Quantitative trait loci and transcriptome signatures associated with avian heritable resistance to Campylobacter

Abstract Campylobacter is the leading cause of bacterial foodborne gastroenteritis worldwide. Handling or consumption of contaminated poultry meat is a key risk factor for human campylobacteriosis. One potential control strategy is to select poultry with increased resistance to Campylobacter. We ass...

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Autores principales:	Androniki Psifidi, Andreas Kranis, Lisa M. Rothwell, Abi Bremner, Kay Russell, Diego Robledo, Stephen J. Bush, Mark Fife, Paul M. Hocking, Georgios Banos, David A. Hume, Jim Kaufman, Richard A. Bailey, Santiago Avendano, Kellie A. Watson, Pete Kaiser, Mark. P. Stevens
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7e1f6472c34c49b9b1c83a773c652d42
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spelling	oai:doaj.org-article:7e1f6472c34c49b9b1c83a773c652d422021-12-02T14:01:37ZQuantitative trait loci and transcriptome signatures associated with avian heritable resistance to Campylobacter10.1038/s41598-020-79005-72045-2322https://doaj.org/article/7e1f6472c34c49b9b1c83a773c652d422021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79005-7https://doaj.org/toc/2045-2322Abstract Campylobacter is the leading cause of bacterial foodborne gastroenteritis worldwide. Handling or consumption of contaminated poultry meat is a key risk factor for human campylobacteriosis. One potential control strategy is to select poultry with increased resistance to Campylobacter. We associated high-density genome-wide genotypes (600K single nucleotide polymorphisms) of 3000 commercial broilers with Campylobacter load in their caeca. Trait heritability was modest but significant (h2 = 0.11 ± 0.03). Results confirmed quantitative trait loci (QTL) on chromosomes 14 and 16 previously identified in inbred chicken lines, and detected two additional QTLs on chromosomes 19 and 26. RNA-Seq analysis of broilers at the extremes of colonisation phenotype identified differentially transcribed genes within the QTL on chromosome 16 and proximal to the major histocompatibility complex (MHC) locus. We identified strong cis-QTLs located within MHC suggesting the presence of cis-acting variation in MHC class I and II and BG genes. Pathway and network analyses implicated cooperative functional pathways and networks in colonisation, including those related to antigen presentation, innate and adaptive immune responses, calcium, and renin–angiotensin signalling. While co-selection for enhanced resistance and other breeding goals is feasible, the frequency of resistance-associated alleles was high in the population studied and non-genetic factors significantly influenced Campylobacter colonisation.Androniki PsifidiAndreas KranisLisa M. RothwellAbi BremnerKay RussellDiego RobledoStephen J. BushMark FifePaul M. HockingGeorgios BanosDavid A. HumeJim KaufmanRichard A. BaileySantiago AvendanoKellie A. WatsonPete KaiserMark. P. StevensNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Androniki Psifidi Andreas Kranis Lisa M. Rothwell Abi Bremner Kay Russell Diego Robledo Stephen J. Bush Mark Fife Paul M. Hocking Georgios Banos David A. Hume Jim Kaufman Richard A. Bailey Santiago Avendano Kellie A. Watson Pete Kaiser Mark. P. Stevens Quantitative trait loci and transcriptome signatures associated with avian heritable resistance to Campylobacter
description	Abstract Campylobacter is the leading cause of bacterial foodborne gastroenteritis worldwide. Handling or consumption of contaminated poultry meat is a key risk factor for human campylobacteriosis. One potential control strategy is to select poultry with increased resistance to Campylobacter. We associated high-density genome-wide genotypes (600K single nucleotide polymorphisms) of 3000 commercial broilers with Campylobacter load in their caeca. Trait heritability was modest but significant (h2 = 0.11 ± 0.03). Results confirmed quantitative trait loci (QTL) on chromosomes 14 and 16 previously identified in inbred chicken lines, and detected two additional QTLs on chromosomes 19 and 26. RNA-Seq analysis of broilers at the extremes of colonisation phenotype identified differentially transcribed genes within the QTL on chromosome 16 and proximal to the major histocompatibility complex (MHC) locus. We identified strong cis-QTLs located within MHC suggesting the presence of cis-acting variation in MHC class I and II and BG genes. Pathway and network analyses implicated cooperative functional pathways and networks in colonisation, including those related to antigen presentation, innate and adaptive immune responses, calcium, and renin–angiotensin signalling. While co-selection for enhanced resistance and other breeding goals is feasible, the frequency of resistance-associated alleles was high in the population studied and non-genetic factors significantly influenced Campylobacter colonisation.
format	article
author	Androniki Psifidi Andreas Kranis Lisa M. Rothwell Abi Bremner Kay Russell Diego Robledo Stephen J. Bush Mark Fife Paul M. Hocking Georgios Banos David A. Hume Jim Kaufman Richard A. Bailey Santiago Avendano Kellie A. Watson Pete Kaiser Mark. P. Stevens
author_facet	Androniki Psifidi Andreas Kranis Lisa M. Rothwell Abi Bremner Kay Russell Diego Robledo Stephen J. Bush Mark Fife Paul M. Hocking Georgios Banos David A. Hume Jim Kaufman Richard A. Bailey Santiago Avendano Kellie A. Watson Pete Kaiser Mark. P. Stevens
author_sort	Androniki Psifidi
title	Quantitative trait loci and transcriptome signatures associated with avian heritable resistance to Campylobacter
title_short	Quantitative trait loci and transcriptome signatures associated with avian heritable resistance to Campylobacter
title_full	Quantitative trait loci and transcriptome signatures associated with avian heritable resistance to Campylobacter
title_fullStr	Quantitative trait loci and transcriptome signatures associated with avian heritable resistance to Campylobacter
title_full_unstemmed	Quantitative trait loci and transcriptome signatures associated with avian heritable resistance to Campylobacter
title_sort	quantitative trait loci and transcriptome signatures associated with avian heritable resistance to campylobacter
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7e1f6472c34c49b9b1c83a773c652d42
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Quantitative trait loci and transcriptome signatures associated with avian heritable resistance to Campylobacter

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