyang Ci...

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Autores principales:	Zhan Y, Han J, Xia J, Wang X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	depression disorder berberine mir-34b-5p mir-470-5p bdnf Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/7e1eb88fb18b42cfa71f19ea89a18281
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spelling	oai:doaj.org-article:7e1eb88fb18b42cfa71f19ea89a182812021-12-02T14:29:25ZBerberine Suppresses Mice Depression Behaviors and Promotes Hippocampal Neurons Growth Through Regulating the miR-34b-5p/miR-470-5p/BDNF Axis1178-2021https://doaj.org/article/7e1eb88fb18b42cfa71f19ea89a182812021-02-01T00:00:00Zhttps://www.dovepress.com/berberine-suppresses-mice-depression-behaviors-and-promotes-hippocampa-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Yuhua Zhan, Jiyang Han, Jing Xia, Xumei Wang Department of Psychiatry, Shengjing Hospital of China Medical University, Shenyang City, Liaoning Province, People’s Republic of ChinaCorrespondence: Xumei WangDepartment of Psychiatry, Shengjing Hospital of China Medical University, Shenyang City, Liaoning Province, People’s Republic of ChinaTel/Fax +86 0249-661566112Email xiaoyema2020@163.comBackground: Berberine has been found to inhibit the progression of depression disorder, but its specific mechanism is still unclear. MicroRNA (miRNA) is considered to play an important role in the progression of depression. However, it is unclear whether Berberine is involved in the regulation of depression progression through miRNA.Methods: The chronic unpredictable mild stress (CUMS) mice model was constructed. Mice depression behaviors were evaluated by sucrose preference test (SPT) and forced swim test (FST). Quantitative real-time PCR was employed to assess the expression of miR-34b-5p, miR-470-5p and brain-derived neurotrophic factor (BDNF). The protein expression of BDNF was examined using Western blot analysis. In addition, the viability and apoptosis of hippocampal neurons were determined using cell counting kit 8 assay, flow cytometry and TUNEL assay. The interaction between BDNF and miR-34b-5p or miR-470-5p was verified by dual-luciferase reporter assay and RNA immunoprecipitation assay.Results: Our data indicated that Berberine could inhibit CUMS mice depression behaviors and enhance hippocampal neurons growth by targeting miR-34b-5p and miR-470-5p. In addition, we found that BDNF was a target of miR-34b-5p and miR-470-5p. Overexpressed BDNF could reverse the regulation of miR-34b-5p and miR-470-5p on CUMS mice depression behaviors and hippocampal neurons growth. Furthermore, Berberine could promote BDNF expression to regulate CUMS mice depression behaviors and hippocampal neurons growth.Conclusion: Berberine might inhibit the progression of depression disorder by regulating the miR-34b-5p/miR-470-5p/BDNF axis.Keywords: depression disorder, Berberine, miR-34b-5p, miR-470-5p, BDNFZhan YHan JXia JWang XDove Medical Pressarticledepression disorderberberinemir-34b-5pmir-470-5pbdnfNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 17, Pp 613-626 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	depression disorder berberine mir-34b-5p mir-470-5p bdnf Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	depression disorder berberine mir-34b-5p mir-470-5p bdnf Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Zhan Y Han J Xia J Wang X Berberine Suppresses Mice Depression Behaviors and Promotes Hippocampal Neurons Growth Through Regulating the miR-34b-5p/miR-470-5p/BDNF Axis
description	Yuhua Zhan, Jiyang Han, Jing Xia, Xumei Wang Department of Psychiatry, Shengjing Hospital of China Medical University, Shenyang City, Liaoning Province, People’s Republic of ChinaCorrespondence: Xumei WangDepartment of Psychiatry, Shengjing Hospital of China Medical University, Shenyang City, Liaoning Province, People’s Republic of ChinaTel/Fax +86 0249-661566112Email xiaoyema2020@163.comBackground: Berberine has been found to inhibit the progression of depression disorder, but its specific mechanism is still unclear. MicroRNA (miRNA) is considered to play an important role in the progression of depression. However, it is unclear whether Berberine is involved in the regulation of depression progression through miRNA.Methods: The chronic unpredictable mild stress (CUMS) mice model was constructed. Mice depression behaviors were evaluated by sucrose preference test (SPT) and forced swim test (FST). Quantitative real-time PCR was employed to assess the expression of miR-34b-5p, miR-470-5p and brain-derived neurotrophic factor (BDNF). The protein expression of BDNF was examined using Western blot analysis. In addition, the viability and apoptosis of hippocampal neurons were determined using cell counting kit 8 assay, flow cytometry and TUNEL assay. The interaction between BDNF and miR-34b-5p or miR-470-5p was verified by dual-luciferase reporter assay and RNA immunoprecipitation assay.Results: Our data indicated that Berberine could inhibit CUMS mice depression behaviors and enhance hippocampal neurons growth by targeting miR-34b-5p and miR-470-5p. In addition, we found that BDNF was a target of miR-34b-5p and miR-470-5p. Overexpressed BDNF could reverse the regulation of miR-34b-5p and miR-470-5p on CUMS mice depression behaviors and hippocampal neurons growth. Furthermore, Berberine could promote BDNF expression to regulate CUMS mice depression behaviors and hippocampal neurons growth.Conclusion: Berberine might inhibit the progression of depression disorder by regulating the miR-34b-5p/miR-470-5p/BDNF axis.Keywords: depression disorder, Berberine, miR-34b-5p, miR-470-5p, BDNF
format	article
author	Zhan Y Han J Xia J Wang X
author_facet	Zhan Y Han J Xia J Wang X
author_sort	Zhan Y
title	Berberine Suppresses Mice Depression Behaviors and Promotes Hippocampal Neurons Growth Through Regulating the miR-34b-5p/miR-470-5p/BDNF Axis
title_short	Berberine Suppresses Mice Depression Behaviors and Promotes Hippocampal Neurons Growth Through Regulating the miR-34b-5p/miR-470-5p/BDNF Axis
title_full	Berberine Suppresses Mice Depression Behaviors and Promotes Hippocampal Neurons Growth Through Regulating the miR-34b-5p/miR-470-5p/BDNF Axis
title_fullStr	Berberine Suppresses Mice Depression Behaviors and Promotes Hippocampal Neurons Growth Through Regulating the miR-34b-5p/miR-470-5p/BDNF Axis
title_full_unstemmed	Berberine Suppresses Mice Depression Behaviors and Promotes Hippocampal Neurons Growth Through Regulating the miR-34b-5p/miR-470-5p/BDNF Axis
title_sort	berberine suppresses mice depression behaviors and promotes hippocampal neurons growth through regulating the mir-34b-5p/mir-470-5p/bdnf axis
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/7e1eb88fb18b42cfa71f19ea89a18281
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Berberine Suppresses Mice Depression Behaviors and Promotes Hippocampal Neurons Growth Through Regulating the miR-34b-5p/miR-470-5p/BDNF Axis

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