Bisphenol A-mediated suppression of LPL gene expression inhibits triglyceride accumulation during adipogenic differentiation of human adult stem cells.

The endocrine disrupting chemical, bisphenol A (BPA), has been shown to accelerate the rate of adipogenesis and increase the amount of triglyceride accumulation during differentiation of 3T3-L1 preadipocytes. The objective of this study was to investigate if that observation is mirrored in human pri...

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Autores principales:	Chris Linehan, Sanjeev Gupta, Afshin Samali, Lynn O'Connor
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7e3197cdba07459d815d50dd2d06e8f1
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spelling	oai:doaj.org-article:7e3197cdba07459d815d50dd2d06e8f12021-11-18T07:17:27ZBisphenol A-mediated suppression of LPL gene expression inhibits triglyceride accumulation during adipogenic differentiation of human adult stem cells.1932-620310.1371/journal.pone.0036109https://doaj.org/article/7e3197cdba07459d815d50dd2d06e8f12012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22662114/?tool=EBIhttps://doaj.org/toc/1932-6203The endocrine disrupting chemical, bisphenol A (BPA), has been shown to accelerate the rate of adipogenesis and increase the amount of triglyceride accumulation during differentiation of 3T3-L1 preadipocytes. The objective of this study was to investigate if that observation is mirrored in human primary cells. Here we investigated the effect of BPA on adipogenesis in cultured human primary adult stem cells. Continuous exposure to BPA throughout the 14 days of differentiation dramatically reduced triglyceride accumulation and suppressed gene transcription of the lipogenic enzyme, lipoprotein lipase (LPL). Results presented in the present study show for the first time that BPA can reduce triglyceride accumulation during adipogenesis by attenuating the expression of LPL gene transcription. Also, by employing image cytometric analysis rather than conventional Oil red O staining techniques we show that BPA regulates triglyceride accumulation in a manner which does not appear to effect adipogenesis per se.Chris LinehanSanjeev GuptaAfshin SamaliLynn O'ConnorPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e36109 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chris Linehan Sanjeev Gupta Afshin Samali Lynn O'Connor Bisphenol A-mediated suppression of LPL gene expression inhibits triglyceride accumulation during adipogenic differentiation of human adult stem cells.
description	The endocrine disrupting chemical, bisphenol A (BPA), has been shown to accelerate the rate of adipogenesis and increase the amount of triglyceride accumulation during differentiation of 3T3-L1 preadipocytes. The objective of this study was to investigate if that observation is mirrored in human primary cells. Here we investigated the effect of BPA on adipogenesis in cultured human primary adult stem cells. Continuous exposure to BPA throughout the 14 days of differentiation dramatically reduced triglyceride accumulation and suppressed gene transcription of the lipogenic enzyme, lipoprotein lipase (LPL). Results presented in the present study show for the first time that BPA can reduce triglyceride accumulation during adipogenesis by attenuating the expression of LPL gene transcription. Also, by employing image cytometric analysis rather than conventional Oil red O staining techniques we show that BPA regulates triglyceride accumulation in a manner which does not appear to effect adipogenesis per se.
format	article
author	Chris Linehan Sanjeev Gupta Afshin Samali Lynn O'Connor
author_facet	Chris Linehan Sanjeev Gupta Afshin Samali Lynn O'Connor
author_sort	Chris Linehan
title	Bisphenol A-mediated suppression of LPL gene expression inhibits triglyceride accumulation during adipogenic differentiation of human adult stem cells.
title_short	Bisphenol A-mediated suppression of LPL gene expression inhibits triglyceride accumulation during adipogenic differentiation of human adult stem cells.
title_full	Bisphenol A-mediated suppression of LPL gene expression inhibits triglyceride accumulation during adipogenic differentiation of human adult stem cells.
title_fullStr	Bisphenol A-mediated suppression of LPL gene expression inhibits triglyceride accumulation during adipogenic differentiation of human adult stem cells.
title_full_unstemmed	Bisphenol A-mediated suppression of LPL gene expression inhibits triglyceride accumulation during adipogenic differentiation of human adult stem cells.
title_sort	bisphenol a-mediated suppression of lpl gene expression inhibits triglyceride accumulation during adipogenic differentiation of human adult stem cells.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/7e3197cdba07459d815d50dd2d06e8f1
work_keys_str_mv	AT chrislinehan bisphenolamediatedsuppressionoflplgeneexpressioninhibitstriglycerideaccumulationduringadipogenicdifferentiationofhumanadultstemcells AT sanjeevgupta bisphenolamediatedsuppressionoflplgeneexpressioninhibitstriglycerideaccumulationduringadipogenicdifferentiationofhumanadultstemcells AT afshinsamali bisphenolamediatedsuppressionoflplgeneexpressioninhibitstriglycerideaccumulationduringadipogenicdifferentiationofhumanadultstemcells AT lynnoconnor bisphenolamediatedsuppressionoflplgeneexpressioninhibitstriglycerideaccumulationduringadipogenicdifferentiationofhumanadultstemcells
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Bisphenol A-mediated suppression of LPL gene expression inhibits triglyceride accumulation during adipogenic differentiation of human adult stem cells.

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