Effects of acute heat stress on protein expression and histone modification in the adrenal gland of male layer-type country chickens

Abstract The adrenal gland responds to heat stress by epinephrine and glucocorticoid release to alleviate the adverse effects. This study investigated the effect of acute heat stress on the protein profile and histone modification in the adrenal gland of layer-type country chickens. A total of 192 r...

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Autores principales:	Hao-Teng Zheng, Zi-Xuan Zhuang, Chao-Jung Chen, Hsin-Yi Liao, Hung-Lin Chen, Huang-Chun Hsueh, Chih-Feng Chen, Shuen-Ei Chen, San-Yuan Huang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7e1f28897ddf4ecab2c76ec7e1d24699
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spelling	oai:doaj.org-article:7e1f28897ddf4ecab2c76ec7e1d246992021-12-02T17:04:35ZEffects of acute heat stress on protein expression and histone modification in the adrenal gland of male layer-type country chickens10.1038/s41598-021-85868-12045-2322https://doaj.org/article/7e1f28897ddf4ecab2c76ec7e1d246992021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85868-1https://doaj.org/toc/2045-2322Abstract The adrenal gland responds to heat stress by epinephrine and glucocorticoid release to alleviate the adverse effects. This study investigated the effect of acute heat stress on the protein profile and histone modification in the adrenal gland of layer-type country chickens. A total of 192 roosters were subject to acute heat stress and thereafter classified into a resistant or susceptible group according to body temperature change. The iTRAQ analysis identified 80 differentially expressed proteins, in which the resistant group had a higher level of somatostatin and hydroxy-δ-5-steroid dehydrogenase but a lower parathymosin expression in accordance with the change of serum glucocorticoid levels. Histone modification analysis identified 115 histone markers. The susceptible group had a higher level of tri-methylation of histone H3 lysine 27 (H3K27me3) and showed a positive crosstalk with K36me and K37me in the H3 tails. The differential changes of body temperature projected in physiological regulation at the hypothalamus–pituitary–adrenal axis suggest the genetic heterogeneity in basic metabolic rate and efficiency for heat dissipation to acclimate to thermal stress and maintain body temperature homeostasis. The alteration of adrenal H3K27me3 level was associated with the endocrine function of adrenal gland and may contribute to the thermotolerance of chickens.Hao-Teng ZhengZi-Xuan ZhuangChao-Jung ChenHsin-Yi LiaoHung-Lin ChenHuang-Chun HsuehChih-Feng ChenShuen-Ei ChenSan-Yuan HuangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hao-Teng Zheng Zi-Xuan Zhuang Chao-Jung Chen Hsin-Yi Liao Hung-Lin Chen Huang-Chun Hsueh Chih-Feng Chen Shuen-Ei Chen San-Yuan Huang Effects of acute heat stress on protein expression and histone modification in the adrenal gland of male layer-type country chickens
description	Abstract The adrenal gland responds to heat stress by epinephrine and glucocorticoid release to alleviate the adverse effects. This study investigated the effect of acute heat stress on the protein profile and histone modification in the adrenal gland of layer-type country chickens. A total of 192 roosters were subject to acute heat stress and thereafter classified into a resistant or susceptible group according to body temperature change. The iTRAQ analysis identified 80 differentially expressed proteins, in which the resistant group had a higher level of somatostatin and hydroxy-δ-5-steroid dehydrogenase but a lower parathymosin expression in accordance with the change of serum glucocorticoid levels. Histone modification analysis identified 115 histone markers. The susceptible group had a higher level of tri-methylation of histone H3 lysine 27 (H3K27me3) and showed a positive crosstalk with K36me and K37me in the H3 tails. The differential changes of body temperature projected in physiological regulation at the hypothalamus–pituitary–adrenal axis suggest the genetic heterogeneity in basic metabolic rate and efficiency for heat dissipation to acclimate to thermal stress and maintain body temperature homeostasis. The alteration of adrenal H3K27me3 level was associated with the endocrine function of adrenal gland and may contribute to the thermotolerance of chickens.
format	article
author	Hao-Teng Zheng Zi-Xuan Zhuang Chao-Jung Chen Hsin-Yi Liao Hung-Lin Chen Huang-Chun Hsueh Chih-Feng Chen Shuen-Ei Chen San-Yuan Huang
author_facet	Hao-Teng Zheng Zi-Xuan Zhuang Chao-Jung Chen Hsin-Yi Liao Hung-Lin Chen Huang-Chun Hsueh Chih-Feng Chen Shuen-Ei Chen San-Yuan Huang
author_sort	Hao-Teng Zheng
title	Effects of acute heat stress on protein expression and histone modification in the adrenal gland of male layer-type country chickens
title_short	Effects of acute heat stress on protein expression and histone modification in the adrenal gland of male layer-type country chickens
title_full	Effects of acute heat stress on protein expression and histone modification in the adrenal gland of male layer-type country chickens
title_fullStr	Effects of acute heat stress on protein expression and histone modification in the adrenal gland of male layer-type country chickens
title_full_unstemmed	Effects of acute heat stress on protein expression and histone modification in the adrenal gland of male layer-type country chickens
title_sort	effects of acute heat stress on protein expression and histone modification in the adrenal gland of male layer-type country chickens
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7e1f28897ddf4ecab2c76ec7e1d24699
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Effects of acute heat stress on protein expression and histone modification in the adrenal gland of male layer-type country chickens

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