Rbm24 displays dynamic functions required for myogenic differentiation during muscle regeneration

Abstract Skeletal muscle has a remarkable capacity of regeneration after injury, but the regulatory network underlying this repair process remains elusive. RNA-binding proteins play key roles in the post-transcriptional regulation of gene expression and the maintenance of tissue homeostasis and plas...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Raphaëlle Grifone, Audrey Saquet, Manon Desgres, Claudia Sangiorgi, Caterina Gargano, Zhenlin Li, Dario Coletti, De-Li Shi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7e2df0f3ea7e404ead019033bc8f3956
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7e2df0f3ea7e404ead019033bc8f3956
record_format	dspace
spelling	oai:doaj.org-article:7e2df0f3ea7e404ead019033bc8f39562021-12-02T16:49:07ZRbm24 displays dynamic functions required for myogenic differentiation during muscle regeneration10.1038/s41598-021-88563-32045-2322https://doaj.org/article/7e2df0f3ea7e404ead019033bc8f39562021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88563-3https://doaj.org/toc/2045-2322Abstract Skeletal muscle has a remarkable capacity of regeneration after injury, but the regulatory network underlying this repair process remains elusive. RNA-binding proteins play key roles in the post-transcriptional regulation of gene expression and the maintenance of tissue homeostasis and plasticity. Rbm24 regulates myogenic differentiation during early development, but its implication in adult muscle is poorly understood. Here we show that it exerts multiple functions in muscle regeneration. Consistent with its dynamic subcellular localization during embryonic muscle development, Rbm24 also displays cytoplasm to nucleus translocation during C2C12 myoblast differentiation. In adult mice, Rbm24 mRNA is enriched in slow-twitch muscles along with myogenin mRNA. The protein displays nuclear localization in both slow and fast myofibers. Upon injury, Rbm24 is rapidly upregulated in regenerating myofibers and accumulates in the myonucleus of nascent myofibers. Through satellite cell transplantation, we demonstrate that Rbm24 functions sequentially to regulate myogenic differentiation and muscle regeneration. It is required for myogenin expression at early stages of muscle injury and for muscle-specific pre-mRNA alternative splicing at late stages of regeneration. These results identify Rbm24 as a multifaceted regulator of myoblast differentiation. They provide insights into the molecular pathway orchestrating the expression of myogenic factors and muscle functional proteins during regeneration.Raphaëlle GrifoneAudrey SaquetManon DesgresClaudia SangiorgiCaterina GarganoZhenlin LiDario ColettiDe-Li ShiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Raphaëlle Grifone Audrey Saquet Manon Desgres Claudia Sangiorgi Caterina Gargano Zhenlin Li Dario Coletti De-Li Shi Rbm24 displays dynamic functions required for myogenic differentiation during muscle regeneration
description	Abstract Skeletal muscle has a remarkable capacity of regeneration after injury, but the regulatory network underlying this repair process remains elusive. RNA-binding proteins play key roles in the post-transcriptional regulation of gene expression and the maintenance of tissue homeostasis and plasticity. Rbm24 regulates myogenic differentiation during early development, but its implication in adult muscle is poorly understood. Here we show that it exerts multiple functions in muscle regeneration. Consistent with its dynamic subcellular localization during embryonic muscle development, Rbm24 also displays cytoplasm to nucleus translocation during C2C12 myoblast differentiation. In adult mice, Rbm24 mRNA is enriched in slow-twitch muscles along with myogenin mRNA. The protein displays nuclear localization in both slow and fast myofibers. Upon injury, Rbm24 is rapidly upregulated in regenerating myofibers and accumulates in the myonucleus of nascent myofibers. Through satellite cell transplantation, we demonstrate that Rbm24 functions sequentially to regulate myogenic differentiation and muscle regeneration. It is required for myogenin expression at early stages of muscle injury and for muscle-specific pre-mRNA alternative splicing at late stages of regeneration. These results identify Rbm24 as a multifaceted regulator of myoblast differentiation. They provide insights into the molecular pathway orchestrating the expression of myogenic factors and muscle functional proteins during regeneration.
format	article
author	Raphaëlle Grifone Audrey Saquet Manon Desgres Claudia Sangiorgi Caterina Gargano Zhenlin Li Dario Coletti De-Li Shi
author_facet	Raphaëlle Grifone Audrey Saquet Manon Desgres Claudia Sangiorgi Caterina Gargano Zhenlin Li Dario Coletti De-Li Shi
author_sort	Raphaëlle Grifone
title	Rbm24 displays dynamic functions required for myogenic differentiation during muscle regeneration
title_short	Rbm24 displays dynamic functions required for myogenic differentiation during muscle regeneration
title_full	Rbm24 displays dynamic functions required for myogenic differentiation during muscle regeneration
title_fullStr	Rbm24 displays dynamic functions required for myogenic differentiation during muscle regeneration
title_full_unstemmed	Rbm24 displays dynamic functions required for myogenic differentiation during muscle regeneration
title_sort	rbm24 displays dynamic functions required for myogenic differentiation during muscle regeneration
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7e2df0f3ea7e404ead019033bc8f3956
work_keys_str_mv	AT raphaellegrifone rbm24displaysdynamicfunctionsrequiredformyogenicdifferentiationduringmuscleregeneration AT audreysaquet rbm24displaysdynamicfunctionsrequiredformyogenicdifferentiationduringmuscleregeneration AT manondesgres rbm24displaysdynamicfunctionsrequiredformyogenicdifferentiationduringmuscleregeneration AT claudiasangiorgi rbm24displaysdynamicfunctionsrequiredformyogenicdifferentiationduringmuscleregeneration AT caterinagargano rbm24displaysdynamicfunctionsrequiredformyogenicdifferentiationduringmuscleregeneration AT zhenlinli rbm24displaysdynamicfunctionsrequiredformyogenicdifferentiationduringmuscleregeneration AT dariocoletti rbm24displaysdynamicfunctionsrequiredformyogenicdifferentiationduringmuscleregeneration AT delishi rbm24displaysdynamicfunctionsrequiredformyogenicdifferentiationduringmuscleregeneration
_version_	1718383405412909056

Rbm24 displays dynamic functions required for myogenic differentiation during muscle regeneration

Ejemplares similares